簡易檢索 / 詳目顯示

研究生: 朱以恩
Chu, Yi-En
論文名稱: 渠道中藻毯對兩種土霉味物質的貢獻及模擬
The Contribution and Simulation of Two Earthy and Musty Compounds of Cyanobacterial Mat in Channels
指導教授: 林財富
Lin, Tsair-Fuh
學位類別: 碩士
Master
系所名稱: 工學院 - 環境工程學系
Department of Environmental Engineering
論文出版年: 2026
畢業學年度: 114
語文別: 中文
論文頁數: 204
中文關鍵詞: 藍綠菌2-MIBGSM質量平衡模型一維平流延散方程式藻毯臭味產生通量
外文關鍵詞: Cyanobacterial mat, 2-Methylisoborneol (2-MIB), Geosmin (GSM), One-dimensional advection-dispersion-reaction model, Odor emission flux
相關次數: 點閱:4下載:0
分享至:
查詢本校圖書館目錄 查詢臺灣博碩士論文知識加值系統 勘誤回報
  • 近年來,氣候變遷及極端氣候現象日益明顯,使水體優養化程度升高,導致水中因藍綠菌所引起的藻華頻率與強度增加,水體中臭味事件也日益頻繁出現。2-methylisoborneol (2-MIB) 和Geosmin (GSM) 是台灣及世界水源中最常見的藍綠菌臭味物質,具土霉味、低閾值特性,常影響民眾飲水感覺、進而對於民生用水安全產生疑慮;然而其在水庫及渠道等水體中生成及傳輸機制仍需進一步探討。
    本研究主要探討藻毯在水中對2-MIB及GSM濃度的貢獻,量化藻毯的2-MIB及GSM生成通量,並以質量平衡模型模擬分析,探討2-MIB及GSM在渠道中的濃度變化。研究使用具產2-MIB及GSM的大崗山淨水場及葫蘆埤渠道藍綠菌藻毯進行通量實驗,顯微鏡分析顯示,藻毯中富含Pseudanabaena、Oscillatoria、Potamolineam與Lyngbya等藻,DNA及化學分析顯示藻毯具產生2-MIB及GSM能力。藻毯通量實驗顯示2-MIB和GSM兩臭味物質濃度會隨培養時間產生變化,2-MIB的臭味產生通量,最大值約為600 ng/m2/h,平均值約為200 ng/m2/h;GSM臭味產生通量,最大值約為28,000 ng/m2/h,而平均值約為200 ng/m2/h。
    本研究以一維平流延散方程式,結合Crank-Nicolson數值法,並應用實驗室求得之藻毯臭味產生通量及2-MIB一階生物降解係數、現場量測之水理參數及渠道性質、文獻參考之臭味一階生物降解係數、理論計算之延散係數及揮發參數,以及李(2026)藻毯辨識技術獲取懸浮藻毯水面之面積比例,模擬三爺宮溪水體5公里段的2-MIB和GSM空間變化,並與實際場址分析數據比對。研究結果顯示,其中2-MIB於兩次採樣之NRMSE約為8-15%;GSM則因採樣日期及通量情境不同而有所差異,NRMSE約為9.77-47.3%。以簡易模式而言,其擬合情形大致上皆相當好。
    研究中並建立懸浮與底棲藻毯產臭能力累積機率圖數據資料庫,可提供藻毯辨識系統之資料庫應用。透過本研究,除可量化藻毯在2-MIB與GSM污染事件中的角色,亦可作為影像辨識與水源預警系統的基礎,對水源管理與提升應變效率具有實質應用價值。

    Cyanobacterial blooms have become increasingly frequent due to eutrophication and climate change, resulting in recurrent taste-and-odor events in drinking water sources. Among the odor-causing compounds, 2-methylisoborneol (2-MIB) and geosmin (GSM) are the most frequently detected compounds worldwide. However, the contribution of cyanobacterial mats to odor production and the transport behavior of these compounds in open channels remain poorly understood. This study quantified the odor emission fluxes from cyanobacterial mats through laboratory cultivation experiments and incorporated the experimental results into a one-dimensional advection–dispersion–reaction model to simulate odor transport in Sanyegong Creek, Taiwan. Microscopic observation, gas chromatography-mass spectrometry (GC/MS) analysis, and quantitative polymerase chain reaction (qPCR) were employed to characterize cyanobacterial species, odor compounds, and odor synthesis genes. Representative odor emission fluxes were determined to be approximately 200 ng/m2/h for both 2-MIB and GSM, whereas maximum fluxes reached approximately 600 ng/m2/h for 2-MIB and 28,000 ng/m2/h for GSM. Model validation demonstrated satisfactory agreement between simulated and observed concentrations, with normalized root mean square errors (NRMSE) generally between 8% and 15% for 2-MIB. In addition, probability distribution databases of odor content in suspended and benthic cyanobacterial mats were established and integrated with an AI-based image recognition system to estimate potential odor release under different risk scenarios. The proposed framework provides a practical tool for predicting odor events and supporting water source management.

    中文摘要 i Extended Abstract iii 誌謝 ix 目錄 xi 表目錄 xvii 圖目錄 xx 第一章 前言 1 1.1 研究源起與動機 1 1.2 研究目的 3 第二章 文獻回顧 4 2.1 藍綠菌 4 2.2 臭味物質2-MIB及GSM 6 2.2.1 臭味物質(2-MIB, GSM)來源 9 2.2.2 2-MIB及GSM於淨水程序之去除 15 2.3 藻毯 (Algal mat, Cyanobacterial mat) 17 2.3.1 藻毯的生成及穩定影響因子 19 2.3.1.1 光照強度 19 2.3.1.2 水溫與季節 19 2.3.1.3 營養鹽濃度 20 2.3.1.4 水力條件 20 2.3.2 藻毯於自然水體中的影響 21 2.3.2.1 正面影響 21 2.3.2.2 負面影響 22 2.4 模擬模型分類 23 2.5 一維平流延散模型 28 2.6 敏感度分析 (Sensitivity Analysis) 34 2.7 分子生物技術 35 2.7.1 即時定量聚合酶連鎖反應 (Real-time Polymerase Chain Reaction, qPCR) 35 2.7.2 mibC基因和2-MIB臭味關聯性 38 2.7.3 geoA基因和GSM臭味 39 第三章 研究方法與步驟 41 3.1 研究架構 41 3.2 實驗設計及設備 42 3.2.1 實驗方法 42 3.2.1.1 藻毯培養實驗配置及條件 42 3.2.2 實驗設備 46 3.3 研究場址 46 3.3.1 高雄大崗山給水場 47 3.3.2 台南市官田區葫蘆埤 47 3.3.3 台南市三爺宮溪 48 3.4 臭味物質分析方法 49 3.4.1 一般水體臭味物質濃度分析 49 3.4.2 藻毯中臭味物質總量分析 49 3.4.2.1 藻毯中臭味物質萃取 50 3.4.2.2 藻毯中臭味物質濃度分析 50 3.4.3 實驗試劑 50 3.4.4 實驗設備 51 3.4.5 實驗步驟 52 3.5 DNA萃取 53 3.5.1 一般水樣萃取Plant Genomic DNA Extraction Mini Kit 53 3.5.1.1 設備與材料 53 3.5.1.2 實驗步驟 54 3.5.2 藻毯樣本萃取SPINeasyTM DNA Pro Kit for Soil 56 3.5.2.1 設備與材料 56 3.5.2.2 實驗步驟 56 3.6 即時聚合酶連鎖反應 (Real-time polymerase chain reaction, qPCR) 58 3.6.1 設備與藥劑 58 3.6.2 實驗步驟 60 3.7 藻毯葉綠素a (Chlorophyll a) 測定 62 3.7.1 實驗步驟 62 3.7.2 設備與藥劑 62 3.8 模型模擬 63 3.8.1 Crank-Nicolson數值方法解 63 3.8.2 簡易平流模型 70 3.8.3 敏感度分析方法 71 第四章 結果與討論 73 4.1 藻毯臭味物質產生通量 73 4.1.1 臭味物質濃度變化趨勢 73 4.1.1.1 2-MIB濃度變化 73 4.1.1.2 GSM濃度變化 78 4.1.2 葉綠素a測量與藻毯觀察 83 4.1.3 臭味物質通量計算 85 4.1.3.1 2-MIB產生通量 86 4.1.3.2 GSM產生通量 91 4.2 藍綠菌藻毯與臭味質量及合成基因之關聯 96 4.2.1 藻毯2-MIB質量與mibC基因機率分布圖 98 4.2.2 GSM質量與geoA/CGeo基因機率分布圖 102 4.2.3 臭味質量機率分布圖之應用 105 4.3 一維延散模型模擬 108 4.3.1 台南市三爺宮溪 109 4.3.1.1 模式參數設定 115 4.3.1.2 藻毯覆蓋率設定 117 4.3.2 三爺宮溪模擬結果 120 4.3.3 情境分析 132 4.3.3.1 藻毯覆蓋率及臭味通量對渠道臭味之影響 132 4.3.4 敏感度分析 135 第五章 結論與建議 139 5.1 結論 139 5.2 建議 141 第六章 附錄 142 6.1 渠道及藻毯調查 142 6.1.1 渠道及藻毯場址調查 142 6.1.2 水樣數據總表 143 6.1.3 藻毯數據總表 145 6.2 模擬模型 Python Code 157 參考文獻 170

    Agency, U. S. E. P. (2009). Guidance on the Development, Evaluation, and Application of Environmental Models (EPA/100/K-09/003). U. S. E. P. Agency. https://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=P1003E4R.TXT
    AlgaeBase. https://www.algaebase.org/
    Bai, J., Xiao, R., Cui, B., Zhang, K., Wang, Q., Liu, X., Gao, H., & Huang, L. (2011). Assessment of heavy metal pollution in wetland soils from the young and old reclaimed regions in the Pearl River Estuary, South China. Environmental pollution, 159(3), 817–824.
    Berman-Frank, I., Lundgren, P., & Falkowski, P. (2003). Nitrogen fixation and photosynthetic oxygen evolution in cyanobacteria. Research in microbiology, 154(3), 157–164.
    Bolhuis, H., Cretoiu, M. S., & Stal, L. J. (2014). Molecular ecology of microbial mats. FEMS Microbiology Ecology, 90(2), 335–350.
    Bruder, S., Babbar-Sebens, M., Tedesco, L., & Soyeux, E. (2014). Use of fuzzy logic models for prediction of taste and odor compounds in algal bloom-affected inland water bodies. Environmental monitoring and assessment, 186(3), 1525–1545.
    Burden, A., Burden, R., & Faires, J. (2016). Numerical Analysis, 10th ed. https://doi.org/10.13140/2.1.4830.2406
    Bustin, S. A., Benes, V., Garson, J. A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M. W., & Shipley, G. L. (2009). The MIQE Guidelines: M inimum I nformation for Publication of Q uantitative Real-Time PCR E xperiments. In: Oxford University Press.
    Cao, P., Xu, F., Gao, S., Baoligao, B., Li, X., Mu, X., Mendes, A., & Shang, X. (2022). Experimental study on the impact of pulsed flow velocity on the scouring of benthic algae from a mountainous river. Water, 14(19), 3150.
    Carpenter-Boggs, L., Loynachan, T., & Stahl, P. (1995). Spore germination of Gigaspora margarita stimulated by volatiles of soil-isolated actinomycetes. Soil biology and biochemistry, 27(11), 1445–1451.
    Carter, M. C., Weber Jr, W. J., & Olmstead, K. P. (1992). Effects of background dissolved organic matter on TCE adsorption by GAC. Journal‐American Water Works Association, 84(8), 81–91.
    Catherine, Q., Susanna, W., Isidora, E.-S., Mark, H., Aurelie, V., & Jean-François, H. (2013). A review of current knowledge on toxic benthic freshwater cyanobacteria–ecology, toxin production and risk management. Water Research, 47(15), 5464–5479.
    Chen, G., Dussert, B., & Suffet, I. (1997). Evaluation of granular activated carbons for removal of methylisoborneol to below odor threshold concentration in drinking water. Water Research, 31(5), 1155–1163.
    Chen, X., Li, Z., Xu, H., Qiu, L., Fan, L., Meng, S., Gao, Z., & Song, C. (2024). Regulation of salinity to inhibit 2-methylisoborneol and geosmin: Insights from spatial-scale research in coastal areas of China. Frontiers in Environmental Science, 12, 1433586.
    Chen, Y.-M., Hobson, P., Burch, M. D., & Lin, T.-F. (2010). In situ measurement of odor compound production by benthic cyanobacteria. Journal of Environmental Monitoring, 12(3), 769–775.
    Chiu, Y.-T., Yen, H.-K., & Lin, T.-F. (2016). An alternative method to quantify 2-MIB producing cyanobacteria in drinking water reservoirs: Method development and field applications. Environmental Research, 151, 618–627.
    Chorus, I., & Welker, M. (2021). Toxic cyanobacteria in water: a guide to their public health consequences, monitoring and management. Taylor & Francis.
    Cook, D., Newcombe, G., & Sztajnbok, P. (1998). Optimising PAC dosing to remove MIB and geosmin in four Adelaide metropolitan water treatment plants. 61st Annual Water Industry Engineers and Operators’ Association Conference,
    Crank, J., & Nicolson, P. (1947). A practical method for numerical evaluation of solutions of partial differential equations of the heat-conduction type. Mathematical proceedings of the Cambridge philosophical society,
    Devi, A., Chiu, Y.-T., Hsueh, H.-T., & Lin, T.-F. (2021). Quantitative PCR based detection system for cyanobacterial geosmin/2-methylisoborneol (2-MIB) events in drinking water sources: Current status and challenges. Water Research, 188, 116478.
    Dvořák, P., Hašler, P., Casamatta, D. A., & Poulíčková, A. (2021). Underestimated cyanobacterial diversity: Trends and perspectives of research in tropical environments. Fottea, 21(2), 110–127.
    Dzialowski, A. R., Smith, V. H., Huggins, D. G., deNoyelles, F., Lim, N.-C., Baker, D. S., & Beury, J. H. (2009). Development of predictive models for geosmin-related taste and odor in Kansas, USA, drinking water reservoirs. Water Research, 43(11), 2829–2840. https://doi.org/https://doi.org/10.1016/j.watres.2009.04.001
    Etesami, H. (2025). Resilient pioneers: The ecological role of cyanobacteria in desert ecosystems. Applied Soil Ecology, 212, 106173.
    Flynn, K. F., Chapra, S. C., & Suplee, M. W. (2013). Modeling the lateral variation of bottom-attached algae in rivers. Ecological Modelling, 267, 11–25. https://doi.org/https://doi.org/10.1016/j.ecolmodel.2013.07.011
    Frank, A., & Groll, M. (2017). The methylerythritol phosphate pathway to isoprenoids. Chemical Reviews, 117(8), 5675–5703.
    Franklin, H. M., Podduturi, R., Jørgensen, N. O., Roberts, D. T., Schlüter, L., & Burford, M. A. (2023). Potential sources and producers of 2-methylisoborneol and geosmin in a river supplying a drinking water treatment plant. Chemical Engineering Journal Advances, 14, 100455.
    Fuller, E. N., Schettler, P. D., & Giddings, J. C. (1966). New method for prediction of binary gas-phase diffusion coefficients. Industrial & Engineering Chemistry, 58(5), 18–27.
    Gaget, V., Almuhtaram, H., Kibuye, F., Hobson, P., Zamyadi, A., Wert, E., & Brookes, J. D. (2022). Benthic cyanobacteria: A utility-centred field study. Harmful Algae, 113, 102185. https://doi.org/https://doi.org/10.1016/j.hal.2022.102185
    Garcia-Pichel, F., Belnap, J., Neuer, S., & Schanz, F. (2003). Estimates of global cyanobacterial biomass and its distribution. Algological Studies, 109(1), 213–227.
    Garstecki, B., & Wells, S. (2023). Modeling cyanotoxin production, fate, and transport in surface water bodies using CE-QUAL-W2. Environments, 10(7), 122.
    Gerber, N., & Lechevalier, H. (1965). Geosmin, an earthy-smelling substance isolated from actinomycetes. Applied microbiology, 13(6), 935–938.
    Gerber, N. N. (1969). A volatile metabolite of actinomycetes, 2-methylisoborneol. J Antibiot (Tokyo), 22(10), 508–509. https://doi.org/10.7164/antibiotics.22.508
    Hamby, D. M. (1994). A review of techniques for parameter sensitivity analysis of environmental models. Environmental monitoring and assessment, 32(2), 135–154.
    Hancke, K., & Glud, R. N. (2004). Temperature effects on respiration and photosynthesis in three diatom-dominated benthic communities. Aquatic Microbial Ecology, 37, 265–281.
    Hayduk, W., & Laudie, H. (1974). Prediction of diffusion coefficients for nonelectrolytes in dilute aqueous solutions. AIChE Journal, 20(3), 611–615. https://doi.org/https://doi.org/10.1002/aic.690200329
    Ho, L., Hoefel, D., Bock, F., Saint, C. P., & Newcombe, G. (2007). Biodegradation rates of 2-methylisoborneol (MIB) and geosmin through sand filters and in bioreactors. Chemosphere, 66(11), 2210–2218.
    Hsieh, W.-H., Chang, D.-W., & Lin, T.-F. (2014). Occurrence and removal of earthy-musty odorants in two waterworks in Kinmen Island, Taiwan. Journal of Hazardous, Toxic, and Radioactive Waste, 18(3), 04014012.
    Huang, I.-S., & Zimba, P. V. (2019). Cyanobacterial bioactive metabolites—A review of their chemistry and biology. Harmful Algae, 86, 139–209.
    Jiang, J., Saint, C. P., Cane, D. E., & Monis, P. T. (2008). Isolation and characterization of the gene associated with geosmin production in cyanobacteria. Environmental Science & Technology, 42(21), 8027–8032.
    Johnson, A. C., & and Castenholz, R. W. (2000). Preliminary Observations of the Benthic Cyanobacteria of Waldo Lake and Their Potential Contribution to Lake Productivity. Lake and Reservoir Management, 16(1-2), 85–90. https://doi.org/10.1080/07438140009354225
    Johnson, A. C., & Castenholz, R. W. (2000). Preliminary observations of the benthic cyanobacteria of Waldo Lake and their potential contribution to lake productivity. Lake and Reservoir Management, 16(1-2), 85–90.
    Jüttner, F., & Watson, S. B. (2007). Biochemical and ecological control of geosmin and 2-methylisoborneol in source waters. Appl Environ Microbiol, 73(14), 4395–4406. https://doi.org/10.1128/aem.02250-06
    Kang, M., Kim, D.-W., Park, M., Kim, K., & Min, J.-H. (2023). Managing the Taste and Odor Compound 2-MIB in a River-Reservoir System, South Korea. Water, 15(23), 4107.
    Khalilzadeh Poshtegal, M., & Mirbagheri, S. A. (2023). Simulation and modelling of heavy metals and water quality parameters in the river. Scientific Reports, 13(1), 3020.
    Kim, C., Lee, S. I., Hwang, S., Cho, M., Kim, H.-S., & Noh, S. H. (2014). Removal of geosmin and 2-methylisoboneol (2-MIB) by membrane system combined with powdered activated carbon (PAC) for drinking water treatment. Journal of Water Process Engineering, 4, 91–98.
    Kim, T.-K., Moon, B.-R., Kim, T., Kim, M.-K., & Zoh, K.-D. (2016). Degradation mechanisms of geosmin and 2-MIB during UV photolysis and UV/chlorine reactions. Chemosphere, 162, 157–164.
    Koester, K. (2011). Measuring and modeling geosmin removal from Horsetooth Reservoir water by powdered activated carbon for selected contact times. Masters Abstracts International,
    Lee, J. E., Park, R., Yu, M., Byeon, M., & Kang, T. (2023). qPCR-based monitoring of 2-Methylisoborneol/Geosmin-producing cyanobacteria in drinking water reservoirs in South Korea. Microorganisms, 11(9), 2332.
    Lin, T.-F., Watson, S., & Suffet, I. M. (2018). Taste and odour in source and drinking water: Causes, controls, and consequences. IWA Publishing.
    Lin, T.-F., Wong, J.-Y., & Kao, H.-P. (2002). Correlation of musty odor and 2-MIB in two drinking water treatment plants in South Taiwan. Science of the Total Environment, 289(1-3), 225–235.
    Lin, T., Watson, S., Devesa, R., Bruchet, A., Burlingam, G., Dietrich, A., & Suffet, M. (2012). Off-flavours in the aquatic environment: a global issue. Global trends & challenges in water science, research and management-A compendium of hot topics and features from IWA Specialist Groups, 58–63.
    Lloyd, S. W., Lea, J. M., Zimba, P. V., & Grimm, C. C. (1998). Rapid analysis of geosmin and 2-methylisoborneol in water using solid phase micro extraction procedures. Water Research, 32(7), 2140–2146.
    Lu, K.-Y., Chiu, Y.-T., Burch, M., Senoro, D., & Lin, T.-F. (2019). A molecular-based method to estimate the risk associated with cyanotoxins and odor compounds in drinking water sources. Water Research, 164, 114938.
    Lundgren, B., Grimvall, A., & Sävenhed, R. (1988). Formation and removal of off-flavour compounds during ozonation and filtration through biologically active sand filters. Water Science and Technology, 20(8-9), 245–253.
    Ma, L., Wang, C., Li, H., Peng, F., & Yang, Z. (2018). Degradation of geosmin and 2-methylisoborneol in water with UV/chlorine: Influencing factors, reactive species, and possible pathways. Chemosphere, 211, 1166–1175.
    Matthijs, H. C., Visser, P. M., Reeze, B., Meeuse, J., Slot, P. C., Wijn, G., Talens, R., & Huisman, J. (2012). Selective suppression of harmful cyanobacteria in an entire lake with hydrogen peroxide. Water Research, 46(5), 1460–1472.
    Medsker, L. L., Jenkins, D., & Thomas, J. F. (1968). Odorous compounds in natural waters. An earthy-smelling compound associated with blue-green algae and actinomycetes. Environmental Science & Technology, 2(6), 461–464.
    Medsker, L. L., Jenkins, D., Thomas, J. F., & Koch, C. (1969). Odorous compounds in natural waters. 2-Exo-hydroxy-2-methylbornane, the major odorous compound produced by several actinomycetes. Environmental Science & Technology, 3(5), 476–477.
    Mendez, L., Mahdy, A., Ballesteros, M., & González-Fernández, C. (2015). Chlorella vulgaris vs cyanobacterial biomasses: Comparison in terms of biomass productivity and biogas yield. Energy conversion and management, 92, 137–142.
    Moorhead, D., Schmeling, J., & Hawes, I. (2005). Modelling the contribution of benthic microbial mats to net primary production in Lake Hoare, McMurdo Dry Valleys. Antarctic Science, 17(1), 33–45.
    Morton, K. W., & Mayers, D. F. (2005). Numerical solution of partial differential equations: an introduction. Cambridge university press.
    Mustapha, S., Tijani, J., Ndamitso, M., Abdulkareem, A., Shuaib, D., & Mohammed, A. (2021a). A critical review on geosmin and 2-methylisoborneol in water: sources, effects, detection, and removal techniques. Environmental monitoring and assessment, 193(4), 204.
    Mustapha, S., Tijani, J., Ndamitso, M., Abdulkareem, A., Shuaib, D., & Mohammed, A. (2021b). A critical review on geosmin and 2-methylisoborneol in water: sources, effects, detection, and removal techniques. Environmental Monitoring and Assessment, 193, 1–34.
    Neilan, B. A., Pearson, L. A., Muenchhoff, J., Moffitt, M. C., & Dittmann, E. (2013). Environmental conditions that influence toxin biosynthesis in cyanobacteria. Environmental microbiology, 15(5), 1239–1253.
    Nerenberg, R., Rittmann, B. E., & Soucie, W. J. (2000). Ozone/biofiltration for removing MIB and geosmin. Journal‐American Water Works Association, 92(12), 85–95.
    Paerl, H. W., & Paul, V. J. (2012). Climate change: Links to global expansion of harmful cyanobacteria. Water Research, 46(5), 1349–1363. https://doi.org/https://doi.org/10.1016/j.watres.2011.08.002
    Pang, M., Song, W., Liu, Y., & Pang, Y. (2021). Simulation of the parameters effecting the water quality evolution of Xuanwu Lake, China. International Journal of Environmental Research and Public Health, 18(11), 5757.
    Persson, P.-E. (1980). Sensory properties and analysis of two muddy odour compounds, geosmin and 2-methylisoborneol, in water and fish. Water Research, 14(8), 1113–1118.
    Persson, P. (1974). On flavour tainting of fish, with special reference to the Oulu sea area (Bothnian Bay). Rep. Nat. Bd Wat. Finl, 65, 1–262.
    Power, M. E. (1990). Benthic Turfs vs Floating Mats of Algae in River Food Webs. Oikos, 58(1), 67–79. https://doi.org/10.2307/3565362
    Power, M. E. (1990). Benthic turfs vs floating mats of algae in river food webs. Oikos, 67–79.
    Prieto-Barajas, C. M., Valencia-Cantero, E., & Santoyo, G. (2018). Microbial mat ecosystems: structure types, functional diversity, and biotechnological application. Electronic journal of biotechnology, 31, 48–56.
    Radha, R., Singh, R. K., & Singh, M. K. (2022). Contaminant transport analysis under non-linear sorption in a heterogeneous groundwater system. Applied Mathematics in Science and Engineering, 30(1), 736–761.
    Rehakova, K., Čapková, K., Konopáčová, E., Nedoma, J., Mareš, J., Bešta, T., Štenclová, L., & Kust, A. (2023). Unveiling the ecological significance of algal mats and meadows: Insights into phosphorus cycling and primary production of benthic algae in post-mining lakes. ARPHA Conference Abstracts,
    Ridal, J. J., Brownlee, B., & Lean, D. R. (1999). Occurrence of the odor compounds, 2-methylisoborneol and geosmin in eastern Lake Ontario and the upper St. Lawrence River. Journal of Great Lakes Research, 25(1), 198–204.
    Rider, Z., Percich, A., Hiripitiyage, Y., Harris, T. D., Sturm, B. S., Wilson, A. E., Pollock, E. D., Beaver, J. R., & Husic, A. (2024). Drivers of cyanotoxin and taste-and-odor compound presence within the benthic algae of human-disturbed rivers. Water Research, 253, 121357.
    Rosen, B. H. (2025). Color Atlas of Freshwater Algae.
    Sahay, R. R. (2013). Predicting Longitudinal Dispersion Coefficients in Sinuous Rivers by Genetic Algorithm. Journal of Hydrology and Hydromechanics, 61(3), 214–221. https://doi.org/https://doi.org/10.2478/johh-2013-0028
    Saltelli, A., Ratto, M., Andres, T., Campolongo, F., Cariboni, J., Gatelli, D., Saisana, M., & Tarantola, S. (2008). Global sensitivity analysis: the primer. John Wiley & Sons.
    Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature methods, 9(7), 671–675.
    Senavirathna, M. D. H. J., & Jayasekara, M. A. D. D. (2023). Temporal variation of 2‐MIB and geosmin production by Pseudanabaena galeata and Phormidium ambiguum exposed to high‐intensity light. Water Environment Research, 95(1), e10834.
    Senavirathna, M. D. H. J., & Jayasekara, M. A. D. D. (2025). Influence of Temperature Regimes on the Production of 2‐MIB and Geosmin by Pseudanabaena galeata and Phormidium ambiguum. Water Environment Research, 97(11), e70201.
    Shen, Q., Wang, Q., Miao, H., Shimada, M., Utsumi, M., Lei, Z., Zhang, Z., Nishimura, O., Asada, Y., & Fujimoto, N. (2022). Temperature affects growth, geosmin/2-methylisoborneol production, and gene expression in two cyanobacterial species. Environmental Science and Pollution Research, 29(8), 12017–12026.
    Streeter, H. W., & Phelps, E. B. (1925). A study of the pollution and natural purification of the Ohio River. United States Public Health Service.
    Su, M., Suruzzaman, M., Zhu, Y., Lu, J., Yu, J., Zhang, Y., & Yang, M. (2021). Ecological niche and in-situ control of MIB producers in source water. Journal of Environmental Sciences, 110, 119–128.
    Suffet, I., Braithwaite, S., Zhou, Y., Bruchet, A., Lin, T.-F., Watson, S., & Dietrich, A. M. (2019). The drinking water taste-and-odour wheel after 30 years. In: IWA Publishing, London, UK.
    Suffet, I. H., Khiari, D., & Bruchet, A. (1999). The drinking water taste and odor wheel for the millennium: Beyond geosmin and 2-methylisoborneol. Water Science and Technology, 40(6), 1–13. https://doi.org/https://doi.org/10.1016/S0273-1223(99)00531-4
    Suurnäkki, S., Gomez-Saez, G. V., Rantala-Ylinen, A., Jokela, J., Fewer, D. P., & Sivonen, K. (2015). Identification of geosmin and 2-methylisoborneol in cyanobacteria and molecular detection methods for the producers of these compounds. Water Research, 68, 56–66.
    Taylor, G. I. (1953). Dispersion of soluble matter in solvent flowing slowly through a tube. Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences, 219(1137), 186–203.
    Taylor, W., Losee, R. F., Torobin, M., Izaguirre, G., Sass, D., Khiari, D., & Atasi, K. (2006). Early warning and management of surface water taste-and-odor events.
    Thermo Fisher Scientific Inc Real-Time PCR (qPCR) Learning Center. https://www.thermofisher.com/tw/zt/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center.html
    Thomas, J. W. (2013). Numerical partial differential equations: finite difference methods (Vol. 22). Springer Science & Business Media.
    Thomas, L. S., & Gehrig, J. (2020). ImageJ/Fiji ROI 1-click tools for rapid manual image annotations and measurements. Micropublication Biology, 2020, 10.17912/micropub. biology. 000215.
    Thomas, M. (2021). Spatial and temporal investigation of taste and odor-producing microorganisms in Lake Saugahatchee Auburn University].
    Tsao, H.-W., Michinaka, A., Yen, H.-K., Giglio, S., Hobson, P., Monis, P., & Lin, T.-F. (2014). Monitoring of geosmin producing Anabaena circinalis using quantitative PCR. Water Research, 49, 416–425.
    Uwins, H., Teasdale, P., & Stratton, H. (2007). A case study investigating the occurrence of geosmin and 2-methylisoborneol (MIB) in the surface waters of the Hinze Dam, Gold Coast, Australia. Water Science and Technology, 55(5), 231–238.
    Vadeboncoeur, Y., Moore, M. V., Stewart, S. D., Chandra, S., Atkins, K. S., Baron, J. S., Bouma-Gregson, K., Brothers, S., Francoeur, S. N., & Genzoli, L. (2021). Blue waters, green bottoms: benthic filamentous algal blooms are an emerging threat to clear lakes worldwide. BioScience, 71(10), 1011–1027.
    Wang, H., Li, L., Cheng, S., Chen, L., Zhang, H., & Zhang, X. (2024). Production and release of 2-MIB in Pseudanabaena: Effects of growth phases on cell characteristics and 2-MIB yield. Ecotoxicology and Environmental Safety, 274, 116198.
    Wang, Q., Li, S., Jia, P., Qi, C., & Ding, F. (2013). A review of surface water quality models. The Scientific World Journal, 2013(1), 231768.
    Watson, S., & Jüttner, F. (2019). Biological production of taste and odour compounds. Taste and Odour in Source and Drinking Water: Causes, Controls, and Consequences, 63–112.
    Watson, S. B., Monis, P., Baker, P., & Giglio, S. (2016). Biochemistry and genetics of taste-and odor-producing cyanobacteria. Harmful Algae, 54, 112–127.
    Westerhoff, P., Rodriguez-Hernandez, M., Baker, L., & Sommerfeld, M. (2005). Seasonal occurrence and degradation of 2-methylisoborneol in water supply reservoirs. Water Research, 39(20), 4899–4912.
    Whitton, B., & Potts, M. (2002). The Ecology of Cyanobacteria: Their Diversity in Time and Space. https://doi.org/10.1007/0-306-46855-7
    Whitton, B. A., & Potts, M. (2007). The ecology of cyanobacteria: their diversity in time and space. Springer Science & Business Media.
    Wool, T. A., Ambrose Jr, R., & Martin, J. (2015). WASP7 Multiple Algae-Model Theory and User’s Guid. US EPA, Region, 4.
    World Meteorological Organization, W. (2024). State of Global Water Resources report 2023.
    World Meteorological Organization, W. (2025). State of Global Water Resources report 2024.
    Yih, S. M., & Davidson, B. (1975). Identification in nonlinear, distributed parameter water quality models. Water Resources Research, 11(5), 693–704.
    Young, W., Horth, H., Crane, R., Ogden, T., & Arnott, M. (1996). Taste and odour threshold concentrations of potential potable water contaminants. Water Research, 30(2), 331–340.
    Zhang, L., Huang, S., Peng, X., Liu, B., Zhang, X., Ge, F., Zhou, Q., & Wu, Z. (2021). Potential ecological implication of Cladophora oligoclora decomposition: Characteristics of nutrient migration, transformation, and response of bacterial community structure. Water Research, 190, 116741.
    Zheng, D., Wilén, B. M., Öberg, O., Wik, T., & Modin, O. (2024). Metagenomics reveal the potential for geosmin and 2‐methylisoborneol production across multiple bacterial phyla in recirculating aquaculture systems. Environmental microbiology, 26(10), e16696.
    交通部. (2018). 公路排水設計規範. In.
    地下水暨飲用水實驗室, 成. 常見藻類圖鑑.
    林, 財., 顏, 宏., 林, 秀., 邱, 宜., & 李, 紹. (2016). 公共給水有害藻類及代謝物監測與緊急應變處理技術之研究.
    林財富, 顏宏愷, 林秀蓮, 邱宜亭, & 李紹鈺. (2016). 公共給水有害藻類及代謝物監測與緊急應變處理技術之研究.
    張晴晴. (2025). 供水渠道中2-MIB的來源貢獻分析 國立成功大學]. 臺灣博碩士論文知識加值系統. 台南市. https://hdl.handle.net/11296/8kav65
    陳, 怡. (2023). 以分子生物技術分析底泥中產2-MIB藍綠菌及其菌種組成之研究 國立成功大學]. 臺灣博碩士論文知識加值系統. 台南市. https://hdl.handle.net/11296/2thehz
    陳, 意., 張, 俊., 林, 信., & 孫, 明. (2001). 不同材質灌溉排水渠道之研究. 水土保持學報, 67–80.
    童, 淑., 徐, 孟., 楊, 士., & 林, 財. (2002). 利用氧化劑去除原水中2-MIB之研究. 第二十七屆廢水處理技術研討會論文集.
    環境部. (2011). 環境影響評估河川水質模式評估技術規範.
    環境部. (2023). 2022年環境水質監測年報.
    環境部. (2026). 2025年環境水質監測年報.
    謝汶興. (2013). 2-MIB 與 Geosmin 在土壤-水系統分佈及淨水程序之處理研究

    QR CODE