本實驗主要探討膽固醇的添加對於雙鏈分子所構成之雙層膜的相行為與其特性的影響，採用小角度X光散射(Small Angle X-Ray Scattering, SAXS)以及磷31-核磁共振(31P-NMR)分別進行DTMA-DS (C12-C12)及DPPC (C16-C16)之雙層膜特性研究。
在DTMA-DS系統中，可以利用SAXS確認到層狀結構的形成，再進行膽固醇相反效應的探討。膽固醇的添加會使雙層膜相態呈現液晶有序相，故在凝膠相中的膽固醇添加，會使相態由原先單相凝膠相變成兩相共存再轉變為單一存在之液晶有序相，但在液晶相中的膽固醇添加，則因液晶相及液晶有序相二者流動性及結構相似，以致無法辨別。另外膽固醇相反效應皆會使雙層膜厚度增加。
在DPPC脂質體系統中，膽固醇的添加有添加穩定性的作用，此外在膽固醇濃度達20mol%後，隨著膽固醇濃度上升，除介面電位呈現下降趨勢外，粒徑、層數、接收光強度皆隨之上升。

This study mainly explores the effect of cholesterol on the phase behavior and characteristics of the bilayer membrane. It is composed of dialkyl chain molecules. By using small angle X-ray scattering (SAXS) and 31P-NMR, the bilayer characteristics of DTMA-DS (C12-C12) and DPPC (C16-C16) can be studied respectively.
In the DTMA-DS system, the formation of lamellar structure is confirmed by SAXS, then the opposite effect of cholesterol can be discussed. The addition of cholesterol would cause that the bilayer phase turn into liquid-ordered phase (Lo). Therefore, in gel phase (Lb), the disordering effect of cholesterol leads to the transformation on phase behavior which changes from the original gel phase (Lb) into two-phase coexistence (Lb+Lo) and then becomes a single phase, liquid-ordered phase(Lo). However, in liquid crystalline phase(La), because of the similar fluidity and structure between liquid crystalline phase(La) and liquid-ordered phase(Lo) so that the condensing effect of cholesterol doesn’t work effectively. In this case, SAXS can’t be used to distinguish the structural difference between the two phases (La and Lo) and identify. In addition, the opposite effect of cholesterol would both increase the bilayer thickness.
In the DPPC liposome system, the addition of cholesterol has the ability to enhance the stability of vesicles formation. In addition, when the cholesterol concentration is higher than 20mole%, with increasing cholesterol amounts, except zeta potential shows the decreased trend, the initial size, lamellarity, and the derived count rate all show increased trend.

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