中文摘要
　　此研究重點在於發展一個快速、簡便的生醫感測晶片，主要是應用在生物樣品的辨識研究上，我們結合了金奈米粒子當受質，因為它具有特殊的光學特性以及能提供較大的接觸表面等性質，我們希望能藉此來提高生物樣品的辨識靈敏度。
　　在這個實驗中，我們會先用化學修飾的方法將金奈米粒子單層固定在玻璃上，接著是利用一段有修飾FITC螢光染料的DNA，另一端修飾硫醇基，藉由硫醇基可與金奈米粒子形成共價鍵結 (covalent bond)，而使得DNA能固定在金奈米粒子上,這時FITC螢光染料會因為與金奈米粒子距離很近，而產生能量轉移的現象，因此會自行吸附到金奈米粒子的表面上(closed state)，以致螢光訊號會因此而被抑制住;當加入另一段互補的DNA時，會因為雜合作用(hybridization)而形成雙股DNA (double stranded)，促使FITC螢光染料從金奈米粒子表面釋放出來，螢光訊號也會隨之提昇，我們即可藉由這個訊號變化來辨識DNA之間的作用情形。
　　高效率的生醫感測晶片是具有廣大的市場需求，然而如何找出能提昇其靈敏度及應用領域的方法是非常重要的。而由此論文中所談的觀點提供了一種新的檢測方式，如此迅速、簡便的特性將會是未來發展生醫奈米科技的一種趨勢。

Abstract
　　This research is mainly base on developing a simple and convenient biosensing chip. It is used to identify different variety of biology samples. Gold nanoparticle, acting as a substrate, has a special optical characteristic and provides a larger contacting surface; therefore, it is combined with the biosensing chip in order to increase detection sensitivity.
　　In this experiment, we use chemical modification method to coat a single layer of gold nanoparticles onto the glass substrate. A piece of DNA that modifies FITC fluorescence on one end and thiol group on the other end is then used. Thiol group can bind with gold nanoparticles forming a covalent bond; consequently, the DNA is stabilized on the glass substrate via gold nanoparticles. Due to the fact that the gold nanoparticle is quite close to the FITC fluorescence, they tend to bind with each other, forming a direct energy transformation. This phenomenon is so called an assembled (closed) state. As a result, the fluorescent signal would fade out from lose of energy of the FITC fluorescence. When a single complementary DNA strand is introduced, a double stranded DNA would form via hybridization. Not only if the fluorescent signal is discharged through the gold nanoparticle, but it has also become a stronger signal. We can then use this advantage in order to recognize DNA interaction.
　　In the market, research and development of a high performance biosensing chip is very popular and demanding; therefore, it is very important to find out how to improve its sensitivity and apply the technology to various usages. In this thesis, an outlook is brought up which suggests that a new sensing method with quicker and more convenient advantages would be beneficial to the future!

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