肌酸酐 (creatinine) 為由肌肉中肌酸 (creatine) 分解而來的物質，為人體腎臟代謝的終端產物之一，是一種常用來評估腎功能的重要指標。本研究乃利用此分子模版技術 (molecular imprinting) 來製備對肌酸酐具有特殊辨識能力的分子模版高分子。製備方法是以溶膠-凝膠法 (sol-gel)，在前驅物四乙烷基矽烷 (tetraethoxy-silane，TEOS) 摻入有機功能性單體2-烴醯胺基-2-甲基-1-丙磺酸 (2-acrylamido-2-methyl- propane-sulfonic acid，AMPS)，經過水解-縮合反應後形成了所謂的有機-無機混成模版高分子。在單成份肌酸酐溶液吸附之下，有機-無機混成模版高分子對肌酸酐之鍵結容量為 32.57 ± 2.50 mg creatinine/g MIP，而非模版高分子對肌酸酐之鍵結容量則為 9.65 ± 1.47 mg creatinine/g NIP。兩者相除得到之模版因子 (imprinting factor) 為3.42 ± 0.37。除此之外，該有機-無機混成模版高分子在混合溶液吸附之下，對於肌酸酐也具有相當良好的辨識能力，對混合物肌酸、N-羥基丁二硫亞氨 (N-hydroxy- succinimide)、肌酸酐以及吡咯烷酮 (2-pyrro-lidinone) 之選擇比分別為0.053 ± 0.014、0.034 ± 0.008、0.854 ± 0.026以及0.059 ± 0.004。

　　在確認其特異性吸附之可行性後，以分子模印技術將肌酸酐辨識基座建置於電極上，配合以阻抗式感測法來分析肌酸酐濃度。初步測試結果顯示這種感測方式是可行的，但感測效果及實用性則待進一步評估。

　　Creatinine is produced from creatine in muscle and is one of the end products of kidney metabolism. It is usually used as an important index to estimate the kidney function. In this study the polymer with specific recognition ability for creatinine is synthesized via molecular imprinting technique. The preparation method involves the sol-gel process. The inorganic precursor, tetraethoxysilane (TEOS), is mixed with the organic functional monomer, 2-acrylamido-2-methyl-propanesulfonic acid (AMPS), through the hydrolysis-condensation reaction, the so-called organic-inorganic hybrid creatinine imprinted polymer is formed. The binding capacity of the polymer towards creatinine in single creatinine solution is 32.57 ± 2.50 mg creatinine/ g MIP and 9.65 ± 1.47 mg creatinine/g NIP from the non-imprinted polymer. The imprinting factor, defined as the binding capacity from the H-MIP to that of H-NIP, is 3.42 ± 0.37. On the other hand, the selectivity ratios in mixture solution comprised of creatine, N-hydroxysuccinimide, creatinine and 2-pyrro-lidinone were 0.053 ± 0.014, 0.034 ± 0.008, 0.854 ± 0.026 and 0.059 ± 0.004, respectively.

　　As the feasibility of this hybrid material for specific recognition of creatinine is confirmed, the creatinine-recognition polymer film is fabricated on the Au-electrode and is then analyzed via impedometric transducer. Such an electrode is feasible from the initial investigation, but further comfirmation on the sensing effect and the clinical application still need to investigate.

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