為控制水庫藍綠菌及其有害代謝物，本研究擬開發光催化材料，並探討其應用於水庫，控制藍綠菌及破壞藻毒素及臭味物質之可行性。
研究中首先探討二氧化鈦固定化技術，包括以旋轉塗佈法、水熱法、共濺鍍法等進行製備二氧化鈦薄膜，並以自由基生成、二氧化鈦釋出風險及材料特性分析等因素，決定最佳化之二氧化鈦薄膜。其後，並以最佳化之二氧化鈦薄膜，結合紫外光及過氧化氫，進行藍綠菌及藻毒素之光催化氧化試驗、及藍綠菌抑制生長試驗。研究中以銅綠微囊藻(Microcystis aeruginosa)為代表性藍綠菌，氧化實驗過程中，並分析細胞完整性、細胞活性、自由基濃度、過氧化氫殘餘濃度、微囊藻毒素濃度等，以掌握試驗結果。
研究結果顯示，以二氧化鈦薄膜、UV搭配使用，可成為一自由基殺藻系統，成功產生10^(-15)Ｍ之自由基濃度，長期作用下對藻體進行破壞，然自由基濃度卻不足以在數小時內降解釋出之微囊藻毒素。若以過氧化氫與二氧化鈦薄膜/UVA或與UVA搭配使用，則呈現較佳之細胞破壞效果，且能有效降解微囊藻毒素，但二氧化鈦薄膜/過氧化氫/UV系統有過氧化氫大量消耗的現象，其一階降解常數約為無二氧化鈦薄膜催化時之8至9倍，進而使氫氧自由基產量驟降，顯示過氧化氫之消耗對本系統影響甚鉅。若控制二氧化鈦薄膜之放入時間與藻毒素大量釋出之時間重疊，對藻毒降解實驗效果更佳，顯示改變薄膜投入時間，能加強目標污染物之降解。
微囊藻抑制生長實驗顯示，系統中可產生低濃度(~10^(-16) Ｍ)氫氧自由基，系統在十六天內對微囊藻生長具抑制作用，並可使微囊藻毒降至世界衛生組織之建議值以下。該實驗亦發現微囊藻長期於UVA光照下，生長亦會受影響，比生長速率僅為控制組之1/3，其原因待後續進一部探討。
研究中藉Delayed Chick Watson Model及Delayed Hom Model兩個動力模式，分離過氧化氫與氫氧自由基對細胞破裂的影響；並以上述模式及藻毒素二階降解模式，模擬氧化試驗系統中藻細胞破裂及毒素破壞行為，結果顯示該二模式可有效模擬細胞破裂、及後續的總微囊藻毒素降解，可作為後續二氧化鈦薄膜/過氧化氫/UVA系統應用之參考。

To control the cyanobacteria and their metabolites in source water, in this study titanium dioxide (TiO2) thin film and hydrogen peroxide (H2O2) were combined with UV-A illumination were examined for their effect on cell integrity and metabolites degradation. In first part, TiO2 thin films were first synthesized using three approaches, including spin coating, hydrothermal, and co-sputtering methods. The best TiO2 thin film synthesized, spin coating at 1500 rpm (SC1500), was obtained based on high hydroxyl radical production and low nanoparticle release. The SC1500 was then analyzed surface properties and used in the batch oxidation and batch incubation experiments for cyanobacteria control.
To understand the effect of hydroxyl radical, H2O2, and UV-A light on cell integrity and microcystins (MCs), oxidation experiments were conducted, using Microcystis aeruginosa as the studied cyanobacterium. In the experiments, cell integrity, hydroxyl radical concentration, residual hydrogen peroxide, MCs concentration were measured, and cell number and cell activity are additionally analyzed in incubation experiments.
The results showed that exposure to long-term hydroxyl radical, for the TiO2 thin film/UV-A system and average radical concentration = 110-15 M, the integrity of
Microcystis cells were impacted. However, the MCs released from the ruptured cells were not degraded. For the system with H2O2, TiO2 thin film and UV-A, the cells were ruptured and the released MCs were more efficiently degraded by radical produced, if compared to the system without addition of H2O2. In addition, adjusting the placing time of TiO2 thin film into the H2O2/UV-A system might enhance the degradation of MCs. Nevertheless, the consumption of H2O2 which resulted in low H2O2 concentration and low hydroxyl radical production, might slow down the reaction rate with Microcystis cells and MCs. For incubation test, it showed low concentration of hydroxyl radical (TiO2 thin film/UV-A system, 10-16M) can inactivate the Microcystis in 16 days, maintaining the MCs concentration to below the guideline value in drinking water announced by WHO. In addition, UV-A irradiance may also inhibit the growth of Microcystis.
Two dual-oxidant kinetic models, including Delayed Chick-Watson Model and Delayed Hom model, were developed and successfully simulated the experimental data of cell integrity. The rate constants between Microcystis cells and H2O2 as well as hydroxyl radical in TiO2 thin film/H2O2/UV-A system were successfully determined by the developed model. The kinetic models for cell rupture were further combined with MCs degradation model to predict the change of MCs concentration over time, and reasonable fits were found between the models and data. The developed models and extracted rate constants may be used to predict cell rupture and MCs degradation when applying TiO2 thin film and H2O2 in natural water for the control the cyanobacteria and metabolites.

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