酸水解木質纖維素是一種快速且有效獲得還原糖的方法，但是水解液中的酸必須回收，以降低生產的成本及避免對環境造成汙染。本研究利用電透析裝置能夠有效地回收芒草 (Miscanthus floridulus) 水解液中的鹽酸。在水解的程序，於芒草與鹽酸重量比1/5，以1%(w/w)鹽酸於溫度121 oC，壓力1.2 atm 下水解1 小時，可獲得最高之還原糖濃度70 g/L。在分離還原糖與鹽酸的程序，於施加電壓5 V/cm 及處理時間20 分鐘之條件下，可得鹽酸移除率為90%。芒草水解液經電透析處理、調整pH 值及添加微量元素後，以Pseudomonas aeruginosa S2 利用水解液中之還原糖生產鼠李醣脂，其產率約208 g/L。實驗結果顯示，電透析程序可以有效地回收鹽酸，且水解液經電透析處理後可以被Pseudomonas aeruginosa S2 利用生產鼠李醣脂。

Acid hydrolysis of cellulose to obtain sugar is an effective approach but the recovery of acid for reusage is necessary to develop for reducing cost and preventing pollution. An efficient process for separation of acid and sugar from hydrolyzed Miscanthus floridulus with diluted hydrochloric acid was developed. The highest sugar concentration was 70 g/L when hydrolysis was carried out with 1% of hydrochloric acid at 121 oC for 60 min and Miscanthus floridulus to acid ratio was 1:5. Separation of acid from the sugar solution was conducted through electrodialysis. In order to evaluate the performance of electrodialysis process, the effects of applied voltage and hydraulic retention time (HRT) on acid recovery were investigated. The results showed that efficiency of separation reaches 90% at the optimal conditions of applied
voltage 5 V/cm for 20 min. After electrodialysis process and pH adjustment, the sugar solution was subjected to fermentation using Pseudomonas aeruginosa S2. The rhamnolipid yield was approximately 208 mg/L. These experimental results indicate that the electrodialysis is an effective and important method to recover acid from the sugar solution and remove the toxic compound for Pseudomonas aeruginosa S2.

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