文心蘭(Oncidium spp.)為蘭科(Orchidaceae)文心蘭亞族(Oncidiinae)文心蘭屬，在傳統醫藥方面常用於治療肌肉挫傷、發炎、傷口、外部出血和皮膚乾裂等，並在臨床研究中顯示具有抗菌、刺激傷口癒合、抗原蟲、抗癌、抗氧化、血管舒張等活性，然而，關於檸檬綠的化學成分分析研究有限，目前已證實文心蘭亞族植物內化合物主要為 phenanthrene 類，其次為 flavonoid，bibenzyl，steroid 及 phenolic 類化合物，且在假球莖早期生長階段含有豐富的多醣，花瓣中含有類胡蘿蔔素和花青素等色素。因此，本研究旨在進行檸檬綠的化學成分分析，並探討檸檬綠的生物活性。
將檸檬綠假球莖與根部之酒精萃取物進行溶液極性分劃，假球莖酒精萃取物得到正丁醇層與水層，根部酒精萃取物得到氯仿層、正丁醇層與水層，後續利用各種層析技術進行天然物分離。分離所得之化合物使用核磁共振、質譜儀、紫外-可見光光譜儀與紅外線光譜儀之光譜進行結構鑑定。本研究共分離得三十個化合物，包括 10 個phenanthrene 成分、5 個 bibenzyl 成分、3 個 flavonoid 成分、3 個 phenol 成分、1 個nucleotide 成分、3 個 carbohydrate 成分、3 個 lipid 成分、1 個 organic acid 成分及 1 個terephthalate 成分，其中 5-hydroxy-1,2,3,4-tetramethoxyphenanthrene 為為首次報導的天然化合物。在藥理活性方面，假球莖之酒精萃取物、正丁醇層與水層進行一系列的生物活性實驗，結果顯示假球莖之酒精萃取物、正丁醇層與水層皆具抗登革及抗發炎活性。

Oncidium spp. Honey Angel belonging to the Oncidium genus of Orchidaceae. In traditional medicine, commonly used to treat muscle contusion, inflammation, wound, external bleeding and chapped skin. Clinical studies have shown it to have antimicrobial, wound healing stimulating, antiprotozoal, anticarcinogenic, antioxidant and vasodilatory activities. However, the chemical components of Oncidium spp. Honey Angel are rarely studied. Prior investigations have unveiled polysaccharide-rich mucilage within pseudobulbs; phenanthrenes, flavonoids, bibenzyls and phenolic compounds in the entire plant; and pigments such as carotenoids and anthocyanins in its flowers. This investigation aimed to furnish comprehensive insights into the chemical composition from Oncidium spp. Honey Angel, thereby contributing to the understanding of the chemical taxonomy of Oncidium species.
The dried pseudobulbs and roots of Oncidium spp. Honey Angel were powdered and extracted with 95% ethanol to yield dried extracts. The resulting extracts were partitioned and separated through different column chromatographies. All the chemical structures of these isolated compounds were elucidated through NMR, UV, IR and mass spectrometry analyses. A series of bioactivity tests were conducted on the ethanol extract, n-butanol layer and water layer of the pseudobulbs, and the results showed they all have anti-dengue amd anti-inflammatory activities. So far, we have isolated thirty compounds, including ten phenanthrenes, five bibenzyls, three flavonoids, three phenols, one nucleotide, three carbohydrates, three lipids, one organic acid and one terephthalate, of which 5-hydroxy-1,2,3,4-tetramethoxyphenanthrene is the first reported natural compound. Subsequently, the isolated compounds will undergo further testing for anti-inflammatory and antioxidant activities.

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