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研究生： 胡曉晴
Hu, Hsiao-Ching
論文名稱： 藉由矽烷微奈米表面形貌引導聚電解質選擇性沉積
Selective Deposition of Polyelectrolyte via Silane Nanotopography-Mediated Process
指導教授： 李介仁
Li, Jie-Ren
學位類別： 碩士
Master
系所名稱： 理學院 - 化學系
Department of Chemistry
論文出版年： 2020
畢業學年度： 108
語文別： 中文
論文頁數： 109
中文關鍵詞： 聚電解質 、逐層沉積技術 、自組裝
外文關鍵詞： polyelectrolyte, layer-by-layer, self-assembly
相關次數： 點閱：106  下載：0 
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    • 伴隨組織工程技術的發展，研究人員開始能夠在體外製作出與體內組織及器官相似的層狀結構。在製作分層結構的方法中，逐層沉積技術已成為一種最常見且簡單的方式，它是一種將大分子沉積在介面上的方法，除了沉積在平面基板上，也可以在結構化的基板上選擇性的製作出層狀複合膜。其實驗步驟是將基材反覆浸泡在帶相反電荷的聚電解質溶液中，使其主要透過靜電相互作用構建聚電解質多層膜。本研究先是利用一些簡單、快速的方法製作出具微奈米結構的基板，再使用聚丙烯酸(PAA)與聚乙烯亞胺(PEI)這兩種聚電解質以逐層沉積的方式選擇性地自組裝於表面結構，形成聚電解質多層膜，進一步研究其表面形貌對細胞外基質(ECM)蛋白質吸附行為的影響。實驗中所使用的ECM蛋白為第一型膠原蛋白，它是眾多細胞外間質中含量最豐富的蛋白質且已被應用於製作生物材料，例如水凝膠和聚電解質多層膜。
    本研究證實了我們利用矽烷製作出了具微奈米結構的表面，並透過改變表面的幾何結構及官能基來模擬體內複雜的結構，此表面的結構能夠引導聚電解質的自組裝，結構化的聚電解質多層膜亦會再影響第一型膠原蛋白在此介面進行選擇性吸附。這個由聚電解質與第一型膠原蛋白所形成的仿生介面對於發展組織工程支架結構及促進細胞生長、分化皆具極大的潛力。

    The ability to generate hierarchical tissue structures in vitro might one day allow researchers to recreate truly biomimetic tissues in a dish. Among the methods used to produce hierarchical structures, layer-by-layer (LBL) assembly is a simple method to deposit macromolecules on interfaces. Based on immersing the substrate in oppositely charged polyelectrolyte (PE) solutions, multilayered films can be built through electrostatic interactions. In this research, we developed the LBL polyelectrolyte multilayer (PEM) composed of polyacrylic acid (PAA) and polyethylenimine (PEI) assembly on silane nanotopographies to investigate the influence of topographical cues on extracellular matrix (ECM) protein adsorption behavior. The ECM protein used for experiments is type I collagen which is the most abundant ECM protein and has been utilized to produce biomaterial systems, such as hydrogels and PEM films mainly. Our results demonstrate that PE assembly can be controlled by using different silane nanotopographies, forming biomimetic interfaces with various geometries. The spatial organization of silane nanotopography not only guides self-assembly of PE but also affects the exposure of their binding sites to collagen. The nanotopographies combined with PAA and PEI layers exhibit superior control in collagen adsorption to form biomimetic interfaces, offering great potential to trigger cell growth or differentiation on surfaces of tissue engineering scaffolds.

    目錄 IX 圖表目錄 XI 第一章 、緒論 1 1.1 前言 1 1.2 聚電解質(Polyelectrolyte, PE) 1 1.3 聚電解質微奈米製程 3 1.4 第一型膠原蛋白(Collagen type I, Col I) 6 1.5 原子力顯微鏡(Atomic Force Microscopy, AFM) 7 第二章 、探討聚電解質於單一成份矽烷微奈米結構之自組裝行為 10 2.1 前言 10 2.2 實驗部分 11 2.2.1 實驗材料 11 2.2.2 儀器 12 2.2.3 藥品配製 13 2.2.4 實驗步驟 14 2.3 結果與討論 17 2.3.1 聚電解質於一般純矽烷薄膜表面之型態 17 2.3.2 聚電解質於線狀矽烷微奈米結構自組裝之型態 20 2.3.3 電解質於蜂巢狀矽烷奈米結構自組裝之型態 28 2.3.4 矽烷結構高度對聚電解質自組裝的影響 30 2.3.5 聚電解質分子量對其於矽烷微奈米結構自組裝的影響 35 2.3.6 聚電解質分子結構對其於矽烷微奈米結構自組裝的影響 38 2.3.7 基材平整度對聚電解質排列的影響 41 2.3.8 聚電解質微奈米結構穩定性之探討 46 2.3.9 溫溼度對於聚電解質微奈米結構之影響 48 第三章 、探討聚電解質於多重成份矽烷微奈米結構之自組裝行為 50 3.1 前言 50 3.2 實驗部分 51 3.2.1 實驗材料 51 3.2.2 儀器 52 3.2.3 藥品配製 53 3.2.4 實驗步驟 54 3.3 結果與討論 57 3.3.1 具雙重官能基之線狀矽烷微奈米結構對聚電解質自組裝的影響 57 3.3.2 聚電解質於點狀矽烷微奈米結構自組裝之型態 65 3.3.3 聚電解質分子量對其於雙成份矽烷微奈米結構自組裝的影響 72 3.3.4 具三成份官能基之點線交錯矽烷微奈米結構對聚電解質自組裝的影響 76 第四章 、聚電解質微奈米結構應用於膠原蛋白之選擇性吸附 80 4.1 實驗部分 80 4.1.1 實驗材料 80 4.1.2 儀器 82 4.1.3 藥品配製 83 4.1.4 實驗步驟 84 4.2 結果與討論 86 4.2.1 膠原蛋白直接吸附於聚電解質薄膜 86 4.2.2 轉移膠原蛋白於聚電解質薄膜 95 4.3 結論 105 參考文獻 106

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