漫反射光譜學(Diffuse Reflectance Spectroscopy， DRS) 基礎原理是利用光擴散理論(photon diffusion theory)將量測得到的漫反射光譜計算轉換為組織的吸收係數與散射係數，不過，由於光子擴散理論的限制，當光源與偵測器的距離小於五倍的平均自由路徑長(1/(μ_a+μ_s'))時，光子經過組織並沒有經過足夠的散射及被偵測器收到，如此實際狀況將不滿足理論模型的邊界條件，導致光子擴散理論不適用，因此本研究所使用的理論模型為使用蒙地卡羅法建立核心的理論模型數據庫，再訓練類神經網路(Artificial Neural Network ，ANN)建立演算法。
為了避免新生兒抽血造成的感染風險，以及經皮量測上易受膚色影響的問題，本研究利用空間解析DRS，建構出一套非侵入式地量測系統，我們利用涵蓋波長400-600nm的光源，量測並計算新生兒皮膚的光學參數，在我們所量測的吸收光譜範圍內，組織的主要色團為帶氧血紅蛋白、不帶氧血紅蛋白、膽紅素與黑色素，藉由考慮黑色素在光譜中的貢獻來排除經皮量測易受膚色所干擾的影響。
本研究將會做三個階段的分析，第一階段為利用仿人體假體來驗證系統量測的可行性，第二階段利用實際照相進行人體皮膚影像處理，並與DRS系統量測並計算人體黑色素濃度，藉此驗證本系統可以將黑色素干擾所排除，第三階段為將收取臨床上新生兒的漫反射光譜，共會量測12個新生兒，共會量測身體上的二個部位(胸骨和左腳底板)，此二者為現今經皮量測或脈博血氧儀常見的量測位置，並與抽血檢驗做相關性的比較，藉此驗證本系統在臨床上能夠提供一診斷新生兒黃疸的依據。

In this study, we use spatially resolved diffuse reflectance spectroscopy (SRDRS) to construct a non-invasive measurement systems with the novel spectral analysis algorithm in order to measure and calculate the optical parameters of the neonatal skin. Within the range of the absorbance spectrum we measured, the main chromophores in human skin are hemoglobin, bilirubin and melanin. By taking melanin into consideration, the effects of skin color on transcutaneous measurement results are eliminated. This study will be conducted in three section of analysis. The first is to verify the feasibility of the system measurement using gelatin phantoms. Second, by processing human skin image and using DRS system to measure and calculate the human skin melanin concentration, we compared the results and verified that the DRS system could exclude melanin interference. Last, we measured 12 newborn neonates diffuse reflectance spectra including their sternum and the left sole, and are compared with total serum bilirubin (TSB) from blood tests. There was a high correlation between the bilirubin values we measured and TSB values in both positions: sternum (r = 0.93) and the left sole (r = 0.94).

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