隨著全球人口成長與都市化快速發展，水資源短缺與廢水污染已成為嚴峻的環境問題。自營營養型微藻（如藍綠菌）提供一項永續的解決方案，能夠利用廢水中的營養成分（如氨鹽、磷酸鹽與微量金屬），同時固定二氧化碳（CO₂），並產生具經濟價值的副產物，如藻藍蛋白（phycocyanin）。因應肉品需求上升，畜牧業產生了大量富含營養的廢水，若未妥善處理，將對環境構成重大威脅。同樣地，生活污水亦含有大量營養物質。將生活污水與養豬廢水混合處理，不僅可降低毒性、平衡營養比例，亦有助於提升微藻的生長環境。
本研究探討將此類混合廢水作為培養基，用於培養耐熱鹼性的藍綠菌Thermosynechococcus sp. CL-1（TCL-1）。該菌株源自台灣金崙溫泉，具備良好的環境適應性。研究中將養豬廢水分別以去離子水與生活污水進行20倍與25倍稀釋，並添加113.2 mM的溶解性無機碳作為碳源，於平板式光生物反應器（FPBR）中進行培養。初始生物量設為3.0 g/L，氨氮濃度介於50–80 mg/L之間。
本研究評估 TCL-1 在不同稀釋策略下的生物量增長、碳固定能力、氮去除效率與藻藍蛋白產量，藉此驗證結合微藻培養與混合生活污水與養豬廢水處理在資源永續利用上的潛力。研究結果顯示，在以生活污水將養豬廢水稀釋25倍的條件下，TCL-1 的最佳生物量生產力與CO₂固定速率分別達到85.0 ± 12.4 mg/L/h與155.4 mg/L/h。

With global population growth and rapid urbanization, water scarcity and wastewater pollution have become critical environmental concerns. Autotrophic microalgae, such as cyanobacteria, offer a sustainable solution by utilizing nutrients like ammonium, phosphate, and trace metals from wastewater while fixing CO₂ and producing valuable by-products such as phycocyanin.
Swine farming, driven by rising meat demand, generates nutrient-rich wastewater that poses environmental risks if untreated. Similarly, domestic sewage contains significant nutrient loads. Mixing domestic sewage with swine wastewater can reduce toxicity, balance nutrient ratios, and improve conditions for microalgal growth. This study evaluates the potential of using such mixed wastewater as a medium for cultivating Thermosynechococcus sp. CL-1 (TCL-1), a thermotolerant, alkaliphilic cyanobacterium isolated from Chinlun Hot Spring, Taiwan.
Swine wastewater was diluted 20 and 25 times with either DI water or domestic sewage. A carbon source of 113.2 mM dissolved inorganic carbon (DIC) was added, and cultivation was performed in a flat-panel photobioreactor (FPBR). Initial biomass was set at 3.0 g/L, with ammonium concentrations between 50–80 mg/L.
This study evaluates the performance of TCL-1 in terms of biomass growth, carbon sequestration, nitrogen removal, and phycocyanin production under various dilution strategies. The findings aim to demonstrate the potential of coupling microalgal cultivation with mixed domestic and swine wastewater treatment for sustainable resource utilization. The results show that the optimal biomass productivity and CO₂ fixation rate in mixed wastewater cultivation are 85.0 ± 12.4 mg/L/h and 155.4 mg/L/h, respectively, under the condition where swine wastewater was diluted 25 times using domestic sewage.

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