本研究以十六烷基三甲基溴化銨（hexadecyltrimethylammonium bromide, HTMAB）及十六烷基硫酸鈉（sodium hexadecylsulfate, SHS）所形成之離子對雙親分子（ion pair amphiphile, IPA）HTMA-HS為主材料，添加雙碳氫鏈二甲基溴化銨（dialkyldimethylammonium bromide, DXDAB）及膽固醇（cholesterol），以強制性程序製備出陰陽離子液胞（catanionic vesicle），並藉由改變組成探討分子碳鏈長度對於液胞之物理特性的影響。
實驗結果顯示，隨著膽固醇或DXDAB添加比率的提高，混合HTMA-HS/DXDAB/膽固醇所形成之液胞的穩定天數呈現增加的趨勢，表示此兩種添加劑能有效提升液胞之物理穩定性。進一步分析液胞的物理特性，顯示膽固醇的插入能促進液胞表面帶電特性，且提升液胞組成分子間的凡得瓦作用，而能顯著抑制液胞之聚集/融合。同時發現添加膽固醇會使液胞組成分子的碳氫鏈趨於不規則的排列，進一步推測是由於膽固醇對分子碳氫鏈之不同區段有不同的影響。DXDAB的添加能使液胞明顯帶正電，而增加液胞間的靜電排斥作用。然而在固定膽固醇添加比率的情況下，改變HTMA-HS與DXDAB的混合比例，液胞之界面電位並不隨DXDAB添加比率的提升而顯著增加。推測當添加少量DXDAB時，DXDA+易取代HTMA+而與HS-結合，促使HTMA+溶解至水相中，造成液胞之表面帶電特性隨時間下降，而導致物理穩定性的降低。
在添加膽固醇的情況下，比較不同碳鏈長度的IPA（HTMA-HS和HTMA-DS）與不同碳鏈長度的DXDAB混合所形成之液胞的物理特性，發現以HTMA-DS為主材料之液胞易形成鬆散的雙層膜結構，這應是由於分子本身碳鏈的不對稱，以及液胞組成分子間碳氫鏈的不匹配所致。膽固醇的插入則可撐開帶電分子間的距離，造成界面電位大幅提升而形成穩定的液胞。而由HTMA-HS形成之液胞系統具分子間作用強且堅硬的雙層膜結構，膽固醇則有提升界面電位和增強分子間作用的效果，因而能增進液胞之物理穩定性。由兩種IPA所形成之液胞均發現，含DHDAB之液胞的物理穩定性最佳，顯示提升液胞組成分子的總碳數與分子間碳鏈的匹配性能影響雙層膜結構並進一步達到穩定液胞的效果。

This study investigated the effects of vesicle-forming molecules on the physical properties of catanionic vesicles prepared by ion pair amphiphile (hexadecyltrimethylammonium-hexadecylsulfate, HTMA-HS), dialkyldimethylammonium bromide (DXDAB), and cholesterol through the forced formation process. The physical properties of vesicles included size, zeta potential, and physical stability. Moreover, the packing of hydrocarbon chains of vesicle-forming molecules was investigated by FT-IR. When the chain length of vesicle-forming molecules was longer, the presence of stronger molecular interaction was detected. Besides, the good structure match between vesicle-forming molecules would be helpful to form ordered bilayer structures. Therefore, the vesicles containing DHDAB or HTMA-HS could be stable.

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