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研究生：	許晉維 Hsu, Jin-Wei
論文名稱：	多孔性硫酸鈣性質研究 Investigation of properties of porous calcium sulfate
指導教授：	陳瑾惠 Chern Lin, Jiin-Huey 朱建平 Ju, Chien-Ping
學位類別：	碩士 Master
系所名稱：	工學院 - 材料科學及工程學系 Department of Materials Science and Engineering
論文出版年：	2008
畢業學年度：	96
語文別：	中文
論文頁數：	119
中文關鍵詞：	多孔性、硫酸鈣、支架
外文關鍵詞：	scaffolds, Calcium Sulfate, porous
相關次數：	點閱：133 下載：0
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骨組織工程是利用多孔性支架來運載細胞、基因和蛋白質等生物因子而使骨再生，多孔性支架必須要具有兩項功能才能夠確保骨缺陷可以成功地被治癒，多孔性支架必須要擁有足夠的孔隙度才能夠運載生物因子，並且在植入時必須要能夠提供暫時性的強度。
硫酸鈣具有良好的生物相容性以及引骨性，硫酸鈣不貴且被運用在填補各種骨缺陷是暨安全又方便的，而多孔性硫酸鈣的缺點是強度比較低，本實驗在於探討如何增加多孔性硫酸鈣的強度。
本研究是利用鹽溶-瀝出法(salt leaching)製造硫酸鈣支架，此硫酸鈣支架的強度約為5MPa，然後再將此硫酸鈣支架先浸泡C 溶液，再浸泡G 溶液，希望藉此形成H 的薄膜，並且增加硫酸鈣支架的強度。

Bone tissue engineering utilizes porous biomaterial scaffolds to deliver biofactors including cells, genes, and proteins to regenerate bone. Porous biomaterial scaffolds must fulfill two primary functions to ensure successful treatment of bone defect. The scaffold must have enough porosity in order to deliver biofactors and provide temporary mechanical support.
Calcium sulfate has superior biocompatibility and osteoconductivity. It is inexpensive and can be used safely and conveniently in a wide variety of bone defects. But the drawback of porous calcium sulfate is its low strength. This study is about how to increase its strength.
This study uses salt-leaching to produce porous calcium sulfate and its compressive strength is about 5MPa. And then this porous scaffold is successively immersed in C solution and G solution in order to form H thin film and increase porous scaffold's compressive strength.

總目錄
中文摘要.................................................Ⅰ
Abstract.................................................Ⅱ
致謝.....................................................Ⅲ
總目錄...................................................Ⅳ
表目錄...................................................Ⅷ
圖目錄...................................................Ⅸ
第一章 總序論............................................1
1-1 前言.................................................2
1-2 生醫材料的定義	.......................................2
1-3 生醫材料的發展	.......................................3
1-4 生醫材料的分類	.......................................6
1-5 生醫陶瓷的種類	......................................13
1-6 生醫材料的性質要求..................................18
1-6-1生醫材料的一般性要求...............................18
1-6-2骨科植入材在力學上的要求...........................19
1-6-3骨科植入材的生物學要求.............................19
1-7人體骨組織結構成分及性質簡介.........................21
1-7-1 人體骨骼之功能....................................21
1-7-2 人體骨骼之化學成分................................21
1-7-3 骨骼的構成........................................22
1-7-4 骨骼細胞及其功能..................................23
1-7-5 骨骼的創傷癒合....................................23
第二章 基礎理論與文獻回顧...............................29
2-1 醫用硫酸鈣的發展....................................30
2-2 硫酸鈣的基本性質....................................37
2-2-1 α型半水硫酸鈣和β型半水硫酸鈣......................37
2-2-2 硫酸鈣的水合硬化過程..............................41
2-2-3添加劑對硫酸鈣水合反應的影響.......................42
2-3 生物支架............................................43
2-4 生物支架的基本性質..................................43
2-5支架製備方法.........................................48
第三章 實驗步驟與方法...................................53
3-1實驗使用之藥品.......................................54
3-1-1基底材料...........................................54
3-1-2硬化劑.............................................54
3-1-3填充材料...........................................54
3-1-4浸泡溶液...........................................54
3-2 實驗步驟............................................56
3-2-1 多孔性硫酸鈣支架的製作及磷酸鈣薄膜的形成..........56
3-2-2 提升多孔性硫酸鈣支架的孔隙度......................56
3-2-3 複合材支架的製作..................................57
3-3實驗原理.............................................61
3-3-1 抗壓強度測試	......................................61
3-3-2 掃描式電子顯微鏡(Scanning Electron Microscopy, SEM)分析......................................................61
3-3-3 X光繞射(X-ray diffraction, XRD)分析...............62
3-3-4 pH值量測..........................................63
3-3-5 孔隙度測試(Porosity)..............................64
3-3-6 細胞毒性測試(Cytotoxicity Assay)..................65
第四章 結果與討論.......................................69
4-1多孔性硫酸鈣支架的製作及磷酸鈣薄膜的形成.............70
4-1-1 抗壓強度測試	......................................70
4-1-2 孔隙度測試........................................73
4-1-3  SEM顯微結構觀察..................................75
4-1-4  XRD分析..........................................76
4-1-5  EDS分析..........................................76
4-1-6  pH值量測.........................................77
4-1-7 細胞毒性測試......................................78
4-2 提升多孔性硫酸鈣支架的孔隙度.......................101
4-2-1 抗壓強度測試.....................................101
4-2-2 XRD分析..........................................101
4-2-3 抗壓強度測試.....................................101
4-2-4 孔隙度測試.......................................102
4-3 複合材支架的製作...................................105
4-3-1 抗壓強度測試.....................................105
4-3-2 XRD分析..........................................105
4-3-3 EDS分析..........................................106
4-3-4  SEM顯微結構觀察.................................106
第五章 總結論..........................................112
參考文獻...............................................115
                                    

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