本研究藉由表面壓－面積及表面電位－面積等溫線、鬆弛曲線和熱力學分析，探討添加雙十六碳鏈磷酸鹽（dihexadecyl phosphate, DHDP）及膽固醇對於不同碳鏈長度之離子對雙親分子（ion pair amphiphile, IPA），hexadecyltrimethylammonium-hexadecylsulfate（HTMA-HS）和hexadecyltrimethylammonium-dodecylsulfate（HTMA-DS）Langmuir單分子層行為的影響。
對兩種IPA/DHDP混合單分子層而言，等溫線與熱力學分析顯示DHDP具有與膽固醇相似的凝縮效應（condensing effect），而此效應在HTMA-DS/DHDP混合單分子層中較為顯著。由單分子層鬆弛實驗可知，隨著DHDP添加量的增加，混合單分子層在氣/液界面上的穩定性越高。在兩種IPA/膽固醇混合單分子層中，由等溫線可知，當IPA單分子層處於排列鬆散的狀態下，膽固醇的凝縮效應相當明顯，但若IPA單分子層中分子的排列較緊密，其效應則不顯著，甚至使得混合單分子層的凝縮相出現相轉行為。但整體來說，膽固醇的添加有助於增加兩種IPA/膽固醇混合單分子層的分子間交互作用。在DHDP/膽固醇混合單分子層中，由熱力學分析可知，DHDP和膽固醇分子間的交互作用略大於DHDP與膽固醇自身分子間的作用，在此混合單分子層系統中亦在凝縮相發現相轉行為。由鬆弛實驗可知，膽固醇的添加因發生成核成長現象，使之無助甚至破壞兩種IPA或DHDP單分子層在表面壓為30 mN/m時的穩定性。
就添加膽固醇對兩種IPA/DHDP混合單分子層行為的影響而言，膽固醇的添加會使得凝縮單分子層於高表面壓時發生重組行為，並造成分子排列較緊密的混合單分子層中的分子碳氫鏈傾向於非直立排列，但對於分子排列較鬆散的混合單分子層中的分子碳氫鏈傾向於直立排列。整體而言，膽固醇的添加會增加兩種IPA/DHDP混合單分子層的分子間作用。由鬆弛行為可知，在表面壓30 mN/m的狀態下，膽固醇的添加皆使得兩種IPA/DHDP混合單分子層的穩定性下降。
若將三成分系統之混合單分子層的分析結果與相同比例材料所製備出之陰陽離子型液胞（catanionic vesicle）的穩定天數比較，當DHDP或膽固醇的含量增加時，皆有助於增加以兩種IPA為主體材料之液胞的物理穩定性，應該是因為DHDP或膽固醇皆會增加分子之間的交互作用所致。然而在HTMA-HS/DHDP混合比例為7/3的三成分系統當中，其液胞的物理穩定性略低於HTMA-DS/DHDP混合比例為7/3的系統，推測與混合單分子層的等溫線中所發現的凝縮相之相轉行為有關。

This study investigated the effects of added dihexadecyl phosphate (DHDP) and cholesterol on the monolayer behavior of ion pair amphiphiles (IPAs) with different alkyl chain lengths, hexadecyltrimethylammonium-hexadecylsulfate (HTMA-HS) and hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), by analyzing molecular interaction and mixed monolayer stability with surface pressure-area isotherms, surface potential-area isotherms, and relaxation curves. The isotherms indicated that DHDP and cholesterol possessed the condensing effect on the mixed monolayers. In addition, at a surface pressure of 30 mN/m, the stability of the mixed monolayers became higher with DHDP addition, but became lower with the cholesterol addition. Moreover, there was a phase transition in the solid phase region of the condensed monolayers in the presence of cholesterol. It was suggested that the molecules rearranged at high surface pressures with cholesterol addition. It was noted that the tilt angle of the molecular alkyl chains was increased in the condensed monolayers and was decreased in the expanded monolayers. The physical stability of catanionic vesicles fabricated from the IPAs was improved with the addition of DHDP and cholesterol, which could be attributed to the enhanced interaction between the molecules. However, when the mole ratio of IPAs/DHDP was 7/3, the physical stability was poorer in HTMA-HS-formed vesicles than in HTMA-DS-formed vesicles, which might be related to the phase transition characteristic observed for the condensed monolayers.

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