本研究目的在於探討一種蛋白質合成抑制劑（cycloheximide）對甲基安非他命所建立的制約場地偏好（conditioned place preference，簡稱CPP）之獲得與消退所造成的影響。使用甲基安非他命每公斤體重二毫克的劑量對老鼠建立CPP後，該CPP透過每天重複的測試仍可維持一段相當長久的時間，甚至可以超過五十天之久。文獻指出有二種實驗策略可以讓甲基安非他命所建立起來的CPP加以消褪，一為每天重複測試，另一為強迫式消褪訓練。我們發現，利用上述任何一種消褪策略之前經過cycloheximide前處理的老鼠，相對於生理食鹽水前處理的老鼠已經展現CPP的消褪，該群老鼠仍維持CPP，尚未消褪，此一研究結果指出，甲基安非他命建立起CPP後的消褪須牽涉到蛋白質的合成。但有趣的是，經由cycloheximide前處理的老鼠似乎無法有效地阻斷或減弱甲基安非他命造成CPP的獲得。
從上述實驗中，我們得到三個結論：（一）甲基安非他命所建立起來的CPP可以維持相當長久的一段時間，而且利用每天重複測試，將老鼠暴露在當初用藥物制約的環境中，不再給予藥物，CPP仍很難加以消褪；（二）甲基安非他命所建立CPP的消褪需要新蛋白質的合成；（三）甲基安非他命CPP的獲得是否牽涉到蛋白質的合成須進一步的研究才能確立。

This study investigated the role of a protein synthesis inhibitor, cycloheximide, in the acquisition and extinction of methamphetamine (MA)-induced conditioned place preference (CPP). I demonstrated that the maintenance of the rapidly-established MA (2 mg/kg, ip)-associated CPP could be long-lasting and over fifty days, whereas the fluctuation was evident throughout the extinction process of repeated daily retention tests. Most importantly, cycloheximide-pretreated mice maintained the established MA-induced CPP and did not exhibit extinction of MA-associated CPP as compared to the saline-pretreated mice when they underwent a similar protocol of repeated daily retention tests or a forced extinction training. These results indicate that the protein synthesis is involved in the extinction of MA-induced CPP. Interestingly, cycloheximide-pretreated mice did not seem to exhibit attenuated acquisition of MA-associated CPP. I conclude that (1) MA-induced CPP could be maintained in a long-term manner and such CPP is reluctant to extinguish even animals' repeated exposure to the previous conditioning environment at a drug-free status, (2) the extinction of MA-induced CPP requires a protein synthetic process and (3) the involvement of protein synthesis in the acquisition of MA-indeuced CPP seems to be indeterminate and requires further investigations.

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