利用化學接枝法(grafting onto)將高分子接枝在基材表面上去改變原本材料的表面性質，並保有材料本身原有性質(bulk property)，是很常見的表面改質手法及技術之一。在本篇研究中，為了改善鈦金屬基材表面的血液相容性，利用傳統的自由機共聚合將含有亞磷酸官能基的6-acryloyloxy hexyl phosphonic acid (AcrHPA) 以及末端具備氫氧根官能基之4-hydroxybutyl acrylate (HBA) 合成雙功能性共聚高分子。該共聚高分子的亞磷酸官能基不僅可以和鈦金屬基材表面形成穩定的共價鍵結，還可以藉由高分子的氫氧根官能基提升鈦金屬基材表面之親水性質以達到抑制血小板貼附並達到改善材料表面的血液相容性。
　　在研究中，使用核磁共振光譜(Nuclear magnetic resonance spectroscopy)對單體及共聚高分子進行結構及組成之鑑定，之後將該共聚高分子分別以自然滴製法(drop-coating)及旋轉塗佈法(spin-coating)覆蓋於鈦金屬基材表面上並加熱使高分子與表面產生共價鍵結反應，並依序利用靜態表面接觸角測量(Static water contact angle analysis)表面親疏水性、掃描式電子顯微鏡(Scanning electron microscope)觀察改質層厚度、電子能譜儀(X-ray photoelectron spectroscopy)分析表面元素組成。最後藉由體外血小板吸附實驗(In vitro platelets adhesion)探討經高分子改質層修飾後之鈦金屬基材表面的血液相容性。由實驗結果可以發現隨著單體HBA的進料比增加，表面的高分子改質層的親水性質也隨之上升，導致鈦金屬基材表面的血液相容性有所改善。在此篇研究中，提供了一個有效的表面改質方法並且成功地使鈦金屬基材表面達到抑制血小板貼附及活化的效果。

Grafting polymer onto the surface has being used as a process to modify the surface properties. In this study, in order to improve hemocompatibility of titanium, the surface of substrates were modified with a layer of novel multi-functional copolymer, which consists of 6-acryloyloxy hexyl phosphonic acid (6-AcrHPA) and 4-hydroxybutyl acrylate (HBA) monomers synthesized by traditional free radical copolymerization. The copolymers do not only bind to titanium surface stably with phosphonic acid function groups, but also prevent platelet adhesion effectively with the hydroxyl function groups, which made surface more hydrophilic. The prepared monomers and polymers were demonstrated by nuclear magnetic resonance spectroscopy (NMR). Then the polymers were drop-coated and spun-coated onto the electropolish-pretreated titanium and heated for covalent-bounded onto the substrate. The obtained modified layers were subsequently characterized by static water contact angle measurements (CA), scanning electron microscope (SEM) to determine the thickness of modified layer, and x-ray photoelectron spectroscopy (XPS). Furthermore, the hemocompatibility of polymer films was characterized through in vitro platelets adhesion assay. The results indicated that no matter which coating method was utilized, the increase of HBA feeding ratio can increase surface hydrophilicity and reduce the degree of platelet adhesion and activation. The surface modification in current study showed an effective strategy for developing platelet compatible titanium-based biomedical devices.

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