本研究探討代表性臭味物質-cyclocitral在代表性湖庫中之產生及分佈特性，及其氧化處理可能性。研究中分成四個部份，包括藻華水中臭味種類之鑑定、臭味物質濃度與微囊藻之相關性、金門太湖水庫中臭味物質之流佈情形、以及臭味物質之氧化特性等。
研究中首先以(Flavor profile analysis, FPA)、固相微萃取法(solid-phase microextraction, SPME)配合氣相層析質譜儀(GC/MS)，以及氣相層析嗅覺分析儀(Sensory GC)，鑑定金門太湖藻華產生的臭味類型及臭味物質，並且以顯微鏡鏡檢水體中藻相及針對微囊藻進行計數(cell counting)。研究中發現微囊藻華中產生之主要揮發性臭味物質為木頭味物質β-cyclocitral、腥味物質6-Methyl-5-Hepten-2-ol及2,4-Decadienal、藥草味物質2-cyclohexene-1-one、土味物質Geosmin、及其他腐敗酸臭味等，其中木頭味物質β-cyclocitral是微囊藻藻華水體中之最主要臭味物質，而其產生濃度與水體中微囊藻數量具有正相關性。
為了瞭解其他藻類與水體中β-cyclocitral濃度的相關性，因此在實驗室培養純化之微囊藻(Pcc7820)、球囊藻(Chlorella sp.)、顫藻(Oscillatoria sp.)、舟形藻(Narvicula sp.)，並且分析其生長期間產生之β-cyclocitral濃度變化，實驗中發現培養中的微囊藻能產生大量的β-cyclocitral，平均產量為16 fg/cell，其他藻類之培養基中並無法產生大量的β-cyclocitral。
太湖取水口多次不同深度的採樣中發現，在春天水溫較高時(20~25°C)，β-cyclocitral濃度及微囊藻數量在水面之濃度較高，在湖底的濃度較低，但是在冬天水溫低時(<20°C)，臭味物質2-MIB、Geosmin、及β-cyclocitral濃度與微囊藻數量在不同水深之間並沒有明顯的變化情形。太湖不同湖邊位置採樣結果發現，太湖之風向會影響微囊藻華出現的位置，上浮的微囊藻會隨著風向往某個方向的岸邊累積成藻華的現象，並且在微囊藻華中臭味物質β-cyclocitral濃度分別高達1,500 μg/L，Geosmin也有50 ng/L以上的濃度，而微囊藻的數量也高達6×108 cells/mL，並且也發現β-cyclocitral的濃度與水中的微囊藻數量具有正相關性，但是β-cyclocitral與水體中總微囊藻毒素並沒有明顯的正相關性。
對於β-cyclocitral氧化實驗發現，高錳酸鉀雖然對於去離子水中的β-cyclocitral去除效果良好，但是對於原水中β-cyclocitral的去除反而不佳，而氯雖然對於去離子水中的β-cyclocitral沒有明顯的氧化力，但是在原水氧化實驗中發現，氯反而可以有效地控制及去除水體中β-cyclocitral的濃度，此部分差異待後續更詳細之研究。

The distribution and treatment of a typical odorant and metabolite from Microcystis, -cyclocitral, in several reservoirs were investigated in this study. This study is consisted of four parts, the identification of the odorants in algal bloom water, the relationships between the odorant and algal cell density, the diurnal and spatial and distribution of the odorant in Tai-Lake, and the oxidation characteristics of the odorant.
Two sensory methods, flavor profile analysis (FPA) and solid-phase micro-extraction (SPME) coupled with gas chromatograph/mass spectrometry detector (GC/MSD) were employed to identify the odor types and corresponding odorants in the water of Tai-Lake, Kinmen. The major volatile odorants in the Microcystis bloom include a woody odor compound, β-cyclocitral, two fishy odor compounds, 6-methyl-5-hepten-2-ol and 2,4-decadienal, a herbal odorant compound, 2-cyclohexene-1-one, a earthy odorant compound, geosmin. Among the odorants detected, β-cyclocitral is the major odorant present in the algal bloom of Microcystis, and the concentrations well correlated with the cell concentrations of Microcystis.
To characterize the production of β-cyclocitral in different algae, purified cyanobacteria, Microcystis Aeruginosa PCC7820 and Oscillatoria sp,, a green alga, Chlorella sp., and a diatom, Narvicula sp., were incubated in the laboratory and were analyzed for a few odorants. Microcystis Aeruginosa PCC7820 was the only one that produces high concentration of β-cyclocitral at 16 fg/cell. All other algae only produce minor amount of β-cyclocitral in the experiments.
The water samples at different depths near the intake of Tai-Lake suggested that the concentrations of β-cyclocitral and Microcystis spp. changed with time and water depth. In late spring where water temperature was at 20-25°C, the concentrations of β-cyclocitral and Microcystis spp. were higher near the water surface. However, in winter time where the temperature was less than 20°C, the concentration of 2-MIB, geosmin, and β-cyclocitral, and Microcystis spp. remained almost constant at different water depths. In addition, the occurrences of algal blooms were relevant to the wind direction, mostly concentrated in the downwind shores. In the algal bloom samples, up to 1,500 μg/L of β-cyclocitral and 50 ng/L of geosmin, and 6×108 cells/mL of Microcystis spp. were detected. The concentration of β-cyclocitral well correlated to the cell density of Microcystis spp. in the samples, and however, did not correlate to the microcystin concentration in the samples.
The oxidation experiments suggested that β-cyclocitral can be easily oxidized by permanganate in de-ionized water, but were more resistant in raw water . For chlorination, β-cyclocitral was very resistant in de-ionized water, and was much easier to be oxidized in raw water. The discrepancy of the oxidation of β-cyclocitral by chlorine and permanganate in de-ionized water and raw water suggested that further study is needed to understand the reaction mechanisms.

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