木酚素富含於植物中，而其中以亞麻籽內的木酚素含量最高。木酚素具有預防癌症及心血管疾病、降低膽固醇等功效，亦可被添加於健康食品中；且在人體腸道中，木酚素會被分解成腸內酯(enterolactone) 及腸二醇(enterodiol)，兩者經證實具有良好的生理活性，因此木酚素在醫藥及食品營養方面有其重要性。現如今已發展出許多萃取木酚素的方法，但因使用有機溶劑，會造成環境的汙染且對人體有害，因此，本研究期能在安全、環保的前提下提升萃取效率與產量。
故本實驗首先使用短鏈醇萃取木酚素，期能得到木酚素的最大含量；接著，希望可以用食用級的混合非離子型界面活性劑水溶液，並和以超臨界二氧化碳取代有機溶劑萃取木酚素的結果互為比較。而木酚素中主要的成分為開環異落葉松樹脂酚二葡萄糖苷(secoisolariciresinol diglucoside , SDG)，故本實驗選擇SDG作為目標萃取物並以HPLC做定量及定性的分析。
短鏈醇萃取結果顯示，乙醇的萃取能力隨著時間增加而增加，相對地，甲醇的萃取能力與時間沒有顯著的關係；然而，甲醇輔以0.5 N氫氧化納萃取可得最大萃取量，每克亞麻籽可得14.43毫克的木酚素。界面活性劑水溶液萃取結果顯示，單一界面活性劑水溶液以Tween 20的萃取效果最佳；Span 20/PE 64混合界面活性劑水溶液的萃取能力隨著濃度的減少而下降，也隨著時間的增加而上升，並且，最大萃取量為每克的亞麻仁籽可得11.56毫克的木酚素。超臨界二氧化碳萃取結果顯示，溫度以及壓力對萃取量無顯著的影響，而當萃取溫壓為40 C和35 MPa有最大的萃取產率。而比較三種萃取方式後，以混合界面活性劑水溶液萃取廢棄亞麻籽較易應用於食品及醫藥中。

In this work, three extraction methods are mainly attempted: extraction by short-chain alcohols, extraction by nonionic surfactants, and extraction by supercritical CO2 (SFE-CO2). The HPLC was employed to analyze qualitatively and quantitatively the components of flaxseed lignans, in which the secoisolariciresinol diglucoside (SDG) is the most abundant. The methanol with alkaline hydrolysis resulted in a highest yield of lignans (14.43 mg/g flaxseed). In the case of non-ionic surfactants, the results of Span 20/PE 64 mixed non-ionic surfactants solution extraction showed that extraction capacity is lessened with decreasing concentration of surfactant and amount of lignans extracted increased with a prolonged extraction time. The maximum extraction yield of lignans by Span 20/PE 64 mixed non-ionic surfactants was 11.56 mg/g flaxseed. The results of SFE-CO2 showed that the maximum extraction yield of lignans occurred at 40 C and 35 MPa.

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