三氯乙烯與甲苯是地下水中常見的污染物。這兩類污染物皆屬易揮發性有機物，一旦洩漏至環境中通常都會造成大範圍且不均勻分布的污染，因此不利於傳統物理整治。對於這類型的污染場址，生物修復被視為可行的整治方案。考量熱力學與微生物代謝有機物特性的關係，以厭氧還原脫氯與好氧破環的整治策略來分別對三氯乙烯與甲苯污染場址進行整治被認為是最合適且有效的方案。
可惜的是，儘管含氯乙烯與苯環類化合物在整治上都已有成功的案例，但若是碰到兩類污染物同時共存在的場址，採用單一策略來進行生物修復卻被視為是困難的，無論是採用厭氧或是好氧的策略都不容易達到同時去除兩種污染物地目的，即便近期已有研究提出厭氧/好氧兩階段式等的策略作為去除複合污染物的手段，去除效率偏低與相對複雜的操作模式仍限制其未來的應用性。因此，以生物法進行複合污染場址的整治仍然是具有挑戰性的。
本研究開發了一套同時降解三氯乙烯與甲苯的新策略，並採用批次式反應器進行可行性的測試，研究藉由微量供氧的方式調整反應槽內氧化還原電位在微好氧的範圍內(ORP: -300±100 mV)變動。最終結果顯示，實驗組在DO: 0.5 mg/L的溶氧控制下成功觀察到TCE 與甲苯同時降解，相較之下，控制組 (絕對厭氧) 僅觀察到三氯乙烯的還原脫氯降解；除此之外，逆轉錄聚合酶鏈反應(Reverse transcription polymerase chain reaction, RT-qPCR)分析也顯示tceA,vcrA, catechol 2,3-dioxygenase (C23O) 等相關功能性基因在實驗組操作過程中都有存在，其中優勢代謝基因tceA與C23O表現量分別可達到8.0×103~ 6.9×106 與 4.05×104~ 5.35×105 copies/mL。這些結果為TCE還原性脫氯與甲苯的好氧降解兩大機制共存提供了直接證據。總體而言，本研究證明了同時去除污染物的可行性，所得成果能為複合型污染物廠址在現地生物修復上提供一個有科學依據的參考。

Trichloroethene (TCE) and toluene are volatile organic compounds that are often simultaneously detected in contaminated groundwater. They can be practically remediated using the bioremediation approach. When a site contains these two contaminants, a choice must be made between aerobic or anaerobic bioremediation. Although a two-stage bioremediation strategy where anaerobic and aerobic degradation processes are performed independently has been proposed, the complete bioremediation of such a site is challenging. In this study, a strategy for stimulating the simultaneous degradation of trichloroethylene and toluene under microaerobic conditions is developed and tested using sequencing batch reactors. The results show that the reactor under microaerobic conditions with dissolved oxygen content of ~0.5 mg/L achieved the complete reductive dechlorination of trichloroethylene to ethylene along with aerobic toluene degradation. Catechol 2,3-dioxygenase (C23O), tceA, and vcrA were detected during operation using quantitative reverse transcription polymerase chain reaction. Furthermore, tceA and C23O were the two dominant genes, with concentrations of 8.0×103 - 6.9×106 and 4.05×104 - 5.35×105 copies/mL, respectively. These results provide direct evidence of the simultaneous reductive dechlorination of TCE and aerobic degradation of toluene. The results demonstrate the feasibility of the simultaneous removal of pollutants and can provide a reference for the development of a complete bioremediation method for sites with multiple contaminants in situ.

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