幹細胞可分化成多種細胞，其中亦包含血管內皮細胞，然而，諸多因素導致幹細胞取得不易且分化效果不彰。本實驗室先前研究發現，可利用流體剪力模擬血流，結合化學因子，刺激幹細胞分化形成內皮細胞，然其分子調控機制尚不明。多項研究指出在流體剪力刺激下，會活化幹細胞的生長分化，調控動靜脈內皮細胞分化。
本研究將以人類臍靜脈內皮細胞(human umbilical vein endothelial call, HUVEC)建立腦缺氧動物模型，以利流體剪力刺激分化後之細胞應用。在腦缺氧動物模型中，給予臍靜脈內皮細胞，將降低腦損傷，並減少大腦細胞死亡。層流(laminar)刺激後之臍靜脈內皮細胞較未刺激之細胞無明顯修復效果。紅黴素(erythromycin, EM)處理後之臍靜脈內皮細胞具有較高CXCR4表現。在不同層流及紅黴素結合方式刺激下，層流刺激後再以紅黴素處理之細胞具有較多CXCR4表現，並有高度修復大腦之效果。在此動物模型中，我們成功建立內皮細胞修復大腦組織模型；並可藉由增加CXCR4表現，而增加內皮細胞修復效用。

Stem cells can differentiate to endothelial lineage as a source for tissue engineering of vessels. However, the rarity of cell source and low differentiation efficiency are the main limitation for stem cell therapy in vessel regeneration. Our previously study demonstrated a successful endothelial cell (EC) differentiation by integration of biomechanical force and chemical factors, but the mechanism is still unknown. Furthermore, we build up a cell therapy of brain hypoxic-ischemia (HI) model by using human umbilical vein endothelial cells (HUVECs). The differentiated cells will apply to this animal model in the future. By static HUVECs transplantation in brain HI model, brain injury and cell death were decreased. Laminar shear stress (LSS)-treated HUVECs group in animal model had not significantly repair function than static HUVECs. Previously research indicates that CXCR4, a chemoattractant receptor, is reduced by LSS. One paper showed that erythromycin (EM) increased CXCR4 expression. Therefore, we combined LSS with EM to stimulate HUVECs. We found that EM treatment after LSS stimulation of HUVEC transplantation was useful for rescuing brain injury. In this study, we built up successful cell therapy for brain injury model. Furthermore, repair function of ECs can be enlarged by increasing EM-induced CXCR4 expression.

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