現今牙科複合樹脂填補是藉由與牙本質膠原蛋白形成混合層產生鍵結，但內生性膠原蛋白酶會降解膠原蛋白、影響其生物穩定性，進而降低牙本質黏著的耐久性。已有研究證實多酚類化合物可穩定牙本質膠原蛋白基質並增加樹脂與牙本質粘合介面的機械性質。然而多酚類化合物結構的複雜性和天然植物提取物的批次間差異仍可能妨礙我們對其穩定作用機制的深入理解，也可能對進一步應用開發構成阻礙。因此，本研究目標研究多巴胺（具兒茶酚官能基之生物分子）促進牙本質膠原蛋白穩定性的潛力。
實驗中，完全脫鈣的牙本質樣本以0.1至0.001 M的多巴胺處理10分鐘，而表面脫鈣的牙本質樣本則處理5分鐘以更接近臨床情況。樣本隨後以膠原蛋白酶降解，並用FTIR光譜，橫截面SEM影像和降解後乾重減少量研究多巴胺處理對生物穩定性的影響。另外亦採用光學同調斷層掃描技術對真空乾燥後全脫鈣牙本質片的二維收縮進行定量，並以FTIR光譜研究多巴胺在牙本質表面的吸附行為。
結果部分，FTIR差異光譜證實多巴胺在牙本質上的緊密吸附，並顯現出多巴胺和牙本質基質之間的交互作用。在膠原蛋白酶降解後，10分鐘所有測試濃度之多巴胺處理組之降解量皆顯著減少。於表面脫鈣牙本質樣本處理5分鐘各組中，自FTIR光譜和SEM橫截面影像則會發現多巴胺在0.001 M濃度下最為有效。另外，全脫鈣牙本質樣本的二維收縮亦於0.001 M多巴胺處理組中有顯著降低。
本實驗證明多巴胺在低濃度、臨床可接受的處理時間內即可顯著提升牙本質膠原蛋白抗降解能力，並讓我們對酚類化合物及牙本質膠原蛋白之間的相互作用機制有更多理解。研究中發現多巴胺濃度、牙本質尺度、處理時間和穩定作用之間的交互關係將為相關研究開闢新的方向。

Contemporary dental composite restoration relies on the infiltration and hybridization with dentin collagen to establish the bonding to dentin. Due to the presence of endogenous collagenolytic enzymes, the inherent instability of dentin collagen poses a major threat to the durability of the bonding interface. Polyphenolic compounds have been proved to stabilize dentin collagen matrix and increase mechanical properties of resin-dentin bonding interface. However, the complexity of the structure of polyphenolic compounds and lot-to-lot variations of naturally-derived plant extracts remain an obstacle to a deeper understanding of the stabilization mechanism and development of further applications. Therefore, this study was aimed to investigate the dentin stabilization potential of dopamine, a phenolic biomolecule with catechol functional group.
In the experiment, fully-demineralized dentin specimens were treated with 0.1 to 0.001 M dopamine for 10 minutes, while surface-demineralized specimens were treated for 5 minutes to better simulate the clinical situation. Subsequently, the biostability of dopamine-treated demineralized dentin specimens was investigated with FTIR spectroscopy, cross-sectional SEM images and degradative weight loss after challenged by collagenase; moreover, the two-dimensional shrinkage behavior of fully-demineralized dentin slab after vacuum-dried was characterized with optical coherence tomography, and the adsorption of dopamine and its interaction with dentin collagen matrix was investigated with FTIR spectroscopy.
FTIR difference spectra demonstrated the interaction and tight adsorption of dopamine onto dentin collagen matrix. After challenged by collagenase, dopamine of all concentrations significantly reduced degradative weight loss by treatments for 10 minutes, while FTIR spectroscopy and cross-sectional SEM images suggested that 0.001 M dopamine treatment on surface-demineralized dentin specimens was most effective to maintain collagen structure. Similarly, the two-dimensional shrinkage significantly reduced only in 0.001 M dopamine treated group.
The results demonstrated the anti-proteolytic effect of dopamine treatment on dentin collagen in clinically-relevant treatment time at a low concentration. This study provided mechanistic insights into the interaction between phenolic compounds and dentin collagen in regards of the interplay among concentration, scale, treatment time. The protective effect of dopamine treatment found in this study will open up new directions for the future studies.

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