近年反聖嬰的現象造成氣候變化劇烈，使作物的產量下降。先前實驗室已發現SlWRKY1基因在冷、鹽和青枯病 (Ralstonia solanacearum) 逆境下其表現會增加。本研究觀察到SlWRKY1在正常情況下有些微表現，特別的是SlWRKY1在蕃茄果實轉色時期 (pink stage) 的表現量最高。並且SlWRKY1也會受到鹽與chilling (0 ℃) 處理而提高表現量。SlWRKY1的胺基酸序列中含有兩個WRKY轉錄因子的保守性 (conserved) 功能區 (domain)。從演化樹分析發現SlWRKY1和屬於第一類和病害反應相關受冷誘導的AtWRKY33及 OsWRKY53最為相似，因此SlWRKY1屬於第一類的蕃茄WRKY轉錄因子。藉由細胞定位分析 (cellular localization) 發現SlWRKY1主要表現於細胞核。本研究也將35S:SlWRKY1及SlWRKY1專一性RNAi載體轉殖到蕃茄中。將野生株、SlWRKY1ox和SlWRKY RNAi 基轉蕃茄以0℃處理後發現，相對於野生株SlWRKY1 RNAi的轉殖株對低溫較具耐受性。相對於野生株SlWRKY1ox 的轉殖株則對冷較敏感。SlWRKY1可能參與番茄冷逆境下的反應。

Recent years the counter-Saint infant's phenomenon caused the climatic change to be fierce, causes the crops the decline of production. In previous study, our lab found SlWRKY1 transcription factor, was induced by salt-, chilling-stress, and Ralstonia Solanacearum infection. SlWRKY1 was also highly activated at pink fruit stage. SlWRKY1 contained two WRKY conserve 60 amino acid regions. The result of phylogenetic tree analysis clearly indicated that SlWRKY1 was similar to Os05g27730 and AtWRKY33 which belong to group I WRKY protein. Os05g27730, named as OsWRKY53, is also a chilling-induced rice WRKY protein. Transient expression of SlWRKY1-GFP fusion protein showed that SlWRKY1 located at nucleus. We already obtained several SlWRKY1ox and RNAi tomato plants. These transgenic plants were confirmed by Southern blot analyses. The foreign SlWRKY1 transcripts were highly expressed in SlWRKY1ox plants. Expression of endogenous SlWRKY1 was repressed in SlWRKY1 RNAi plants after chilling treatment. Transgenic SlWRKY1ox plants were more sensitive to chilling-stress. Contrastively, the RNAi plants were more tolerance to chilling-stress. SlWRKY1 may involved in tomato chilling response.

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