摘要
　　膠原蛋白是一種具有良好生物相容性之可降解材料，廣泛應用於組織工程領域上，尤其是在傷口敷料及人工皮膚方面。以膠原蛋白作為支架建構人工皮膚，是皮膚組織工程研究的重要方向。在製備多孔性膠原蛋白支架的過程中，膠原蛋白支架的孔徑、孔隙率、支架厚度等因素都會受製程的影響。本研究目的有二：其一為藉由高解析度電腦斷層掃描（micro computed tomography：Micro CT)建立一簡單且可靠的方法來評估膠原蛋白支架的孔洞結構，進而評估製程；其二，由於Micro CT目前在軟組織應用方面不多，所以本研究希望找出適合掃瞄膠原蛋白等軟組織之Micro CT的參數，以拓展micro CT將來的應用。因此本研究藉由Micro CT切片之優點，如不需處理試片、取得影像速度較快、不會損傷試片、可得連續切片等，配合自行撰寫的MatLab程式計算得其孔隙度及連接性，並佐以Micro CT原廠Skyscan所提供之應用軟體建立3D model，得到3D孔徑分佈等資訊。本實驗共探討四種製程之孔隙度及孔徑分佈，各製程的孔隙度值與孔徑分佈有明顯不同，將來可由此推測製程之變異；且由程式發現本研究所評估的膠原蛋白支架之連通性非常高。結果顯示Micro CT切片較傳統切片，可將膠原蛋白支架的孔洞結構做更加完整的量化評估，使未來相關材料的製程品管多一快速方便之方法。

Abstract
　　Collagen is a biomaterial with great biocompatibility and biodegradable which is widely used in the field of tissue engineering as the scaffold, especially for wound dressing and artificial skin. The pore size, porosity, thickness and other structure factors of scaffold would be influenced by the fabrication process. There are two objectives of the study：1. To evaluate the structure of collagen scaffold with micro computed tomography (Micro CT) approach. 2. In order to extend the application of Micro CT, establishing scanning the parameters for soft tissue with Micro CT evaluation. Micro CT sectioning have at least the follow advantages：less human effort, less time consuming, without damaging the specimen, and continuous data. The study used self-developed MatLab programs to evaluate the porosity and connectivity of the scaffold structure, along with the software provided by the Micro CT to get the 3D pore size distribution. Among the four kinds of fabrication process, each scaffold had different porosity and pore size distribution, which indicated the importance of collagen scaffold evaluation in justification the fabrication process. The results showed that 3D reconstructed from Micro CT images could be useful in the quantitative evaluation of scaffold which could further be used at the process justification.

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