近年來，實驗室自動化一直是一個新興的議題。許多製藥和生命科學實驗室都熱衷於高通量自動化，以節省重複和繁瑣的實驗室工作的時間。尤其是液體處理，需要自動化，因為它是實驗室的標準和必不可少的程序。許多研究已經證明了為實驗室目的修改3D打印機的可行性，特別是用於液體處理。然而，這些研究缺乏涉及液體抽吸、分配、輸送、移液和混合的徹底的小體積液體處理。一些改造後的 3D打印機甚至需要注射泵、和霧化器等外部設備來執行液體處理。
我們的研究主旨在於利用商用金屬零件、一次性注射針筒等容易獲得的零組件與設計的3D列印部件，對商用3D列印機進行改裝並對其內部程式進行自定義編寫後將raspberry Pi與之相結合成可利用電腦輸入G-code進行控制之可放置於培養箱內的小型自動化設備平台。
在我們的研究中,透過一系列驗證我們開發系統的液體分配精確度以及移動確認其滿足使用上的精確度,並利用設計的接頭使的移動精度上獲得進一步的提升,額後將開發的系統用於藥物篩檢實驗,驗證了此系統在未來應用於實驗室自動化系統的可行性。
使其能夠達成生醫實驗室的特定目的及需求並在簡化繁瑣重複性動作和製備過程之餘，能使更大程度減少因要照看細胞而付出的時間成本，不僅能使研究人員更能專心致力於研究工作當中更能大大緩解實驗室對細胞維運的人力開支。
關鍵字 : 3D列印機、3D列印、液體分注

In recent years, laboratory automation has emerged as a prominent topic. Many pharmaceutical and life science laboratories are embracing high-throughput automation to save time on repetitive and tedious laboratory tasks. Liquid handling, in particular, demands automation as it is a standard and essential procedure in laboratories. Several studies have demonstrated the feasibility of modifying 3D printers for laboratory purposes, especially for liquid handling. However, these studies lack comprehensive coverage of small-volume liquid handling, including liquid aspiration, dispensing, transport, pipetting, and mixing. Some modified 3D printers even require external devices such as syringe pumps and atomizers to perform liquid handling tasks.
Our research aims to combine commercially available metal parts with custom-designed 3D printed components, integrated with Raspberry Pi for computer control and customizable programming. By retrofitting and assembling these components, we create a small-scale automated device platform that meets specific needs and requirements of biomedical laboratories.
In our research, we verified the precision of liquid distribution and the accuracy of movements in our developed system to ensure it meets the required standards. We further enhanced the movement precision by designing specific connectors. Subsequently, we applied the developed system to drug screening experiments, confirming its feasibility for future use in laboratory automation systems.
This platform can be placed inside an incubator and simplifies repetitive actions and preparation processes. It significantly reduces the time and effort required for cell maintenance, allowing researchers to focus more on their research work and alleviating the labor-intensive tasks associated with cell culture in the laboratory.
Key words : 3D printer、3D printing、Liquid handling

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