藻藍素是一水溶性的色素蛋白複合體，可從螺旋藻中萃取得到。藻藍素具有天然的顏色，現今被應用在健康飲料、食品著色劑、以及化妝品領域。近年來藻藍素的藥理性質像是抗氧化、抗腫瘤等活性已被逐步證實。
本研究中發展出一套可取代層析管柱的簡易分離純化方式，藉此得到高純度的藻藍素。程序上先利用酵素水解方式溶解螺旋藻細胞壁，再配合磷酸鹽緩衝溶劑萃取以得到高起始純度的藻藍素。後續的純化製程則使用飽和硫酸銨沉澱目標色素，最後利用切線流過濾法搭載截留分子量薄膜以去除小分子雜蛋白，並同時達到去除鹽類以及產品濃縮的效果。
由於藻藍素對於溫度、光、微生物等因子相當敏感，其本身的顏色容易受到這些因子影響而褪色，並失去其生理活性，限制了其在商業上的應用。因此，本研究試圖利用不同方式在室溫下維持藻藍素的穩定性，方法包括將藻藍素冷凍乾燥成粉末狀保存，以及在藻藍素溶液中添加適當的保存劑。結果指出，當EDTA-2Na添加劑量為藻藍素含量的1/5時，pH6.0的藻藍素溶液在室溫下有良好的保存效果，其抗氧化活性在室溫下經過35天後可維持在起始值的90%以上。

C-phycocyanin (C-PC) is water-soluble and can be easily extracted from Spirulina sp. as a protein-pigment complex. C-PC has been used in health drinks, food colorant and cosmetics. Furthermore, some of the medicinal properties such as antioxidant, anticancer activity are proven recently.
Here we developed a simple method for obtaining C-PC with high purity without the use of ion exchange chromatography. The procedure is based on enzymatic hydrolysis to dissolve outer cell wall of Spirulina sp. biomass, followed by solvent extraction to gain crude extract of C-PC with high initial purity, then purification by pigment precipitation through ammonium sulfate saturation, finally tangential flow filtration was implement to desalt and concentrate product.
The commercial application of C-phycocyanin is limited due to its sensitivity to heat, light, microorganism, etc, which results in precipitation and fading of the blue color, and C-PC will lose its biological activity. In this study, we have tried to use different methods to stabilize C-PC under room temperature, which included making C-PC into powder form and adding preservatives into C-PC solution. Finally, low dose of EDTA-2Na to C-PC (weight ratio 1:5) was found to be an effective preservative in pH6.0 C-PC solution, which can maintain its 90% initial activity after 35days under room temperature.

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