近年來微機電系統（Micro Electro Mechanical System, MEMS）技術迅速發展，並引發了微全分析系統（Micro Total Analysis System, μ-TAS）的研究，此系統具有體積小、檢測只需少量樣本、反應時間快等優點，再結合微製程技術便可達到減少成本的效果，且目前已有細胞研究、細胞分離、藥物檢測等多方面應用。
本研究目的在於分離多尺度之微粒，藉由數值計算建構本研究管道的模擬系統，並利用數值模擬結果與流阻理論作管道最佳化設計與評估。
實驗部份，本研究利用微流體力聚焦法與非對稱流阻設計為粒子分離主要機制，並且探討擠壓段中的流量分布以更精確預估微粒出口位置。微管道製作方式是以塗佈感光性光阻，以微影蝕刻的方式在矽晶圓轉移圖案，再利用polydimethylsiloxane（PDMS）翻模圖形而後接合於玻璃片上，成為本研究的微流體晶片系統。
由實驗結果與數值模擬成功交互比對驗證，本研究的微流體晶片可以使2 μm、6 μm及10 μm粒徑的微粒有不同的位移路徑，達成分離之目的。

Micro-Electro-Mechanical-System（MEMS）is developed rapidly in recent years, and it facilitates the study of micro total analysis system（μ-TAS）. The system offers several potential advantages. It needs very small volume of samples and reagents, produces little waste, and offers short reaction and analysis time. It can decrease the cost by combing with micro-fabrication technology and has various applications for cell study、cell separation and drug detection.
In this study, we use numerical analysis to construct microchannel simulation system for multi-particle separation. The channel is designed and assessed according to flow resistance theory.
This research utilizes hydrodynamic focusing and asymmetrical exit channel resistance design to enhance particle separation. We can predict particle outlet more accurately by controling the flow distribution in pinch segment. In this study, fabrication is based on PDMS elastomer. The master is formed on silicon wafer using an epoxy-based photoresist. PDMS is cast against the master to produce molded layer containing channels which is then bonded on a glass to form the chip.
The result of the experiments and numerical simulation are compared and discussed. The particles of 2 μm、6 μm and 10 μm in diameter can be separated in our microchannel design through their different displacement paths.

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