全球氣候變遷嚴重影響了動植物的生長分布和農業的生產活動，也讓人類生存的環境更極速的惡化。台灣位於亞熱帶區域，氣象變化大且災害發生率高，每年都會面臨各種自然災害所造成的鉅大的農損，而坡地農業因為地形的關係，總是首當其沖，造成許多可怕的災難，於是如何因應全球氣候變遷，在危脆的坡地從事農業生產，或是拯救崩塌或土石流的坡地，坡地土壤的穩定度與植被的物種分布都是關鍵，過往的研究顯示，土壤微生物與植物的生長分布關係甚大，但土壤微生物與坡地土壤穩定度的關聯性則需進一步分析探討，所以本篇研究將著重在分析不同場域中土壤微生物的物種及多樣性的差異，和土壤微生物與坡地土壤穩定度的關聯性，而自然農法與慣行農法對土壤微生物多樣性的影響，則是本研究的核心。

本研究以台南市東山區作為研究場域，採集了森林、微生物農法田地和崩塌地的土壤作為研究樣本，再以次世代定序和DNA分子條碼比對的大數據分析方式，藉此了解3種場域微生物群的基因體資訊。結果顯示崩塌地的菌相與森林和微生物農法田地差異最大，且擁有2種獨特的OTU，而微生物農法田地菌相雖然與森林較為接近，但2者在特定菌門的占比還是有一定的差異。此外，雖然各採集樣點的OTU數量有些許差異，但各場域的微生物多樣性和物種數量無顯著性差異。由此顯示多樣性並非造成崩塌的主因之一，而關鍵菌種的變化可能是造成坡地崩塌的可能原因之一。

Global climate change is rapidly changing the living environment of human beings, and also affects the growth and distribution of animals and plants as well as agricultural production activities. Taiwan is a mountainous island located in a subtropical region. Every year, Taiwan faces numerous serious natural disasters and agricultural losses caused by climate imbalance. The soil microbial balance in Taiwan’s ecosystems has also been impacted by climate change, a phenomenon that is more obvious on hillsides than on flat land.

In this study, we collected soil from a forest, natural farm, and landslide area in Dongshan. Through the use of metagenomics methods, we found that the microbiota in the landslide area were different from those in the forest and natural farm. In addition, the microbiota in the forest and natural farm differed at the phylum level. Biodiversity in the soil was not significantly different between the three areas, indicating that this was unlikely to have been a cause for the landslide. By contrast, the change in microbiota might be the reason for the landslide.

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