阿茲海默氏症主要的病理特徵是神經細胞內纖維化糾結以及腦
細胞外老年斑塊，根據先前文獻研究指出老年斑塊的形成主要是由於
貝它糊蛋白的異常聚集及沉積。近期文獻更指出貝它糊蛋白可誘發神
經纖維化糾結的形成，但是影響腦中貝它糊蛋白聚集沉積的機制至今
尚未清楚。本實驗的主要目的是尋找具有腦區性的貝它糊蛋白結蛋
白，乃利用貝它糊蛋白區域性沉積之特性，探討在不同腦區域中貝它
糊蛋白之結合蛋白是否具有差異性，而此結合蛋白是否會影響貝它糊
蛋白之聚集沉積。實驗先以免疫沉澱貝它糊蛋白的方式尋找阿茲海默
氏症患者頂葉灰質、白質及小腦中有濃度差異之貝它糊蛋白結合蛋
白。實驗顯示在分子量約14~16KD 處有蛋白質因為加入外生性貝它糊
蛋白而明顯增加，因此利用質譜(LC/MS/MS)分析。從質譜鑑定結果
推測貝它糊蛋白之結合蛋白可能為血紅素貝它鏈、阿法synuclein 及
血紅素阿法鏈三個候選蛋白質。由於三個候選蛋白中有兩個屬於血紅
素，故以西方點墨法分析阿茲海默氏症及非阿茲海默症個案腦中血紅
素區域性之含量。實驗結果發現阿茲海默症頂葉灰質血紅素之含量多
於白質，更高於小腦中的含量，這與阿茲海默氏症病人腦中貝它糊蛋
的沉積的情形雷同。但是在非阿茲海默氏症個案的腦中血紅素卻無區
域性之差異。另外在時間及濃度檢測的實驗中發現在血紅素有干擾貝
它糊蛋白聚集的效果，且血紅素濃度越高阻絕貝它糊蛋白聚集之效力
越好。最後，從免疫組織染色的結果顯示血紅素與阿茲海默氏症之類
澱斑塊具有共存現象，特別是貝它糊蛋白聚集成核之處。綜合以上結
果，我們推測血紅素為貝它糊蛋白關連蛋白，且在貝它糊蛋白聚集沉
積的過程中血紅素的表現量可能被增強，由於血紅素與貝它糊蛋白的
結合阻止貝它糊蛋白凝聚。

The major pathological hallmarks of Alzheimer’s disease (AD)
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objective of this study is to search for region-specific proteins that
participate in Ab aggregation. AD parietal lobe gray and white
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proteins, alpha synuclein, hemoglobin alpha and beta chains.
Because two of three candidates were the components of
hemoglobin (Hb), the Hb concentration in different brain regions and
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pahtalogies. Furthermore, Hb was shown to prevent Ab aggregation
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