在本研究中，利用幾丁聚醣(chitosan)與三聚磷酸鈉(sodium tripolyphosphate)所形成的膠體顆粒包埋具有降解酚能力的惡臭假單胞菌(Pseudomonas putida CCRC14349)，探討包埋菌體顆粒的特性及降解酚的效能。經過最佳處理程序所製備的包埋菌體之顆粒，以批次震盪培養的方式與懸浮菌體比較降解酚的效能，結果顯示包埋菌體具有多項優勢，包括酚降解過程中保持溶液澄清、提升環境負荷的忍受度(如高pH值、低離子強度或高濃度酚的環境)以及長期的重複使用性等。而顆粒經過酚馴養後，表現出耐酸性及高離子強度的穩定結構，並藉由酚在顆粒中有效擴散係數的計算可說明，隨著重複進行酚降解馴養後，支配酚降解速率的要素將由擴散限制轉趨於反應限制。對於連續式酚降解測試，將顆粒填充於管柱中運作，利用加入過氧化氫可有效的提供菌體代謝酚所需的溶氧，並隨著停留時間的增加，酚的去除可接近於完全。

This study was aimed to investigate the efficiency of phenol degradation by Pseudomonas putida CCRC14349 that was entrapped within the gel beads prepared by chitosan crosslinked with sodium tripolyphosphate. In addition, various characteristics of these beads were evaluated as well. The efficiency of phenol degradation for the entrapped cells was optimized by changing various processing parameters, such as the amount of cells used, beads washing medium as well as acclimatization strategy. Based on the batch phenol degradation results, these entrapped cells were found to be superior to the free cells in terms of keeping the solution clear, improving the toleration to the environmental loadings (e.g., high pH, low ionic strength, high phenol concentration), and repeating usage for an extended period. After phenol acclimatization, the structure of cell-entrapped beads showed to be stable under acidic and high ionic strength conditions. Diffusion coefficient calculation has indicated that phenol degradation by the entrapped cells varied from the intraparticle diffusion control to reaction control as the beads were used repeatedly. With the increase of hydraulic retention time and the addition of hydrogen peroxide to increase the dissolved oxygen level for cell metabolism, phenol in the influent was almost completely degraded in the packed-column test.

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