生物體暴露在環境壓力下常會產生反應性氧化性物質，這些氧化性物質會造成細胞膜的傷害，形成脂質過氧化作用，進而造成氧化性傷害。芝麻油為一天然的植物油，具有抗氧化能力，口服芝麻油能清除體內多餘的自由基，而降低氧化性傷害。然而，芝麻油是否能透過皮膚吸收途徑來降低體內氧化性傷害，仍無科學上的研究證據。故本研究的目的為以大鼠模式探討芝麻油經由皮膚浸泡吸收方式，來減緩體內氧化性傷害。

本實驗給予大鼠經由腹腔注射投予不同劑量的脂多醣體，來誘發其體內氧化性傷害。投藥前，大鼠腹部的毛剃除，老鼠麻醉後腹部浸泡於芝麻油或礦物油中，然後在第0、1、2及4小時犧牲，收集其血液樣本。利用超微量化學螢光儀測量全血中的陰氧離子及氫氧離子含量，還有超氧化物岐化酵素、過氧化氫酶及麩氨基硫含量，並量測血清中的尿酸及一氧化氮，來探討芝麻油對於減緩氧化性傷害之保護機制。此外，利用膠布剝離實驗來評估以物理性方式去角質層能否增加芝麻油經皮膚吸收的能力，更快速地來減緩體內氧化性傷害。

實驗結果如下：皮膚吸收芝麻油後與脂多醣體處理比較，(1)第2及第4小時，脂質過氧化程度有顯著的被降低；(2)自由基方面，第2及第4小時，全血中的超陰氧離子能顯著被抑制，而氫氧離子只有在第4小時有顯著被抑制；(3)超氧化物岐化酵素、過氧化氫酶及麩氨基硫等抗氧化性酵素皆無差異；(4)血清中的尿酸在第2及第4小時有明顯差異，(5)而血清中的一氧化氮只在第4小時有顯著差異；(6)在膠布剝離實驗方面，經過膠布撕裂與未處理其血清中的脂質過氧化程度及一氧化氮含量無差異。

本研究的結論如下：(I)芝麻油能透過皮膚吸收，藉由抑制黃嘌呤氧化酵素活性及一氧化氮來降低超陰氧離子基及氫氧離子基的產生，進而減緩自由基所造成的體內脂質過氧化程度。(II)以物理性方式去除皮膚的角質層未能提升芝麻油對抗體內氧化性傷害的功效。

Reactive oxygen species (ROS), are generated in response to environmental stress, damage cell membrane to cause lipid peroxidation (LPO). LPO is considered as an indicator of oxidative stress. Sesame oil (SO), a type of nature plant oils, possesses antioxidant effect to scavenge oxygen free radicals. The aims of our study are (1) to examine whether soaking of SO could attenuate oxidative stress and to investigate the possible protective mechanism of soaking SO to reduce oxidative stress; (2) to demonstrate whether removing the horny layers by tape stripping could enhance percutaneous absorption of SO to reduce oxidative stress.

Abdomens of the rats were shaved with a safety razor before the experiment. LPS (0, 5, 25, 50 mg/kg, i.p.), a tool used to induce oxidative stress, was administrated to rats in our study. Under anesthesia, rats were laid on the specific container, containing the SO. Rat blood samples were collected after 0, 1, 2 and 4 h. Serum LPO levels were determined by a commercial kit. In addition, the superoxide anion (O2._), hydroxyl radicals (.OH) were also measured by the chemiluminescence analyzer and antioxidant enzymes, superoxide dismutase, catalase and glutathione were also measured. The serum uric acid and nitric oxide (NO) were also determined. On the other hand, serum LPO and NO were also measured after tape stripping.

Results indicated that (1) SO significantly suppressed serum LPO levels at 2 and 4 h, (2) O2._ and .OH radicals were also significantly reduced by SO, (3) no changes were indicated for antioxidant enzymes, (4) serum uric acid were reduced at 2 and 4 h but the serum NO only was altered at 4 h, (5) serum LPO and NO were not altered after tape stripping.

In summary, it is suggested that (I) soaking of SO may attenuate oxidative stress by suppressing xanthine oxidase activities and NO productions to decrease the generations of O2._ and .OH radicals. (II) tape stripping of the stratum cornem did not enhance the effect of soaking use of SO against oxidative stress.

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