本論文為研發一掌上型微循環(microcirculation)顯微鏡，包括光學設計與製作及影像處理部份，其功能主要為可動態地追蹤擷取微血管影像。傳統的微循環取像機構，主要是利用現有的顯微鏡改裝，因而有攜帶不便及不易於臨床檢測的缺點。因此，在這掌上型的微循環顯微鏡的開發上，於光學之設計與製作方面：1)考量血紅素的吸收與影像對比問題，利用高亮度的綠光LED來打光，以提升影像對比，並可縮小打光系統的體積；2)設計製作可攜式、低像差之透鏡顯微透鏡組來清晰放大影像，達到顯微成像目的。
於後續的數位影像處理方面：首先考量操作者的手部與患者的受測部位之左右晃動，造成影像觀察及參數計算上產生誤差，可利用影像間之二維交互相關(cross-correlation)關係來將預計觀察部位鎖定，以減少影像晃動的情形；另外，發展出可計算微循環中血液流速、血管尺寸、微血管密度與分佈比例等參數之影像處理技術。
最後，針對操作者的手部與患者的受測部位之上下晃動問題，製作ㄧ桌上型自動顯微對焦系統，利用爬山搜尋法(hill-climbing search)尋找索貝爾(Sobel)峰值的縱向成像位置，即可找尋至最清晰的影像。以此自動顯微對焦機制，將可提供日後掌上型微循環顯微鏡於克服上下晃動問題的參考依據。

In this thesis, a hand-held microcirculation microscope based on optical
design and image processing has been developed and is used to dynamically
grab capillary images. Currently, conventional microcirculation systems
based on standard microscopes have some defects such as inconvenient carry
and difficult clinical operation. Therefore, in this developed microcirculation
microscope, two new aspects are: 1) to utilize the high-brightness green LEDs
with a compact modular design to light capillarys due to heme absorption,
hence the contrast of the grabbed capillary images can be enhanced; 2) to
develop the hand-held microcirculation microscope based on low-aberration
lens design to achieve enlarged and clear imaging
To process the grabbed images based on digital image processing
technique, two main functions have been developed. One is to overcome the
transversal shaking between the operator’s hand and the patient’s measured
component based on a two-dimensional cross-correlation method and
reconstruct the capillary images with reduced shaking. The other is to develop
the physiological function to calculate the parameters of flow rate, vessel size,
blood distribution.
Finally, an autofocusing controller has been developed and is utilized to
overcome the longitudinal shaking. The clear images are locked by using a
hill-climbing method which seeks the Sobel peak value of the grabbing
images. The mechanism of the autofocusing controller can be referred to
overcome the restriction of longitudinal shaking in the further developing.

1. 易政男，微循環顯微影像之擷取與分析，中央大學光電科學研究所碩
士論文，2001。
2. 曾戎威，“微循環與健康”，基礎雜誌25，48-49 (2007)。
3. 修瑞娟，“微循環世界微循環研究的回顧與展望”，中國微循環3，5-8
(1999)。
4. N. Wertheimer and M. Wertheimer, “Capillary structure: Its relation to
psychiatric diagnosis and morphology,” J. Nerv. Ment. Dis. 121, 14-27
(1955).
5. W. C. Gibson, H. J. Bosley, and R. S. Griffiths, “Photomicrographic
studies of the nailbed capillary networks in human control subjects,” J.
Nerv. Ment. Dis. 219, 219-231 (1956).
6. A. S. Norris and J. R. Chowning, “Capillary morphology of the nailfold
in the mentally ill,” J. Neuropsychology 5, 225-234 (1963).
7. L. R. Rouen, E. N. Terry, B. H. Doft, R. H. Clauss, and W. Redisch,
“Classification and measurement of surface microvessels in man,”
Microvasc. Res. 4, 285-292 (1972).
8. Y. Kabasakal, D. Elvins, E. F. J. Ring, and N. J. McHugh, “Quantitative
nailfold capillaroscopy findings in a population with connective tissue
disease and in normal healthy controls,” Ann. Rheum. Dis. 55, 507–512
(1996).
9. B. F. Jones, M. Oral, C. W. Morris, and E. F. J. Ring, “A proposed
taxonomy for nailfold capillaries based on their morphology,” IEEE
Transactions on Medical Imaging 20, 333-341 (2001).
10. 劉育英，微循環圖譜，人民軍醫出版社，2005。
11. H. Wayland and P. C. Johnson, “Erythrocyte velocity measurement in
microvessels by a two slit method ,” J. Appl. Physiol. 22, 333-337 (1967).
12. M. Intaglietta, M. Silverman, N. R. Silverman, and W. R. Tompkins,
“Capillary flow velocity measurements in vivo and in situ by television
methods ,” Microvasc. Res. 10, 165-179 (1975).
13. 田牛，微循環，科學出版社，1980。
14. T. Eiju, K. Matsuda, J. Ohtsubo, K. Honma, and K. Shimizu, “Frequency
sihfting of LDV for blood velocity measurement by a moving wedged
glass ,” Applied Optics 15, 3833-3837 (1981).
15. J. Umetani, C. Yuzawa, E. Okada, E. Sekizuka, C. Oshio, and H.
Minamitani, “Microvascular red blood cell velocity measurement using
the image gradient method,” IEEE Engineering in Medicine and Biology
Society 2, 624-625 (1989).
16. Y. Aizu, H. Ambar, T. Yamamoto, and T. Asakura, “Measurements of flow
velocity in a microscopic region using dynamic laser speckles based on
the photon correlation,” Optics Communications 72, 269-273 (1989).
17. K. Tsukada, H. Minamitani, E. Sekizuka, and C. Oshio, “Blood flow
measurement system for microcirculation by image correlation method,”
IEEE Engineering in Medicine and Biology Society 2, 684-685 (1996).
18. D. A. Christopher, P. N. Burns, J. Armstrong, and F. S. Foster, “High
frequency continuous-wave Doppler ultrasound system for detection of
blood flow in the microcirculation,” Proceedings of the IEEE Ultrasonics
Symposium 2, 1493-1496 (1995).
19. G. Dong, E. Damiano, M. L. Smith, S. T. Acton, and K. Ley, “Detection
of microspheres in venules for venules for automated particle image
Velocimetry,” IEEE Computer-Based Medical Systems, 392-395 (2004).
20. 陳國禔，微循環顯微觀測系統之設計製作與觀測指標模糊理論應用初
步評估，中原大學醫學工程學系碩士論文，2001。
21. 李錦奎，體表微循環流速量測方法與應用，台灣大學應用力學研究所
碩士論文，2003。
22. 吳至傑，可攜式微血管攝像系統設計與影像分析應用，中原大學電機
工程學系碩士論文，2003。
23. 趙維新，甲襞微循環觀測系統之設計與診斷系統之建立，彰化師範大
學機電工程學系碩士論文，2006。
24. 高耀洁，微循環與健康長壽，中國醫藥報，2000。
25. J. S. Lee, “Biomechanics of the microcirculation, an integrative and
therapeutic perspective,” Annals of Biomedical Engineering 28,
1-13(2000).
26. 陳文傑、田牛、趙國忠，微循環的理論和應用，人民衛生出版社，1987。
27. http://commons.wikimedia.org/wiki/Image:Schema_microscopio.png
28. H. Zhao, R. H. Webb, and B. Ortel, “A new approach for noninvasive
skin blood imaging in microcirculation,” Optics & Laser Technology 34,
51-54 (2002).
29. K. Wolff, L. A. Goldsmith, S. I. Katz, B. A. Gilchrest, A. S. Paller, and
D. J. Leffell, Fitzpatrick's Dermatology in General Medicine, 7th ed.,
McGraw-Hill, 2007.
30. A. Kuramoto, A. Hori, and I. Fujieda, “Laser speckle imaging of a
fingerby scattered light optics,” Piers Online 3, 832-835 (2007).
31. 田牛，微循環方法學，原子能出版社，1985。
32. W Groner, J. W. Winkelman, A. G. Harris, C. Ince, G. J. Bouma, K.
Messmer, and R. G. Nadeau, “Orthogonal polarization spectral imaging:
A new method for study of the microcirculation,” Nature Medicine 5,
1209-1122 (1999).
33. 吳俊霖，使用雙向濾波器之高反差影像自動增強法，科學與工程技術
期刊3，81-87 (2007)。
34. 丁志文，影像處理於SMD 元件定位之應用，台灣科技大學電機工程
研究所碩士論文，2002。
35. Dinrenfass, Blood viscosity, hyperviscosity and hyper-cerebrovascular
disorder, MTP Press, 1986 .
36. 王淑娟，“甲襞微循環檢查及其臨床應用”，中國醫刊12，6-9 (1999)。
37. 繆紹綱編譯，數位影像處理，普林斯頓國際有限公司，2006。
38. 沈俊宏，數位相機上的自動對焦與自動白平衡演算法，台灣大學電信
工程學研究所碩士論文，2006。
39. K. S. Choi, J. S. Lee, and S. J. Ko, “New autofocusing technique using
the frequency selective weighted median filter for video cameras,” IEEE
Transactions on Consumer Electronics 45, 820-827 (1999).