本研究主要設計兩種生物晶片，分別是單一微流道與多重微流道晶片，藉由電滲流作用於不同的親疏水性表面，使DNA拉伸並固定於晶片上，改變傳統只能操作小分子DNA及複雜的蛋白修飾晶片表面來達到固定的效果。實驗晶片主要利用微機電系統技術(Micro-Electro-Mechanical-System, MEMS)製作雙層結構於晶片上(包含金電極層與SU- 8微流道層)，搭配電滲流操作系統與螢光顯微鏡設備作為實驗平台。實驗主要分成三個部份來探討：第一部份是利用DNA本身呈親水性的特性，搭配不同親疏水性的晶片表面，達到較佳的固定DNA環境；第二部份是最佳電壓測試，測試電壓範圍為從0 V開始直到DNA被拉斷為止，電流保持1 mA，以得到拉伸極限電壓；第三部分是拉伸DNA在微流道中，搭配第一與第二部份測試出的拉伸環境進行實驗。實驗結果顯示，以丙酮清洗的晶片最能表現SU-8與玻璃晶片固定DNA的能力，而於單一微流道中施以20 V可拉伸DNA至230 μm；於多重微流道中，DNA會沿著流道拉伸100 μm。由本研究證明利用電滲流拉伸並固定在疏水性的晶片表面，可有效增加拉伸的長度並應用於大型生物分子的操作。

This study designs two types of DNA stretching biochips. One type is single channel microfluidic chip and the other one is microfluidic chip of multiple channels. DNA can be successfully stretched and immobilized on the surface of proposed microfluidic chips. The chip system is fabricated using Micro-Electro-Mechanical-Systems (MEMS) technology, and DNA is stretched utilizing mechanism of Electro-Osmotic Fluid (EOF). The chips were designed by two sides electrodes, between which the gap is 10 mm, which were located under the microchannel to drive EOF and increase the immobilize potential of biochip.The experiment includes three parts: first, the best voltage was determined to stretch DNA. The initial applied voltage is 5 V, and then increase voltage value until DNA break. Second, stretching DNA on the chip surface modified with different hydrophilic materials. Third, stretch DNA on single microfluidic channel chip and multiple channels. The results indicate that the best environment to stretch and immobilize DNA is that use 20 V to drive EOF with the chip washed by Acetone. The maximum stretching length on a single microchannel chip is 230 μm and that on microfluidic chip of multiple channels is 100 μm. The research successfully designed two microfabricated fluidic bio-chips for DNA　stretching and immobilizing

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