在現在日常生活中皆充斥著石化物料的現代，石化能源已是人類日常生活中不可或缺的重要資源，然而隨著現代科技快速且無限制的開發下，石化資源是日漸短絀，加上過度使用石化資源，使得大氣中的碳含量日益增高造成溫室效應，因此，找尋新的替代能源以及減緩溫室效應成為當代人必須解決的首要問題之一，而在中多的替代能源中，在台灣較缺乏地理環境優勢的狀況下，發展生質能源成為台灣可發展的主要替代能源之一。
在本研究中，係以台灣目前一大宗的有機廢棄物─廚餘，以及一具有潛力的生質作物─狼尾草，作為本研究中主要的研究標的物，而在本研究中，是以兩段式的生物程序，去進行有機廢棄物的回收再利用，而本研究係以氫氣以及甲烷氣來回收有機廢棄物中的電子，藉此達到能源再生以及減廢之目的，而廚餘為一富含有機成分之物質(TVS =185 mg/g,-wet weight, moisture=80%)，而其中50%的電子是以油脂為主要型態，而易利用的碳水化合物佔總電子量的16%，而其中揮發酸含量較少，含量最多的乳酸佔總電子量的4%，而次之的醋酸僅有2%。
本研究中，以三個形式相同的I-CSTR作為酸化水解段，串聯一蛋型消化槽作為兩段式操作反應器，而於酸化水解段中，發現於負荷為10 g-COD/L/day時，其有最佳的產氣效益2.47±0.73 mmol-H2/g-COD，而在負荷為14.5 g-COD/L/day時則有最好的產速率0.69±0.21 L-H2/L/day，而負荷為10 g-COD/L/day情況下，所操作的酸化水解槽有乳酸降解之現象發生；而在甲烷消化段，在經過達220天的操作下，於最終穩定時已可操作在55oC，HRT=22天，負荷為4 g-COD/L/day，而其產氣速率可達平均48 mmol-CH4/L/day，而其效益可達16 mmolCH4/g-CODremoval，而在去除效益上可達總COD 80%的去除以及VSS去除率可達70%。
而利用批次可發現於甲烷消化槽中其主要優勢菌群可能是以嗜氫性甲烷菌為主，而在消化槽中雖有丙酸降解之能力，但其對於甲烷產氣的貢獻不甚顯著，而在經過第一段酸化水解之產物於第二段消化處理時須注意其揮發酸過高所造成的抑制現象。
以分子生物結果可發現，於穩定馴養後，相較於初期中溫的優勢菌群其豐富的多樣性，其反應槽體菌相趨於單一，且以Methanosarcina屬之菌群為主要優勢菌。

In contemporary era, petroleum became one of the most important resources in the daily life. With the development of high technology andover-exploiting of petroleum, the absence of petroleum will become a serious problem. The increasing of carbon-dioxide content will contribute in global climate change. Thus, in order to relieve the crisis of energy shortage and prevent the global climate change, The urgency to find out a clean and renewable energy sources become one of the major attention. Despite the disadvantages from a worse geographical environment, development of bio-mass energy seems to be one of renewable energy which Taiwan could develop.
In the present study, a thermophilic two-stages bio-process was conducted to convert the COD of the organic waste into bio-energy. withkitchen waste, which was one of the most abundant waste and the napier grass as the substrates. Kitchen waste contained with high nutritive composition (TVS= 185 mg/g, moisture = 80%). Lipid was the most dominant items in kitchen waste which contained more than 50% of electron while total carbohydrate only about 16%. Electron distribution of VFAs (volatile fatty acids) was about 6% which mainly contributed by lactic acid(4%) and acetic acid (2%).
Three I-CSTR tanks were operated at the first stage and an egg-shaped digester was operated at the second stage. It was found that when volume loading rates (VLR) were designed at10 g-COD/L/day with HRT= 8 days and pH= 6, the best hydrogen yield 2.4±0.73 mmolH2/g-CODadded and the best hydrogen production rate (0.69±0.21 L-H2/L/day) achieved respectively when VLR= 14.5g-COD/L/day. The lactate was degraded obviously when the VLR was 10 g-COD/L/day. In the second stage, an egg-shaped tank was operated as a digester with VLR= 4 g-COD/L/day and HRT= 22 days. The methane production rate could achieve 48 mmol-CH4/L/day under the VLR= 4 g-COD/L/day and the methane yield was 16 mmol-CH4/g-CODremovalrespectively. The removal efficiency of the CODtotal and VSS could reach 80% and 70%, respectively.
The batch test result detected that the dominant archaea might be the hydrogen-utilizing methanogen. The degradation of propionic acid did not contribute to methane production. The concentration of volatile fatty acid from first stage could inhibit the performance of methane production.
By using the scanning electron microscope (SEM), it was found that there were plenty of microbes attached on the organic solids within the egg-shaped digester. And the community structure of microbes in thermophilic state became more simple than mesophilic ones. Methanosarcina became the main group in the egg-shaped digester.

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