58-kDa微小球蛋白（MSP58，也稱為MCRS1），因與核仁蛋白p120的蛋白質交互作用而被首次發現，先前本實驗室的研究表明MSP58可以調節細胞增殖和轉錄。通過酵母雙雜交篩選鑑定MSP58相互作用蛋白，鑑定出兩段獨立的轉錄中間因子 1 γ (TIF1γ)片段，並通過免疫共沉澱證實在轉染後的哺乳動物細胞中MSP58與TIF1γ間相互作用，透過免疫螢光分析MSP58與TIF1γ共同座落在細胞核的位置。TIF1γ與轉化生長因子β (TGF-β)信號通路有關，作為負調節因子發揮作用，並進一步證實了單獨過度表達MSP58會抑制HaCaT和HepG2細胞中TGF-β誘導的 CAGA-Luc報導基因的轉錄活性以及HaCaT細胞中TGF-β下游基因的表達。相反的，內源性MSP58表達的缺失增強了TGF-β誘導的轉錄活性。此外，MSP58和TIF1γ間的協同作用抑制TGF-β介導的轉錄活化。並且，MSP58 表達的降低增強了TGF-β誘導的生長抑制作用。總而言之，本篇的研究表明了MSP58做為TIF1γ的新穎結合蛋白，並在TGF-β通路中扮演負調控的角色。

58-kDa microspherule protein (MSP58, also known as MCRS1) was first discovered by its interaction with nucleolar protein p120. Previous studies in our laboratory have established that MSP58 can regulate cell proliferation and transcription. Using the yeast two-hybrid system to identify MSP58-interacting protein(s), two independent clones encoding the transcriptional intermediary factor 1 gamma (TIF1γ) fragments were identified. Co-immunoprecipitation in transfected mammalian cells confirmed that MSP58 interacts with TIF1γ. Immunofluorescence analysis demonstrated that MSP58 colocalized with TIF1γ in the nucleus. TIF1γ has been implicated in transforming growth factor beta (TGF-β) signaling pathway and plays a role as a negative regulator. We further demonstrated that overexpression of MSP58 alone suppressed TGF-β-induced CAGA-Luc promoter activity in HaCaT and HepG2 cells and TGF-β target genes expression in HaCaT cells. Conversely, depletion of endogenous MSP58 expression enhances TGF-β-induced promoter activity. In addition, MSP58 and TIF1γ synergize in the inhibition of TGF-β-mediated transactivation. Moreover, decreased expression of MSP58 enhances TGF-β-induced growth inhibitory. Taken together, our study suggest that MSP58 is a new interacting partner of TIF1γ and plays a negative role in TGF-β pathway.

吳沛萱，探討微小球蛋白(MSP58)在轉化生長因子(TGF-β)訊息傳遞中所扮演的角色，國立成功大學生物科技與產業科學系碩士論文，2019。
許哲嘉，研究MSP58在調控端粒酶/hTERT和細胞老化中所扮演的角色，國立成功大學藥理學研究所碩士論文，2014。
楊顓賓，探討微小球蛋白(MSP58)調節核醣體基因轉錄的角色與鑑別其核定位及核仁定位序列，國立成功大學生物資訊與訊息傳遞研究所碩士論文，2014。
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