本研究從南台灣篩選出纖維素分解菌株，經16S rRNA序列比對鑑定為Acinetobacter junii F6-02。由纖維素分解酵素(cellulase)及半纖維素分解酵素(xylanase)活性測試發現其酵素活性主要存在胞外。該胞外酵素(主要為xylanase)之最佳反應條件為溫度60oC與pH 7.0。而該菌株生產酵素之最佳培養條件為35oC、曝氣速率0.3vvm、碳源濃度xylan和CMC各5 g/L以及peptone 1 g/L。此菌株所生產之酵素以凍乾方式製備成生物製劑時，其酵素活性優於醱酵上清液，其穩定長期水解之最適溫度為50oC。經由FPLC所純化之xylanase具有兩個活性部位其分子量分別約為70與85 kDa。而在酵素動力學探討方面對於xylan和氫氧化鈉鹼處理稻桿之vmax及Km值分別為8.6 g/L/h, 10.6 g/L和3.6 g/L/h, 26.9 g/L。以xylanase生物製劑水解xylan與鹼前處理稻桿所得之還原糖中有超過90%的木糖，顯示優異的水解效果。本研究應用Clostridium butyricum CGS5為菌株，xylan水解液和鹼前處理稻桿之水解液為料源之產氫速率分別為62.5與26.8 ml/h/L，而產率分別為0.70 與 0.76 mol H2/mol xylose。一階段的同步xylan酵素水解糖化發酵產氫在37oC之產氫速率為35.3 ml/h/L，顯示產氫之效果不如二階段水解產氫程序。

A cellulolytic bacterial strain was isolated from soil taken from southern Taiwan and identified as Acinetobacter junii F6-02 via phylogenetic and 16S rRNA sequencing analysis. Investigation of cellulases and xylanase production of Acinetobacter junii F6-02 showed that the activity of those cellulolytic enzymes were mainly located extracellularly. The optimal temperature and pH for xylanase activity 60oC and 7, respectively, while the best condition for xylanase production by A. junii F6-02 was 35oC, and 0.3 vvm aeration speed was the best xylanase production condition. The best carbon source concentration and nitrogen source to optimize xylanase production was 5 g/L each for CMC and xylan and peptone 1 g/L. Lyophilized enzyme bioagent produced from Acinetobacter junii displays better xylanase ability than supernatant of the fermentation broth. The enzyme bioagent displayed a stable long-term hydrolysis at a temperature of 50oC. Xylanase purification via FPLC shows that two xylanase enzymes exist in the supernatant of Acinetobacter junii and their molecular weight is about 70 and 85 kDa. The vmax and Km of xylanase with the substrate of xylan and NaOH pretreated straw was 8.6 g/L/h, 10.6 g/L and 3.6 g/L/h, 26.9 g/L, respectively. Batch H2 fermentation with Clostridium butyricum CGS5 shows that using hydrolysate of xylan and NaOH pretreated rice straw as the substrate, the maximum H2 production rate was 62.5 and 26.8 ml/h/L, respectively, and maximum H2 yield were 0.70 and 0.76 mol H2/mol xylose, respectively. Simultaneous enzymatic xylan saccharification and hydrogen fermentation was also investigated. Under a reaction temperature of 37oC, the hydrogen production rate reached a maximum value of 35.3 ml/h/L, which is markedly lower than that obtained from the two-stage process.

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