多氯戴奧辛（PCDDs）及多氯呋喃（PCDFs）為一系列含鹵素之芳香族化合物，是化學工業製程及燃燒過程中產生的副產物。其穩定性及脂溶性極高，為具高毒性之環境汙染物，可經由食物鏈生物累積的方式進入人體，進而危害人體的健康。而在許多種PCDD/Fs之異構物中，只有17種在2,3,7,8位置有氯鍵結之異構物具較強之毒性。
本研究主要目的在探討不同層級生物體內PCDD/Fs之濃度特徵分佈與環境之相關性，藉由分析食物鏈中低層級生物至高層級生物(即牡蠣、虱目魚及豬)體中PCDD/Fs的含量，來了解這些生物體內戴奧辛的濃度、指紋區的分佈及累積方式，並與相關文獻比較，進行一系列之探討。本研究之樣本來自台灣南部，分析方法依據環檢所公告方法NIEA M801.11B及美國環保署公告方法USEPA M1613B之前處理步驟及儀器分析方法執行，所有相關品管、品保措施，亦均依循方法訂定之規範。前處理流程包含索氏萃取、燒瓶式除脂、酸性矽膠、酸性氧化鋁及活性碳/矽藻土管柱淨化，再以高解析度層析質譜儀進行分析。
研究結果顯示，牡蠣中PCDD/Fs的總量濃度較低，豬肉中PCDD/Fs的總量濃度最高。由於豬屬於食物鏈中高層級之生物，因此具有較高之累積量，其次是虱目魚及牡蠣，且其高氯數之PCDD/Fs亦佔較高之比例。但高氯數之PCDD/Fs所貢獻之毒性當量濃度較小，故其體內所累積之毒性也較小。而牡蠣為定著性之生物，故體內PCDD/Fs之濃度分佈象徵局部養殖區汙染之狀況。本研究及相關文獻比較，發現台灣環境之趨勢影響著牡蠣體內PCDD/Fs之特徵分佈。

Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are series of chlorinated aromatic homologs which are unwanted by-products generated in the processes of combustions and industrial chemical manufacturing. These compounds are extremely stable and very lipophilic, and PCDD/Fs are both considered to be highly toxic environmental contaminants. PCDD/Fs can be accumulated via the food chain and enter the human body to induce adverse health effects. Among the many PCDD/Fs isomers, there are 17 species of the 2,3,7,8-chloro substituted congeners are regarded as having the most severe toxicity.
In this study, the characteristics of the toxic PCDD/Fs in tissues of different animal species are investigated and correlation ship of the results and the environment are also studied. Three animal species in range from the lower to higher levels of the food chain, such as oysters, milk fishes, and porks, were analyzed for the PCDD/Fs. The concentrations of each congener and the PCDD/Fs distribution pattern in these species were closely examined and compared with the findings in various literature. Discussions are focused on the fates of the PCDD/Fs in the animal species as well as in correlating the distribution pattern with the environment. The samples used in this study were collected in southern Taiwan. The official analytical method of Taiwan for dioxins and furans, NIEA M801.11B and the USEPA method 1613B, were employed throughout the entire analytical procedure, including sample preparation and instrument analysis. All quality control and quality assurance procedures were also strictly adhered to as described in the methods. Preparation methods included Soxhlet extraction, lipid elimination, and acidic silica gel/acidic alumina/carbon/celite column clean up procedures, and the final sample extracts were analyzed by utilizing high-resolution gas chromatography/ high-resolution mass spectrometry (HRGC/HRMS).
The investigation results indicated that in addition to the total PCDD/Fs concentration, the fraction containing the highly chlorinated PCDD/Fs also showed an increasing trend from oysters, milk fish to pigs. Since porks are higher up the food chain than milk fishes and oysters, they are expected to have a higher accumulation of PCDD/Fs. The analytical data paralleled well with the expectation. However, the highly chlorinated PCDD/Fs have relative low toxicity equivalent factors compared to the less chlorinated congeners; the contribution to the toxicity in the body is less significant. Oysters are a sessile animal species; thus, the PCDD/Fs concentrations and distribution patterns found in the oyster normally reflect the contamination of the farming environment. It was also found that the PCDD/Fs distribution pattern found in the oyster samples is likely affected by the environment of Taiwan.

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戴奧辛及呋喃檢測方法—同位素標幟稀釋氣相層析/高解析質譜法， NIEA M801.11B，中華民國94年11月30日環署檢字第0940097070號公告