在所有榖類植物中稻子是唯一可以在缺氧的狀態下發芽的植物，但在發芽的過程中因為缺氧的關係而會抑制根的生長。經測試後我們發現稻子本身在缺氧1天、2天和3天的狀態下會分別釋放出大約4mM、8mM和12mM濃度的酒精到水中，而稻子芽鞘組織內缺氧十小時酒精濃度就可達到7mM，時間越久所產生的濃度就越高。所以我們在有氧的狀態下加入不同濃度的酒精，發現在酒精濃度超過425mM就會完全抑制根的生長，顯示酒精的釋放應具有重要的生理調節功能。所以我們根據這個生理現象我們對市售酵素alkaline phosphatase（ALP）、aldolase和glucose-6-phosphate dehydrogenase（G-6-PDH）的酵素反應進行酒精影響試驗，而根據我們所做的實驗得知在沒有和酒精預先培養下在5mM的酒精濃度對於ALP、aldolase和G-6-PDH速率分別增加11﹪、9%和21﹪，在20mM的酒精濃度下ALP和G-6-PDH速率分別增加了11﹪和21﹪，而在有預先培養下在5mM酒精濃度下ALP、aldolase和G-6-PDH速率分別增加了20﹪、15﹪和43﹪，在20mM酒精濃度下ALP和aldolase速率分別增加了28﹪和48﹪。顯示了受測的酵素在上述生理反應下的酒精濃度下均有明顯的改變，且在有預先培養下酒精的影響會比沒有預先培養來的大。由我們這個前導性的實驗，我們推測酒精對於稻子體內的酵素可能具有同樣的功能，以利在缺氧的狀態下繼續提供能量供稻子生長，如果要證實上述的想法我們要真正從稻子裡萃取出酵素，而真正的做到in vitro的實驗並對於稻子在缺氧狀態下進行發芽嘗試解答酒精的功能。

Rice is the only major crop plant that can germinate well under anaerobic condition. But rice seedlings germinate under anaerobic condition, deficiency of O2 can inhibit rooting. After we tested, we found that rice coleoptiles under anaerobic condition would release ethanol into media 4mM at 1 day, 8mM at 2 day and 12mM at 3 day respectively. The concentrations of ethanol in rice tissues that germinated anaerobically at 10 hours would exceed 7 mM. It showed that rice released ethanol should have important function of physiological regulation. We added different concentrations of ethanol, ethanol concentrations exceeded 425 mM and the roots of rice seedlings would inhibit completely. According to this physiological phenomenon, we proceeded the effect of ethanol to enzyme’s reaction for commercial enzymes, alkaline phosphatase(ALP) that come from rabbit muscle, aldolase that come from rabbit muscle and glucose-6-phosphate dehydrogenase(G-6-PDH) that recombinant in E. coli. Without pre-incubation, in 5mM ethanol the rate of increasing could reach 11%, 9% and 21% to ALP, aldolase and G-6-PDH respectively and in 20mM ethanol the rate of ALP and G-6-PDH increase 11﹪and 21﹪. With pre-incubation, the rate of increasing could reach 20%, 15% and 43% to ALK, aldolase and G-6-PDH in 5mM ethanol respectively and in 20mM the rate of ALP and aldolase increase 28﹪and 48﹪. We found that the enzyme activity treated with ethanol had more activity than un-treated enzyme. From our leading experiment, we guess that ethanol may have the same effect in rice enzyme and supply energy to cell growth continuously. If we want to provide the thinking, we must extract enzyme from rice tissue and try to elucidate the functional role of ethanol when rice germinate under anaerobic condition.

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