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研究生: 郭智宇
Kuo, Chih-Yi
論文名稱: 橙黃壺菌BL10之多元不飽和脂肪酸合成酶的偵測與分析
Detection and analysis of polyunsaturated fatty acid synthase in Aurantiochytrium strain BL10
指導教授: 陳逸民
Chen, Yi-Min
學位類別: 碩士
Master
系所名稱: 生物科學與科技學院 - 生物科技與產業科學系
Department of Biotechnology and Bioindustry Sciences
論文出版年: 2021
畢業學年度: 109
語文別: 中文
論文頁數: 69
中文關鍵詞: 橙黃壺菌 BL10多元不飽和脂肪酸合成酶免疫共沉澱法高分子量蛋白質分析
外文關鍵詞: Aurantiochytrium BL10, PUFA, Immunoprecipitation, High MW protein analyze
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  • 中文摘要 I 英文摘要 II 誌謝 VI 目錄 VII 圖目錄 X 附表目錄 XII 縮寫表 XIII 一、研究背景 1 1-1 BL10之多元及高度不飽和脂肪酸的生成 1 1-2 BL10之多元及高度不飽和脂肪酸生合成的調控 1 1-3 BL10之多元及高度不飽和脂肪酸合成酶的研究 4 1-4 研究目的 9 二、材料與方法 10 2-1 重要藥品、儀器與耗材 10 2-2 AmPFA1 的western blot分析方法的建立 12 2-3 AmPFA萃取方法的優化 14 2-4 AmPFA含量在不同時間下之變化 16 2-5 AmPFA完整性的分析 17 2-6 AmPFA1異構體序列與分子量的分析 19 2-7 AmPFA1異構體的轉譯後修飾分析 20 2-8 AmPFA1異構體Time Crouse的分析 21 三、結果 22 3-1 AmPFA1 的western blot分析方法的建立與抗體之選定 22 3-2 AmPFA萃取方法的優化 22 3-3 AmPFA含量在不同時間下之變化 23 3-4 AmPFA完整性的分析 24 3-5 AmPFA1異構體序列與分子量的分析 25 3-6 AmPFA1異構體轉譯後修飾的鑑定 26 3-7 AmPFA1異構體Time Crouse的分析 27 四、討論 28 參考文獻 32 圖表 36 附錄 61

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