本篇研究論文，為利用熱泳效應可用來操控分子並且定量分子之特性，結合與特定的目標分子具高度專一性的核酸適體，發展靈敏且快速的檢測方法。許多的研究文獻已指出熱泳可應用於聚集及分析微流體中的微粒子及寡核苷酸。其原理為當給予一熱源，中心點為加熱區而周圍則為冷區域，流體中的微粒子及寡核苷酸會沿著熱源所形成的溫度梯度差而移動。而移動的方向及速率與索瑞特擴散係數有關。微粒子及寡核苷酸的構造、大小、電荷、溶液酸鹼值等等，都是影響熱泳效應的參數。因此，可以藉由改變這些參數來操控微粒子的移動。本研究的目的主要為聚集並且定量丙型干擾素。當人體內的T淋巴球再度暴露在結核分支桿菌抗原下，會釋放出丙型干擾素。丙型干擾素為一種細胞激素，會刺激各種免疫細胞進行一連串的免疫反應。因此，為了潛伏性肺結核篩檢的應用，本研究利用核酸適體來捕捉丙型干擾素，然後利用熱泳效應聚集核酸適體以進行丙型干擾素的定量。由於核酸適體與丙型干擾素結合前後會有結構上的變化，所以兩者的熱泳效應也會有所不同。核酸適體受熱泳效應而聚集的程度會隨著丙型干擾素的濃度而有所變化。研究結果顯示我們可以依據熱泳所造成的聚集程度來定量丙型干擾素，過程中不需要任何繁複的表面修飾與沖洗步驟。

In this research thesis, we utilized the abilities of thermophoresis for molecular manipulation and quantification. And we aslo utilized highly specific aptamers with particular target molecules. To develop a sensitive and rapid detection method based on thermophoresis. Numerous reseach literatures have indicated that thermophoresis effect could be applied to accumulate and analyze particles and oligonucleotides in the microfluidics. The principle of thermophoresis effect is that when providing a heat source, the center is the heating zone while the periphery is colder region, particles and oligonucleotides in the microfluidics would migrate along the temperature gradient formed by a heat source. While the direction and rate of migration were related to the soret coefficient. And particles and oligonucleotides structure, sizes, charge, pH of solution, etc., would affect the thermophoresis effects. Therefore, we could manipulate particles and oligonucleotides by altering these parameters. The main purpose of this study was the aggregation and quantitation of interferon-gamma. When human body T cells once again exposed to the antigens of Mycobacterium tuberculosis, they would stimulated to release interferon-gamma. Interferon-gamma is a kind of cytokines that it could stimulate a series of immune cells to carry out the immune responses. Therefore, for applications of latent tuberculosis screening, this study utilized aptamers to capture interferon-gamma. Then made use of thermophoresis to accumulate aptamers for quantification of interferon-gamma. Because there were structural changes in aptamers binding to interferon-gamma or not, the thermophoresis effects of both would be different. Level of accumulation aptamers varied due to concentration of interferon-gamma. The results showed that we can quantify interferon-gamma on the basis on the level of accumulation caused by thermophoresis effect. And the process does not require any complicated surface modification and washing steps.

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