人體尿蛋白在臨床的腎臟病診斷時扮演重要角色，尿蛋白的檢測可以協助醫護人員判斷腎臟功能及其疾病的嚴重程度。本研究中擬製備電化學尿蛋白感測器，主要利用循環伏安法及交流阻抗分析法探討不同濃度、時間及酸鹼值下Fe(CN)63- / Fe(CN)64- 系統的峰電位、峰電流以及阻抗的變化，並利用自組裝單層膜修飾金電極提高靈敏度。
實驗結果顯示，利用N-(3-Dimethylaminopropyl)-N’-ethylcarbod- iimide hydrochloride ( EDC )及N-hydroxysuccinimide ( NHS ) 穩定電極表面的修飾層是可行的，以自組裝單層膜（SAM）修飾金電極與裸電極比較，電極在修飾前後皆為不可逆電化學反應，峰電位差及峰電流比率隨著吸附時間而改變，40分鐘後趨於穩定。隨著白蛋白濃度提高，不可逆性上升、反應活性變小，峰電位差對白蛋白的靈敏度從裸金電極的6.260 mV/ppm提升到SAM電極的7.398 mV/ ppm。而峰電流比率對白蛋白的靈敏度從裸電極的1.324 ppm-1（氧化）及1.506 ppm-1（還原）提升到SAM電極的1.703 ppm-1（氧化）及1.831 ppm-1（還原）。Rs及Rct 也隨著濃度及吸附時間成上升趨勢。

Human urinary albumin plays an important role in the clinical diagnosis of kidney disease. The examination of urine albumin can help medical staff determine kidney function and seriousness of disease. In this study, an electrochemical urinary albumin sensor was proceduced. It mainly used cyclic voltammetry and ac impedance analysis to detect peak potential, peak current and impedance in different concentrations and adsorption times of albumin, and pHs of electrolyte in a Fe(CN)63- / Fe(CN)64- system. SAM modified electrodes were also applied to increase sensitivity of the detection.
The result shows the feasibility by using EDC and NHS to stabilize the modification layer of electrode surface. According to the results from the SAM modified and bare electrodes, the reactions on electrodes both before and after modification were all irreversible eletochemical reaction. Peak potential and peak current ratio changed with adsorption time and became stable after 40 minutes. The sensitivity of peak potential difference was 6.260 mV/ppm with bare electrode, and was improved to 7.398 mV/ ppm with SAM electrode. When albumin concentration increased, the irreversibility increased and activity of electrode decreased. The sensitivity of peak current ratio was 1.324 μA/ppm (oxidation) and 1.506 μA/ppm (reduction) with bare electrodes, and was improved to 1.703 μA/ppm (oxidation) and 1.831 μA/ppm (reduction) with SAM electrodes. Rs and Rct increased with the albumin concentration and adsorption time.

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