許多蟬種會在黎明與黃昏進行密集而短暫的合唱，這種現象稱為晨昏合唱。北埔蜩(Taiwanosemia hoppoensis)為台灣特有蟬種，一天中僅於黎明與黃昏進行短暫的合唱，其鳴叫活動顯然屬於生物週律的現象。本研究第一部分是描述台南市北埔蜩族群的晨昏合唱模式，並分析合唱起訖時間，與日出日落時間、雲量、環境照度、氣候因子以及合唱族群大小間的相關性，藉此找出可能影響合唱模式的因子。第二部分則是在持續黑暗與持續微光的處理下觀察北埔蜩個體的鳴叫時間，以了解光週期對誘發鳴叫活動的重要性以及鳴叫活動是否具有內生性的機制。於2001年在台南市海岸防風林之研究，發現北埔蜩晨昏合唱的季節為五月初至八月中。黎明合唱始於04:23~04:53，終於04:55~05:15，合唱持續時間平均為24.6 min，合唱強度平均為59.9 dB。黃昏合唱始於18:19~18:55，終於19:00~19:35，合唱持續時間平均為37.7 min，合唱強度平均為62.9 dB。同年在防風林中畫設樣方並進行蟬蛻移除法，發現北埔蜩成蟲的羽化由5月初開始，於6月中下旬達到高峰，至8月初結束，雄雌性比為1 : 1.07，蟬蛻密度為3.08 m-2。統計分析顯示，北埔蜩族群之晨昏合唱模式主要與環境照度及合唱族群大小有顯著相關。此外，在持續黑暗與持續微光下，北埔蜩個體表現晨昏鳴叫的機率顯著小於正常光週期下表現之機率，此結果並不支持內生性週期的存在，而光強度的變化才是誘發北埔蜩個體鳴叫的主要機制。

　　Taiwanosemia hoppoensis (Matsumura 1907) (Homoptera: Cicadidae) chorus specifically in the dawn and dusk. This behavior can be regarded as a biological rhythm. Previous studies on other crepuscular cicadas suggested that light intensity is the exogenous cue inducing crepuscular chorus, but this remains unverified. In the first part of this study, the crepuscular chorus pattern of T. hoppoensis in the coastal windbreak forest of Tainan, Taiwan, was described and the number of exuviae were estimated as an index of chorus population size. The crepuscular chorus of T. hoppoensis occurred from early May to mid August. The dawn chorus started at 04:23~04:53, finished at 04:55~05:15, and lasted for 24.6 min on average. The mean chorus intensity of dawn chorus was 59.9 dB. The dusk chorus started at 18:19~18:55, finished at 19:00~19:35, and lasted for 37.7 min on average. The mean chorus intensity of dusk chorus was 62.9 dB. The emergence of adults began in early May, peaked on ca. 20, June, and finished in early August. Sex ratio and average density of the exuviae were 1 : 1.07 (♂ : ♀) and 3.08 m-2, respectively. Analyses of onset and cease time of the chorus by step-wise multiple regression indicate that the chorus population size and the ambient light intensity are the important determinants. In the second part of this study, individual male was treated with natural photoperiod, constant dark, or constant twilight to test the existence of endogenous rhythm controlling the calling activities. Proportions of crepuscular calling individuals in the constant dark and twilight groups were significantly lower than that in the normal photoperiod group. Besides, abrupt light-off induced calling of the males. These results do not support the presence of an endogenous rhythm and suggest that the change of light intensity is the main exogenous cue for calling of T. hoppoensis.

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