近年來伴隨著奈米科技的發展，磁性奈米材料廣泛應用於生物醫學領域。由於其獨特的超順磁特性，藉由外加磁場控制，磁性奈米粒子可作為體內標靶的載體，再連接上具專一辨識性之抗體免疫球蛋白，可較精準地標靶至病灶所在位置，以至抑制控
制病情，甚至治癒，因此在生物醫學上相當具有研究與臨床實用價值。
本研究以Fe(CO)5當作前驅物，利用有機相熱裂解法製備超順磁性鐵氧奈米粒子(Fe3O4@OA/OC)，在其外層包覆3-aminopropyltriethoxysilane (APTES)，以配位交換法改質成為親水相，而最後在表面接上糖化血紅蛋白抗體 (Anti-HbA1c)，針對監控糖尿病的主要單元糖尿血紅蛋白 (hemoglobin A1c, HbA1c) 具有標靶專一吸附作用。研究過程中以X光繞射儀 (X-ray diffraction) 證實奈米磁性粒子為Fe3O4@OA/OC之晶相並估算其平均微晶尺寸；以傅立葉轉換紅外線光譜儀 (Fourier transform infrared spectroscopy, FT-IR)、熱重分析儀 (thermogravimetric analyzer, TGA) 及能量散射光譜儀 (energy dispersive spectrometer, EDS) 確認磁性粒子表面改質後無機矽烷的包覆；利用紫外光/可見光光譜儀 (UV/Vis spectrophotometer) 判斷Anti-HbA1c 是否修飾於親水性磁性粒子表面；以穿透式顯微鏡 (transmission electron microscope, TEM) 分析觀察Fe3O4@OA/OC、Fe3O4@APTES，以判斷矽烷配位交換反應的細節對於奈米粒子尺寸的影響，並估算平均粒徑分別為4.7± 0.7nm及10.6 ± 1.3nm；由超導量子干涉儀 (superconducting quantum interference device, SQUID) 所得之磁滯曲線得知Fe3O4@OA/OC及Fe3O4@ATPES的飽和磁化量分別為 39.73 emu/g與 25.23 emu/g，並且二者皆具有超順磁的性質，顯示在超順磁材料上披覆無機物質並不影響其磁性質。
在醫療應用上，以Anti-HbA1c進行包覆，經由抗體包覆的磁性粒子可標靶至糖化血紅蛋白，並利用高效能液相層析儀 (high performance liquid chromatography, HPLC)
進行分離鑑定，作進一步監測糖尿病之功效。

In recent year, the nanotechnology have been applied in many fields like biomaterial and medical. Especially in magnetic nanoparticles, according to its specific property ‘superparamagnetic’, the magnetic nanoparticles would play an important role as drug carrier to do the in vivo diagnosis. Magnetic nanoparticles would attracted by external magnetic field to the lesions, and it would be more specific if the particle surface binding with active targeting ligand like drug or antibodies.
In this research, the magnetic nanoparticles were synthesized by thermal decomposition to form Fe3O4@OA/OC nanoparticles, then modified by 3-aminopropyltriethoxy silane (APTES) to make the nanoparticles surface covered of amino group to enhance hydrophilicity. Finally we conjugated anti-HbA1c on nanoparticles in order to target HbA1c antigen to monitor diabetes.
Transmission electron microscopy (TEM) images reveals that Fe3O4¬@OA/OC were monodisperse in n-hexane, and its diameter was estimated to be 4.7± 0.7nm, after APTES modified the particles aggregated, the Fe3O4@APTES diameter enlarged to 10.6± 1.3nm. X-ray diffraction (XRD) analysis confirmed that the crystalline plane is the same between Fe3O4@OA/OC & Fe3O4@APTES, thermogravimetric analysis (TGA) observed the weight loss to make sure that APTES were successfully modified on particle surface, Fourier transform infrared spectroscopy (FT-IR) could also approve the fact by the peaks different between Fe3O4@OA/OC and Fe3O4@APTES, finally the magnetization is analyzed by superconducting quantum interference device (SQUID), the saturated magnetization of Fe3O4@OA/OC and Fe3O4@APTES were 39.73 emu/g and 25.23 emu/g respectively.
In biofunctional magnetic nanoparticles section, Fe3O4@APTES conjugated with Anti-HbA1c (Anti-Fe3O4) were identified by FT-IR, there was a special peak shown on the spectrum at 400-500 cm-1 represented antibodies S-S bond. The supernatant which magnetic nanoparticles immune with antibodies was analyzed by ultraviolet-visible (UV/Vis), the results shown that about 4.438 mg Anti-HbA1c conjugated on nanoparticles surface. Finally we did an in vitro test to observe whether the biofunctional particles will traced HbA1c or not, the results of HPLC approved that Anti-Fe3O4 could successfully target to HbA1c, so the biofuctional magnetic nanoparticles could further monitored diabetes patients’ blood HbA1c concentration.

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