利用免疫染色的方式來偵測Fos蛋白質的表現及其活化情形，進而去標的特殊因應刺激而反應的神經元的方式，已被許多研究證實是可行且有效的。儘管我們已經知道甲基安非他命所引發的場地制約偏好反應是一個長期的記憶，但是我們對於此記憶在中樞神經系統是如何存放及提取，以及在哪裡存放和提取等都尚未明瞭。在本實驗中，我們以免疫染色的方式，偵測甲基安非他命所引發的場地制約偏好反應之記憶在提取後，邊緣系統中Fos蛋白質表現的變化。和以往的研究所不同的是，本實驗用以使動物提取記憶的方式並不是透過讓動物去表現該偏好記憶，而是將動物放至和當初甲基安非他命配對的類似環境中，利用這些環境線索的相似性來誘發其場地制約偏好反應之記憶。我們發現這樣的實驗設計可以有效地建立起場地制約偏好反應之表現及記憶，同時我們也發現當該記憶被提取時，在大腦內側前額葉皮質及伏隔核的核心部份Fos蛋白質的量都有顯著的上升情形。此外，我們也發現熟悉環境線索的刺激亦可造成大腦紋狀體背側以及齒狀迴的Fos蛋白質表現量上升。而伏隔核的外殼部份及杏仁核的側底核和側核部分，似乎並不參與在甲基安非他命引發的場地制約偏好反應之記憶的儲存和提取。綜合上述的結果，我們認為在邊緣系統中，內側前額葉皮質及伏隔核的核心部份參與在甲基安非他命引發的場地制約偏好反應之記憶的儲存和提取，而此兩個腦區為就解剖層面探討甲基安非他命成癮相關記憶之關鍵位置。

Immunohistochemical Fos staining has proved to be a method to identify the loci of activated neurons specific to a stimulus. Although the methamphetamine (MA)-conditioned place preference (CPP) is a long-term memory, how and where the MA-CPP memory was stored and retrieved remained unknown. In this study, a conditioning procedure comprising of vehicle (saline) injection and previously-conditioned environment presentation, not performance test, was used as a way to reactivate the MA-CPP memory. Activated neurons in the limbic system following this MA-CPP memory reactivation were examined by employing immunohistochemical Fos staining. We demonstrated that the current conditioning procedure produced reliable MA-CPP memory and performance. Moreover, enhanced Fos expressions were obviously found in medial prefrontal cortex and the core of nucleus accumbens while the MA-CPP memory was reactivated. Different from many findings, familiarity with environmental cues/context in a novel surrounding (the CPP chamber) was found to significantly enhance neuronal activity in dorsal part of the striatum and the dentate gyrus. Nucleus accumbens shell, basolateral or lateral amygdala, in this regard, did not seem to be involved in the MA-CPP memory storage or retrieval. These results, taken together, suggest that medial prefrontal cortex and nucleus accumbens core are anatomical substrates responsible for storage and reactivation of the MA-conditioned cues/context-associated memory in the limbic system.

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