結直腸癌是眾所周知的高死亡率的文明疾病之一。 它是男性和女性中第三大最容易診斷的癌症，也是第二大致命的惡性腫瘤。 IRSp53也稱為brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2)。 它有四種正在研究的IRSp53異構體（T、S、M、L）。 許多報告表明 IRSp53介導肌動蛋白重組。Eps8 (Epidermal Growth Factor Receptor Pathway Substrate 8) 是結直腸癌中的一種致癌蛋白。在早期的研究中，我們發現IRSp53-M蛋白促進了小鼠皮下腫瘤的形成，並且IRSp53-M蛋白進一步增強了細胞的侵襲、細胞遷移。 為了了解 IRSp53 異構體在癌症轉移中的重要性，我們對小鼠進行了腹膜內注射結腸癌細胞。 我們分析了(SW620、SW480、HCT116、WiDr)中IRSp53異構體的mRNA表現量，SW620中IRSp53-M的表達高於其他IRSp53異構體。 我們觀察到SW620、HCT116和HT29細胞，它們是IRSp53-M和T過表達細胞，在小鼠中形成腫瘤，但沒有觀察到SW480細胞形成腫瘤。在soft agar assay中，IRSp53異構體不同程度地促進細胞集落形成。 此外SW480細胞中的IRSp53-M、T蛋白增強了腫瘤的形成，並且我們發現表達IRSp53的細胞中巨噬細胞滲入腫瘤組織。 除此之外，也利用共聚焦顯微鏡新 IRSp53 異構體在調節 F-肌動蛋白聚合和細胞膜定位中的作用，總而言之，這些數據表明IRSp53對於細胞生長、癌化、促成腫瘤生長很重要。

Colorectal cancer is one of the well-known diseases of civilization with a high fatality rate. It is the third most easily diagnosed cancer and the second most deadly malignant tumor in both male and female. IRSp53 is also called brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2). It has four well study isoforms (T, S, M, L). Many reports indicate IRSp53 mediate actin reorganization. Eps8 (Epidermal Growth Factor Receptor Pathway Substrate 8) is an oncogenic protein in colorectal cancer. In earlier studies, we found that M form promoted tumor formation in the subcutaneous of mice, and M form further enhanced cell invasion, cell migration. To understand the important of IRSp53 isoforms in cancer metastasis, we performed an intraperitoneal inoculation of colon cancer cells in mice. We analyzed mRNA level of IRSp53 isoforms in (SW620, SW480, HCT116, WiDr), the expression of IRSP53-M in SW620 was more than other IRSp53 isoform. We observed SW620, HCT116 and HT29 cells, which are M- and T- overexpressing cells formed tumor in mice, but not SW480 cells. In soft agar, IRSp53 isoform promoted colonies formation in various degree. Moreover, IRSp53 M, and T-form in SW480 cells enhanced tumor formation, and we found that macrophage recruitment in expressing IRSp53 cells. In addition to this, confocal microscopy is used to investigate IRSp53 isoforms in regulating F-actin polymerization and plasma membrane localization. Conclusion, these data demonstrated that IRSp53 is important to regulate cell proliferation in colon cancer formation.

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