肌酸酐 (creatinine) 為肌肉中肌酸 (creatine) 代謝而來的產物，為人體腎臟代謝循環的最終產物，同時也是診斷人體腎功能的重要指標。本實驗利用4-溴基-1,8奈基酸酐 (4-bromo-1,8-naphthalic anhydride) 經開環取代反應及芳香族官能基取代反應合成螢光功能性單體4-甲胺基-1,8丙烯基奈基醯亞胺 (4-methylamino-N-allylnaphthalimide) 作為功能性單體，與另一功能性單體甲基丙烯酸 (methacrylic acid)，在交聯劑乙二醇二甲基丙烯酸酯 (ethylene glycol dimethacrylate) 作用下，進行高分子聚合反應製備肌酸酐螢光模版高分子。
肌酸酐模版高分子在單成份肌酸酐溶液吸附中，對肌酸酐之吸附量為1.821 ± 0.067 mg creatinine/g MIP，而無模版高分子對肌酸酐之吸附量為0.502 ± 0.026 mg creatinine/g NIP，兩者相除所得之模印因子為3.632 ± 0.332。在相似物的選擇性吸附中，雙成份系統裡對共存物肌酸 (creatine)以及相似物N-羥基丁二醯亞氨 (N-hydroxysuccinimide)、及咯烷酮 (2-pyrrolidinone) 的選擇率分別為4.776 ± 0.892、7.636 ± 1.273以及2.538 ± 0.393，可證明模版高分子具有良好的辨識能力。在血清的干擾性吸附中，模版高分子對肌酸酐之吸附量為1.481 ± 0.278 mg creatinine/g MIP，而無模版高分子對肌酸酐之吸附量為0.443 ± 0.013 mg creatinine/g NIP，模印因子為3.335 ± 0.533，可知模版高分子即使在干擾物較多的血清環境下仍可保有良好的辨識能力。
以螢光光度計檢測螢光模版高分子之相對螢光強度變化量對肌酸酐濃度變化實驗中，螢光模版高分子對螢光無模版高分子之模印因子為4.142 ± 0.288。而對共存物肌酸 (creatine) 以及相似物N-羥基丁二醯亞氨 (N-hydroxysuccinimide)、及咯烷酮 (2-pyrrolidinone) 的選擇率則為3.222 ± 0.081、5.800 ± 0.067以及2.231 ± 0.051，相較於前述之吸附實驗所得的選擇率稍差，可能是檢測方法不同之故，亦有可能是因為吸附條件不同的影響，故未來尚須尋求更佳的螢光檢測方法或條件來提升選擇率，以求更精準的螢光模版高分子感測效果與實用性。

Creatinine is decomposed from creatine in muscle and is one of the final products of kidney metabolism. It is used as an important index to evaluate the kidney function. 4-Bromo-1,8-naphthalic anhydride was used to synthesize 4-methylamino-N-allylnaphthalimide, which is a fluorescent functional monomer. It was co-polymerized with another functional monomer, methacrylic acid, in the presence of crosslinker, ethylene glycol dimethacrylate and creatinine template molecule, to obtain the imprinted fluorescent polymer.
The binding capacity of the imprinted fluorescent polymer toward creatinine in creatinine solution was 1.821 ± 0.067 mg creatinine/g MIP. A capacity of 0.502 ± 0.026 mg creatinine/g NIP can be obtained from the corresponding non-molecularly imprinted polymer (NIP). The ratio of the binding capacity for MIP to that for NIP is defined as imprinted factor. In this case, the imprinted factor was 3.632 ± 0.332. For selective absorption, the analogs used were creatine, N-hydroxysuccinimide and 2-pyrrolidinone. The resulted selectivity ratios were 4.776 ± 0.892, 7.636 ± 1.273 and 2.538 ± 0.393, respectively. In serum test, the binding capacity were 1.481 ± 0.278 mg creatinine/g MIP and 0.443 ± 0.013 mg creatinine/g NIP respectively. The imprinted factor from serum was 3.335 ± 0.533. Hence, serum did not seem to cause severe interference in this case.
As for the fluorescent detection, the correlation between the quenched amount of fluorescent intensity and the creatinine concentration was used to calculate the imprinted factor. The imprinted factor was 4.142 ± 0.288. Additionally, the selectivity ratios for creatine, N-hydroxysuccinimide and 2-pyrrolidinone were 3.222 ± 0.081, 5.800 ± 0.067 and 2.231 ± 0.051, respectively. As a result, the preliminary test on the selective detection of creatinine by using imprinted fluorescent polymers was successfully performed.

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