至 2006 年，台灣廚餘的回收率已可達三分之一，日回收量超過 1,500 噸。現行的廚餘回收再利用的方式以養豬（75%）及堆肥（22%）為主，但這兩種回收再利用的方式皆有衛生環境上的隱憂，例如傳染疾病以及二次污染之虞。在另一方面，由於台灣缺乏化石燃料（煤礦、石油、天然氣等）的來源，98% 以上的能源來源必需仰賴進口，更有迫切開發替代性能源之需求。基於環境與能源這兩者的共同考量，我們期望透過發展廚餘厭氧醱酵，利用此對環境較為友善的方法來達到有機廢棄物的減量，並且開發潔淨生質能源的技術平台。
　　廚餘是一種高濃度的有機廢棄物，其總 COD 約 82-107 g/L，VSS/SS 為 90% 以上，且具有高濃度的固體物（約 40 g/L）。本研究針對台北及高雄兩市的廚餘分別進行 7 次及 27 次的採樣與特性分析，並估算廚餘的電子分布。油脂及固態的蛋白質佔廚餘電子分布的最大宗，兩者的和超過總電子數的一半，而總碳水化合物所佔的比例則相對較小，北高兩市分別為 7.7% 及 19.3%。揮發酸的分布兩市相去不大，均約為 10% 左右，而揮發酸中，又以乳酸的濃度最高，高雄市廚餘乳酸的濃度可達 10 g/L。
　　本研究以一實驗室規模的 3 L 廚餘厭氧氫醱酵槽來進行其廚餘水解、酸化、產氫之程序研究，反應槽的形式定義為「間歇性進流完全攪拌反應槽」（Intermittent - Continuous Stirred Tank Reactor, I-CSTR）。廚餘基質在濃度及組成成分的變動造成其產氫速率上亦有明顯的起伏，在經過連續 300 天的長期操作，於第五試程發現有最高的平均體積產氫速率為 3.36±0.86 L H2/L-day，該試程亦操作在最高的體積荷負：100.5±24.8 g COD/L-day。最高的產氫 yield 及比產氫速率則皆發生在第四試程，分別為 96.4±37.9 mL H2/g VSSin 及 0.11±0.03 L H2/g VSS-day。
　　經過 277 天的廚餘氫醱酵微生物馴養，於第四試程將 I-CSTR 反應槽的出流混合液進行不同食微比的廚餘生化產氫潛能批分次試驗，由實驗的結果得知，在初始食微比為 S0/X0 = 9.5 g COD/g VSS 有最高的比產氫速率（specific hydrogen production rate）478 mL H2/g VSS-day，相較於反應槽啟動之際初始植種的污泥活性（150 mL H2/g VSS-day，初始食微比為 10.8 g COD/g VSS），有 3.19 倍的大幅增加。
　　以 I-CSTR 廚餘厭氧氫醱酵槽出流混合液進行 16S rRNA 基因選殖（clone library）及 DNA 定序（sequencing）實驗，在挑選的 154 個 clones 中發現 17 個 OTUs（operational taxonomic units）。62% 的菌比對最接近為 Butyrivibrio fibrisolvens（相似度為 92%），另有比對到兩株 Clostridium，分別為 Clostridium aminophilum（相似度為94%）及 Clostridum proteoclasticum（相似度為 91%），兩者均約佔 5%。在 I-CSTR 廚餘厭氧氫醱酵槽 12 次的尾端修飾限制片段長度多樣性（terminal restriction fragment length polymorphism, T-RFLP）圖譜分析中，發現反應槽內的微生物在不同操作條件下，其微生物菌相的組成有極大的變化。利用兩端引子修飾不同螢光染劑的改良式 T-RFLP，可應用於廚餘氫醱酵微生物探討並提高其對不同菌種的解析能力。

　　In Taiwan, almost one third of population contributed 1,500 tons per day of household kitchen waste that was reused till 2006. Kitchen waste in Taiwan was mainly reused for pig house feeding (75%) and composting (22%), but both of these two ways to treat the kitchen waste would cause some problems, such as infectious disease or secondary pollution. Anaerobic fermentation is one of the most environmentally friendly methods to treat the kitchen waste. In another hand, Taiwan was short of fossil fuel (coal, petroleum, natural gas etc.), and more than 98% of energy source was imported from other countries. Thus anaerobic fermentation might be the better method to treat the kitchen waste for both environment and energy consideration.
　　Kitchen waste was a kind of organic waste that contained with highly nutritive composition (total COD was about 82-107 g/L and VSS/SS was great than 90%) and with about 40 g/L high concentration of suspended solid. The electron distribution of kitchen waste was calculated by characteristic analysis for Taipei City and Kaohsiung city (n=7 and 27 respectively). Lipid and solid protein were the most dominant items of all which contained more than 50% of electron while total carbohydrate only about 7.7 to 19.8%. Electron distribution of VFAs (volatile fatty acids) was about 10% which mainly contributed by lactic acid.
　　A 3 L bench scale of bio-hydrogenation I-CSTR (Intermittent - Continuous Stirred Tank Reactor) was established to study kitchen waste treatment and bioenergy process with anaerobic fermentation. Large fluctuation of organic loading from kitchen waste attained to the high variation of biogas profile. Within 300 days of continuously long-term operation, the maximum average hydrogen production rate was observed in run 5 up to 3.36±0.86 L H2/L-day with the extremely high volumetric loading rate 100.5±24.8 g COD/L-day. The highest average hydrogen yield and specific hydrogen production rate was observed in run 4 with 96.4±37.9 mL H2/g VSSin and 0.11±0.03 L H2/g VSS-day, respectively.
　　After 277-days enrichment of microbial existed in I-CSTR, the bio-activity (specific hydrogen production rate) measured by batch test was increased 3.19 folds with 478 mL H2/g VSS-day where S0/X0 = 9.5 g COD/g VSS than previous study when inoculum (150 mL H2/g VSS-day, 10.8 g COD/g VSS).
　　As the result of clone library of 16S rRNA of the anaerobic microbes taken from I-CSRT bio-hydrogen reactor, there were 17 operational taxonomic units (OTUs) in 154 clones. 62% of clones could be identified as belonging to Butyrivibrio fibrisolvens (92% similarity) and both 5% of clones were Clostridium aminophilum (94% similarity) and Clostridum proteoclasticum (91% similarity). Significant population shift was observed through different runs by 12 T-RFLP analyses which total DNA extracted from mixed liquid of effluent in I-CSTR. Modified T-RFLP with both fluorescently labeled forward and reverse primers could improve the differential of bacteria existed in this reactor.

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