本論文利用假單胞菌(Pseudomonas putida)對混合抑制劑的瞬時耗氧速率來探討混合毒性之模式及聯合毒性；所選用之毒物為酚類以及苯胺類化合物皆為非競爭型抑制劑，任取兩個化合物混合且彼此間不發生反應。將實驗所得到的抑制速率數據代入動力學公式可以求得混合抑制劑之混合模式。並提出作圖法以及求值法表示聯合毒性，所得到之結果為：若在各種不同混合濃度抑制效果達50%時，A濃度增加的Ki,A之倍率與B濃度減少的Ki,B之倍率作圖成一直線，代表混合物為線性加成，且藉由作圖法之相關係數平方值即可判斷數據優劣，並可利用求值法確認所得到之結果。其中作圖法最為準確且容易判斷其結果。

Using PIOU ( Pseudomonas Initial Oxygen Uptake ) assay,we chose substituted phenols and substituted anilines as toxicants andinvestingatd the model of joint toxicity effect of compound mixture.. All of this toxicants are noncompetitive inhibitors and any two of them won’t react.
We proposed diagrammatic method and evaluated the Ki value to express joint toxicity. In this study, when the inhibited effect reaches 50% in different concentrations, the multiple of increasing Ki,A versus the multiple of decreasing Ki,B is linear and their joint toxicity is simple additive.
We can determine the value is good or not by R square value, and check the results with evaluating the Ki value.

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