微藻具有高二氧化碳吸收率、生長速率與光能利用率，因此普遍應用於生產生質能源。本論文建立以微藻油脂為原物料的生質柴油製程，以及用微藻中的碳水化合物進行發酵作用產生的生質酒精製程，生產大規模的生質燃料。本研究的第一部分是利用Aspen Plus軟體及靈敏度分析找出最適合的蒸餾塔操作條件。第二部分為製程的經濟分析，微藻培養經濟模式採用Benemann與Oswald(1996年)的方法，生質燃料製程部分採用J.M. Douglas(1988年)年出版的化工程序設計書目中的係數估計法，進行經濟可行性評估，計算製程投資報酬率，且分析設備成本、操作成本與營收變動所造成的影響，結果顯示製程投資報酬率(ROI)為43.5%、內部報酬率(IRR)為29.21%。第三部分為製程生命週期評估，探討製程中等量溫室氣體(eCO2)排放量，功能單位採用每單位能量生質燃料(MJ biofuel)，結果顯示，生質柴油溫室氣體排放(Greenhouse gas emission)為0.04717 kg eCO2/MJ、生質酒精為0.403kg eCO2/MJ，前者低於文獻中其他原物料溫室氣體排放值，顯示微藻作為生質燃料替代能源原物料具有很大的潛力。

Microalgae is recently a potential and desirable species for producing renewable fuel due to its high growth rate and carbon sequestration efficiency. In this research, Aspen Plus® software is used to simulate the biodiesel process with transesterification reaction and biodiesel process with fermentation reaction. The raw materials of the transesterification reaction and fermentation reaction are microalgae oil and carbohydrate, respectively. The optimized distillation column design is based on the sensitivity analyses to find the number of stages, feed stage, and reflux ratio. In the extractive distillation method using Ethylene glycol, glycerol acted as the entrainer can increase the concentration of azeotrope ethanol to 99.5 wt%. The best entrainer molar composition is found at 0.38 EG and 0.62 glycerol. Economic evaluation of the total system is then carried out by factor estimation which the relative error is less than 30 %. Return of investment (ROI) is 43.5 % and internal rate of return (IRR) is 29.21 % in this research, profitability from both of them are greater than other investment. Finally, life cycle assessment range from well to tank (WTT) is 0.04717 kg e-CO2/MJ in biodiesel and 0.403 kg e-CO2/MJ in bioethanol.

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