3-胺基甲基兒茶酚可由2,3-二羥基苯甲醛與無水的氨縮合成3-亞胺基甲基兒茶酚，再經氫化而製備。
經由競爭型態分析後發現，3-胺基甲基兒茶酚與偏鄰苯二酚雙加氧酶的受質兒茶酚競爭同一個活性部位；且經偏鄰苯二酚雙加氧酶催化反應後掃瞄其波長300nm至600nm範圍內的吸收，發現在波長395 nm處有強烈的吸收，故推測其為偏鄰苯二酚雙加氧酶的受質(KM = 21.42 mM)。
當3-胺基甲基兒茶酚與偏鄰苯二酚雙加氧酶共同馴養時，酵素活性隨時間衰退，且該抑制反應為假一級反應，因此推測3-胺基甲基兒茶酚為偏鄰苯二酚雙加氧酶的基理型抑制劑，其KI = 39.85 mM，kinact = 1.29 min-1。
3-胺基甲基兒茶酚抑制偏鄰苯二酚雙加氧酶的反應機構仍須更進一步的光譜證明來說明。

The target substrate 3-aminomethylcatechol was prepared from imine derivative, which was in turn synthesized from the condensation of 2,3-dihydroxybenzaldehyde with ammonia.
3-aminomethylcatechol was proved to be a competitive inhibitor of metapyrocatechase. The enzyme mediated oxidation product was showed an intense absorption at 395 nm with a KM of 21.42 mM.
While incubated the metapyrocatechase with 3-aminomethylcatechol, the result revealed a a pseudo first-order time-dependent loss of enzyme activity, this result indicated that 3-aminomethylcatechol was a mechanism-based inactivatorof metapyrocatechase (KI = 39.85 mM，kinact = 1.29 min-1). A plausible inactivation mechanism was proposed.

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