肌酸酐 (Creatinine) 是人體中十分重要的生理指標，其為體內肌酸的代謝最終產物。當血液流經腎臟時，腎絲球會過濾肌酸酐，並讓其隨尿液排出體內，因此藉由量測血液與尿液肌酸酐濃度可以反映人體腎臟功能的正常與否。本研究使用肌酸酐作為模版分子，3-aminopropyltriethoxysilane (APTES) 與 N-[3-(trimethoxysilyl)-propyl]aniline (TMOSPA) 作為功能性單體，tetraethyl orthosilicate (TEOS) 作為交聯劑，利用溶膠凝膠聚合法聚合形成高分子膜，之後以溶劑將肌酸酐洗脫，產生對肌酸酐具有特異性吸附效果之模版高分子 (molecularly imprinted polymer, MIP) 薄膜電極。從 scanning electron microscope (SEM) 可以鑑定出此高分子膜之存在，其厚度為9.35 μm。本研究分別以 Fourier transform infrared spectroscope (FT-IR) 與 electrochemical impedance spectroscope (EIS) 分析確認肌酸酐自 MIP 膜之脫附。由於肌酸酐無電化學活性，故後續以交流阻抗分析法感測不同濃度肌酸酐下之電阻抗訊號，製作肌酸酐檢量線，並與非模板高分子 (non-molecularly imprinted polymer, NIP) 薄膜電極比較，計算得其阻抗模印因子為 2.20，相位模印因子為40.0。另外此 MIP 電極對於肌酸酐共存物creatine 與相似物 N-hydroxysuccinimide 無明顯訊號變化，在此兩者干擾下，MIP 對肌酸酐仍保有感測效果，證明此 MIP 電極對肌酸酐之吸附對干擾物及共存物均具有選擇性。同時，此 MIP 電極可至少可重複使用 7 次。
本論文也接續先前實驗室研究成果，合成單用 TMOSPA 作為功能性單體之 s-MIP 薄膜電極，發現其可感測肌酸酐濃度，偵測範圍跨越尿液肌酸酐濃度 (30~270 mg/dL)。搭配商用網印電極進行 s-MIP 感測層之鋪覆，可製備出微小化肌酸酐感測器，僅使用少量的肌酸酐溶液 (1 μL) 即可偵測。從以上實驗結果可知 MIP 或s-MIP 電極對肌酸酐確實都具有特異性吸附，可有效感測肌酸酐，未來可整合微電子元件，製成穿戴式微小肌酸酐生醫感測晶片，具有協助臨床診斷腎臟疾病之潛力。

Creatinine, the waste of the creatine metabolism, is a very important physiological indicator. By measuring the serum as well as the urine creatinine concentration, we can judge the renal disease. This research take creatinine as template molecular, 3-aminopropyltriethoxy-silane (APTES) and N-[3-(trimethoxysilyl)-propyl]aniline (TMOSPA) as functional monomers, tetraethoxysilane (TEOS) as crosslinker, fabricating the polymer film via sol-gel process. After the extraction of creatinine by solvent, we can get the molecularly imprinted polymer (MIP) thin film electrode which have the specific up-taking effect toward creatinine. Owing to the lacking in electrochemical activity of creatinine, this research try to detect the creatinine concentration via AC impedance analysis, and get the calibration curve. Compared with the non-molecularly imprinted polymer (NIP) thin film electrode, the impedance imprinting factor is up to 2.20. For selectivity, the MIP electrode doesn’t show significant signal changing when detecting creatine and N-hydroxysuccinimide; moreover, when suffering from the interference of them, the MIP electrode still maintain the detecting effect, which imply that the MIP electrode can detect the creatinine specifically. This essay also only use the TMOSPA as functional monomer to fabricated s-MIP thin film electrode. We find that the s-MIP can detect the creatinine as well, and the detection range include the urine creatinine concentration range (30~270 mg/dl). By fabricating the s-MIP sensing layer onto the screen-printed electrode (SPE), we can get the microminiaturized creatinine sensor which can be used by just 1 μl creatinine solution.

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