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研究生： 李佳蓉
Li, Chia-Jung
論文名稱： 桌上型糞便潛血全自動定量免疫分析儀開發
Development of Desktop Automated Immunoanalyzer for Quantitative Fecal Occult Blood Screening Test
指導教授： 林裕城
Lin, Yu-Cheng
學位類別： 碩士
Master
系所名稱： 工學院 - 工程科學系
Department of Engineering Science
論文出版年： 2016
畢業學年度： 105
語文別： 中文
論文頁數： 116
中文關鍵詞： 自動化 、快速檢測 、可程式控制器PLC 、糞便潛血 、影像處理 、五滴孔卡匣
外文關鍵詞： rapid test system, QR code, fecal occult blood, image processing, desktop
相關次數： 點閱：100  下載：0 
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    • 本研究成功開發出具可大量分析、快速、高靈敏度及易操作特色之桌上型糞便潛血全自動定量免疫分析儀；在硬體方面利用滾珠螺桿、伺服馬達、高精準度移液系統、輸送帶以及廢棄盒設計出桌上型糞便潛血全自動定量免疫分析儀主體，並使用可程式控制器(Programmable Logic Controller, PLC)撰寫出檢測步驟程式；軟體部分為光學檢測模組並裝載Advanced RISC Machine (ARM)開發板、Universal Serial Bus (USB)攝影機、LED光源、LCD觸控式液晶顯示器、整合式電路板及光學暗房。在檢測程式方面使用Python整合模組Zbar、OpenCV、NumPy及Matplotlib撰寫出影像處理程式，並由觸控式螢幕顯示檢測資訊。最後使用ARM-¬_Raspberry pi (樹莓派)將硬體、軟體做整合，開發出桌上型糞便潛血全自動定量免疫分析儀。由研究結果可發現，開發之桌上型糞便潛血全自動定量免疫分析儀可精準達到完整檢測自動化之目的，並在滴入五滴孔卡匣上之糞便潛血檢測試劑下，能正確建立出線性度極佳之濃度與灰階值校正曲線，作為量化分析依據，該曲線決定係數可達0.99，在重複實驗下仍可保持良好的精準度。

    This thesis presents a highly sensitive and stable desktop automated immunoanalyzer system for fecal occult blood tests (FOB) . The inspection is based on immunochromatographic assay, which is widely applied to fecal occult blood detection. Combined with the immunochromatographic strip, the desktop automated immunoanalyzer system is able to detect and calculate the concentration of sample. The system, designed by SolidWorks, is composed of hardware and software. The analyzing programs include a main program, QR code, and image processing program compiled by Python.

    摘要 I Extended Abstract II 誌謝 V 縮寫表 VI 目錄 VIII 圖目錄 XIII 表目錄 XVIII 第一章 緒論 1 1-1 研究背景 1 1-2 大腸癌的檢測方式簡介 3 1-3 免疫分析法 11 1-3-1 免疫分析基本理論 12 1-3-2 抗原與抗體定義 12 1-3-3 抗原與抗體結合力 14 1-3-4 免疫分析檢測種類 15 1-3-5 免疫分析偵測方法 16 1-4 側流層析免疫反應試紙 19 1-5 免疫膠體金技術原理 22 1-6 檢測自動化簡介 25 1-7 影像判讀文獻回顧 27 1-7-1 影像判讀簡介 27 1-7-2 影像判讀之發展 29 1-8 研究動機與目的 33 1-9 章節架構 35 第二章 桌上型糞便潛血全自動定量免疫分析儀之設計與製作 37 2-1 桌上型糞便潛血全自動定量免疫分析儀原始程式 37 2-1-1 影像處理之流程 38 2-1-2 讀取C、T線之演算法 42 2-2 桌上型糞便潛血全自動定量免疫分析儀設計 44 2-2-1 桌上型糞便潛血全自動定量免疫分析儀系統流程 46 2-2-2 電路板設計 48 2-3 桌上型糞便潛血全自動定量免疫分析儀製作 49 2-3-1 整合式電路板 49 2-3-2 桌上型糞便潛血全自動定量免疫分析儀設備 49 2-4 程式開發環境建立 51 2-4-1 檢測路徑程式撰寫：PLC可程式控制器 51 2-4-2 開發板選用：ARM_Raspberry pi 52 2-4-3 作業系統選用：Raspbian 54 2-4-4 程式語言選用：Python 55 第三章 實驗與研究方法 57 3-1 實驗儀器 57 3-1-1 桌上型糞便潛血全自動定量免疫分析儀架構 57 3-1-2 XYZ軸控制系統 58 3-1-3 高精準度移液系統 58 3-1-4 五滴孔卡匣設計 60 3-1-5 檢體(Sample)放置盒 61 3-1-6 微量吸管(Tip)放置 62 3-1-7 黑箱設計 63 3-1-8 感光元件 64 3-1-9 LED光源 65 3-1-10 光感應器 66 3-2 實驗藥品與配製方法 68 3-3 設備及軟體測試 70 3-3-1桌上型糞便潛血全自動定量免疫分析儀介面整合測試 70 3-3-2 XYZ軸控制模組之距離測試 70 3-3-3 高精準度移液系統驗證測試 71 3-3-4 灰階線性度測試 74 3-3-4-1 灰階線性度測試 74 3-3-4-2 灰階靈敏度測試 75 3-4 桌上型糞便潛血全自動定量免疫分析儀整合測試 77 3-4-1 市售FOB試紙測試 77 第四章 結果與討論 78 4-1 檢測自動化之實現 78 4-1-1 桌上型糞便潛血全自動定量免疫分析儀介面整合 79 4-2 XYZ軸控制模組之距離測試結果 86 4-3 高精準度移液系統驗證測試結果 91 4-4 灰階線性度測試結果 92 4-4-1 灰階線性度測試結果 92 4-4-2 灰階靈敏度測試結果 99 4-5 桌上型糞便潛血全自動定量免疫分析儀測試結果 105 4-5-1 FOB試紙測試結果 105 4-5-2 導入擬合曲線之濃度測試結果 108 第五章 結論與建議 109 5-1 結論 109 5-2 建議 111 參考文獻 112

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